CRISPR-Cas9-mediated mutagenesis of the flowering repressor gene VcCENTRORADIALIS (VcCEN) induces early flowering in tetraploid highbush blueberry DOI Creative Commons
Masafumi Omori, Hisayo Yamane, Keishi Osakabe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 7, 2024

Abstract Flowering marks the vegetative-to-reproductive growth transition and is most important event in plant life cycle. Unlike annual plants, perennial fruit trees flower set fruits only after an extended juvenile phase (i.e., several years), which impediment to efficient breeding gene function analyses. In this study, we generated early flowering blueberry line via CRISPR-Cas9-mediated mutagenesis of VcCENTRORADIALIS (VcCEN). The expression VcCEN apical bud was negatively correlated with formation. Moreover, cultivar that flowers both autumn spring, VcCEN expression level lower decreased earlier than normal spring. data suggested functions as a repressor. CRISPR-Cas9 vector harboring gRNA targeting VcCENwas introduced into genome Agrobacterium-mediated transformation. Mutations (e.g., 1–10 bp indels) were detected stable transformants, all alleles tetraploid mutated some lines. Compared wild-type (WT), cen mutants exhibited repressed vegetative growth. Additionally, mutants, first occurred within 1 year Agrobacterium infection, approximately 1–2 years WT. single terminal without entering dormancy, whereas WT produced multiple axillary bloomed exposure chilling conditions then warm temperatures. This trait conducive functional analyses, especially crops long phase.

Язык: Английский

CRISPR-Cas9-mediated mutagenesis of the flowering repressor gene VcCENTRORADIALIS (VcCEN) induces early flowering in tetraploid highbush blueberry DOI Creative Commons
Masafumi Omori, Hisayo Yamane, Keishi Osakabe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 7, 2024

Abstract Flowering marks the vegetative-to-reproductive growth transition and is most important event in plant life cycle. Unlike annual plants, perennial fruit trees flower set fruits only after an extended juvenile phase (i.e., several years), which impediment to efficient breeding gene function analyses. In this study, we generated early flowering blueberry line via CRISPR-Cas9-mediated mutagenesis of VcCENTRORADIALIS (VcCEN). The expression VcCEN apical bud was negatively correlated with formation. Moreover, cultivar that flowers both autumn spring, VcCEN expression level lower decreased earlier than normal spring. data suggested functions as a repressor. CRISPR-Cas9 vector harboring gRNA targeting VcCENwas introduced into genome Agrobacterium-mediated transformation. Mutations (e.g., 1–10 bp indels) were detected stable transformants, all alleles tetraploid mutated some lines. Compared wild-type (WT), cen mutants exhibited repressed vegetative growth. Additionally, mutants, first occurred within 1 year Agrobacterium infection, approximately 1–2 years WT. single terminal without entering dormancy, whereas WT produced multiple axillary bloomed exposure chilling conditions then warm temperatures. This trait conducive functional analyses, especially crops long phase.

Язык: Английский

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