CRISPR-Cas9-mediated mutagenesis of the flowering repressor gene VcCENTRORADIALIS (VcCEN) induces early flowering in tetraploid highbush blueberry DOI Creative Commons
Masafumi Omori, Hisayo Yamane, Keishi Osakabe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 7, 2024

Abstract Flowering marks the vegetative-to-reproductive growth transition and is most important event in plant life cycle. Unlike annual plants, perennial fruit trees flower set fruits only after an extended juvenile phase (i.e., several years), which impediment to efficient breeding gene function analyses. In this study, we generated early flowering blueberry line via CRISPR-Cas9-mediated mutagenesis of VcCENTRORADIALIS (VcCEN). The expression VcCEN apical bud was negatively correlated with formation. Moreover, cultivar that flowers both autumn spring, VcCEN expression level lower decreased earlier than normal spring. data suggested functions as a repressor. CRISPR-Cas9 vector harboring gRNA targeting VcCENwas introduced into genome Agrobacterium-mediated transformation. Mutations (e.g., 1–10 bp indels) were detected stable transformants, all alleles tetraploid mutated some lines. Compared wild-type (WT), cen mutants exhibited repressed vegetative growth. Additionally, mutants, first occurred within 1 year Agrobacterium infection, approximately 1–2 years WT. single terminal without entering dormancy, whereas WT produced multiple axillary bloomed exposure chilling conditions then warm temperatures. This trait conducive functional analyses, especially crops long phase.

Язык: Английский

Enhancing the productivity and resilience of rice (Oryza sativa) under environmental stress conditions using clustered regularly interspaced short palindromic repeats (CRISPR) technology DOI
Aamir Riaz, Muhammad Uzair, Ali Raza

и другие.

Functional Plant Biology, Год журнала: 2025, Номер 52(1)

Опубликована: Янв. 2, 2025

Rice (Oryza sativa ) is a crucial staple crop worldwide, providing nutrition to more than half of the global population. Nonetheless, sustainability grain production increasingly jeopardized by both biotic and abiotic stressors exacerbated climate change, which increases crop's rvulnerability pests diseases. Genome-editing clustered regularly interspaced short palindromic repeats CRISPR-associated Protein 9 (CRISPR-Cas9) presents potential solution for enhancing rice productivity resilience under climatic stress. This technology can alter plant's genetic components without introduction foreign DNA or genes. It has become one most extensively used approaches discovering new gene functions creating novel varieties that exhibit higher tolerance stresses, herbicide resistance, improved yield production. study examines numerous CRISPR-Cas9-based genome-editing techniques knockout, knock-in, multiplexing simultaneous disruption multiple genes, base-editing, prime-editing. review elucidates application technologies enhance directly targeting yield-related genes indirectly modulating stress-responsive We highlight need integrate advancements with conventional advanced agricultural methods create are resilient thereby safeguarding food security promoting amid concerns.

Язык: Английский

Процитировано

1
Advancing vegetable genetics with gene editing: a pathway to food security and nutritional resilience in climate-shifted environments DOI
Rajib Roychowdhury,

Soumya Prakash Das,

Siddhartha Das

и другие.

Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Applications and Potential of Genomics and Genome Editing in Oil Crops Improvement DOI

Dhanya Mol T. Saraswathy,

M. M. Viji,

T. Dennis Thomas

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Molecular tools for genome editing in plants: a synthetic overview DOI Creative Commons
Nicolau Brito da Cunha, Michel Lopes Leite, Júlio Carlyle Macedo Rodrigues

и другие.

Vegetable Research, Год журнала: 2025, Номер 5(1), С. 0 - 0

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Development of an efficient and heritable virus-induced genome editing system in Solanum lycopersicum DOI Creative Commons

H.-J. Lee,

Jong Sam Baik,

Kyung-Nam Kim

и другие.

Horticulture Research, Год журнала: 2024, Номер unknown

Опубликована: Дек. 28, 2024

The CRISPR-Cas9 system can be used to introduce site-specific mutations into the genome of tomato (Solanum lycopersicum) plants. However, direct application this revolutionary technology desirable cultivars has been hindered by challenges generating transgenic To address issue, we developed an efficient and heritable editing using tobacco rattle virus (TRV) for elite cultivar (the paternal line Saladette). Notably, virus-induced (VIGE) enables rapid production various mutant seeds without need additional plant transformation tissue culture, once a Cas9-expressing is established. This VIGE consists plants that express Cas9 under control ubiquitin 10 (SlUbi10) gene promoter mobile guide RNA scaffold (gRNA:SlmFT) generated sequence Flowering Locus T (SlFT) gene. We determined its efficiency targeting phytoene desaturase (SlPDS) gene, which causes photobleaching symptoms when disrupted. Most seedlings infected with TRV vectors carrying SlPDS-targeting sgRNA chimeric albino leaves associated high frequency indel in SlPDS Remarkably, fruits from these yielded homozygous knockout at rates ranging 15% 100%. These results demonstrate exceptional effectiveness our rapidly edits tomato.

Язык: Английский

Процитировано

2
Update on functional analysis of long non-coding RNAs in common crops DOI Creative Commons
Aijing Zhang,

Wenxuan Pi,

Yashuo Wang

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Май 30, 2024

With the rapid advances in next-generation sequencing technology, numerous non-protein-coding transcripts have been identified, including long noncoding RNAs (lncRNAs), which are functional comprising more than 200 nucleotides. Although lncRNA-mediated regulatory processes extensively investigated animals, there has considerably less research on plant lncRNAs. Nevertheless, multiple studies major crops showed lncRNAs involved crucial processes, growth and development, reproduction, stress responses. This review summarizes progress lncRNA roles several crops, presents key strategies for exploring discusses current challenges future prospects. The insights provided this will enhance our comprehension of functions with potential implications improving crop genetics breeding.

Язык: Английский

Процитировано

1
Cutting-Edge Genome Editing in Sugarcane and Sugar Crops: A Comprehensive Overview DOI
Mayla Daiane Corrêa Molinari, Renata Fuganti‐Pagliarini, Natália Chagas Freitas

и другие.

Sugar Tech, Год журнала: 2024, Номер unknown

Опубликована: Авг. 9, 2024

Язык: Английский

Процитировано

1
Targeted mutation of BnaMS1/BnaMS2 combined with the RUBY reporter enables an efficient two-line system for hybrid seed production in Brassica napus DOI Creative Commons

Xiaoxiao Shen,

Qing Dong, Xiang Zhao

и другие.

Horticulture Research, Год журнала: 2024, Номер 12(1)

Опубликована: Сен. 25, 2024

Abstract The recessive genic male sterility (RGMS) method has several benefits in hybrid seed production; however, it is seldom employed industrial production owing to the difficulty of producing an ample number pure male-sterile seeds. In this study, we present efficient methodology for developing a two-line strategy produce through targeted mutation BnaMS1 and BnaMS2 conjunction with RUBY reporter Brassica napus. method, lines were successfully created directly from different elite rapeseed breeding CRISPR/Cas9-mediated mutagenesis enhanced Agrobacterium-mediated transformation. To establish transgenic maintainer, three technology (SPT) cassettes carrying functional gene linked reporters (DsRed, BnaA07.PAP2, RUBY) tested compared rapeseed. results indicated that PMR-based possesses advantages such as phenotypic stability ease identification at early stages, making ideal tool rapid screening. Subsequently, maintainer single hemizygous copy SPT cassette developed context Bnams1Bnams2 double mutants. progeny crossing line its counterpart exhibited 1:1 segregation pattern nontransgenic male-fertile plants, distinguishable by seedling color. This biotechnological approach offers promising prospects improving propagation plants development seeds Furthermore, simple adapt technique more crops.

Язык: Английский

Процитировано

1
Context effects on repair of 5′‐overhang DNA double‐strand breaks induced by Cas12a in Arabidopsis DOI Creative Commons

Sébastien Lageix,

Miguel Hernández Sánchez-Rebato, Maria E. Gallego

и другие.

Plant Direct, Год журнала: 2024, Номер 8(10)

Опубликована: Окт. 1, 2024

Sequence-specific endonucleases have been key to the study of mechanisms and control DNA double-strand break (DSB) repair recombination, availability CRISPR-Cas nucleases over last decade has driven rapid progress in understanding application targeted recombination many organisms, including plants. We present here an analysis at chromosomal 5' overhang DSB generated by FnCas12a endonuclease plant,

Язык: Английский

Процитировано

1
Context effects on repair of 5’-overhang DSB induced by Cas12a in Arabidopsis DOI Creative Commons

Sébastien Lageix,

Miguel Hernández Sánchez-Rebato, Maria E. Gallego

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 5, 2024

Abstract Sequence-specific endonucleases have been key to the study of mechanisms and control DNA double-strand break (DSB) repair recombination availability CRISPR-Cas nucleases over last decade has driven rapid progress in understanding application targeted many organisms, including plants. We present here an analysis at chromosomal 5’overhang DSB generated by FnCas12a endonuclease plant, Arabidopsis thaliana . The much-studied Cas9 nuclease cleaves generate blunt-ended, breaks (DSB), but relatively less is known about other types breaks, such as those with 5’-overhanging ends. Sequencing repaired clearly shows that majority carry small deletions are thus locally End-Joining recombination, confirmed Nanopore sequencing larger amplicons. Paired one or both cut-sites, well reinsertions deleted segment between two cuts, visible inversions. While differences seen details, overall deletion patterns similar single-cut double-cut events, notwithstanding fact only former involve cohesive overhangs. A strikingly different pattern however observed flanked direct repeats. This change sequence context results presence alternative class corresponding highly efficient Single-strand Annealing recombination.

Язык: Английский

Процитировано

0