The
increasing
rate
of
cardiovascular
disease
(CVD)
worldwide
contributes
to
a
worsening
quality
life
in
surviving
patients
and
socioeconomic
burden
on
the
health
care
system.
There
is
currently
no
cure
for
CVD.
innovation
novel
therapies
has
traditionally
been
significantly
stalled
due
several
obstacles,
such
as
complications
resulting
from
scar
tissue
formation
need
representative
patient-specific
cardiac
model.
However,
last
two
decades
have
seen
emergence
induced
pluripotent
stem
cells
(iPSCs),
which
risen
at
forefront
regenerative
medicine
research
cell
source.
In
this
chapter,
we
will
cover
basics
reprogramming,
iPSC
generation,
assessment
before
moving
versatile
array
roles
that
iPSCs
hold.
We
outline
three
potential
uses
heart
regeneration
research:
precursor
an
vitro
model,
vivo
implantation
purposes,
source
secretome.
Finally,
aim
highlight
importance
incorporating
biological
characteristics
within
large-scale
identify
gaps
applications
medicine.
Antioxidants and Redox Signaling,
Год журнала:
2024,
Номер
40(7-9), С. 369 - 432
Опубликована: Фев. 1, 2024
Physiological
levels
of
reactive
oxygen
and
nitrogen
species
(ROS/RNS)
function
as
fundamental
messengers
for
many
cellular
developmental
processes
in
the
cardiovascular
system.
ROS/RNS
involved
cardiac
redox-signaling
originate
from
diverse
sources,
their
are
tightly
controlled
by
key
endogenous
antioxidant
systems
that
counteract
accumulation.
However,
dysregulated
redox-stress
resulting
inefficient
removal
leads
to
inflammation,
mitochondrial
dysfunction,
cell
death,
contributing
development
progression
disease
(CVD).
Basic Research in Cardiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Март 23, 2024
Abstract
Immune
checkpoint
inhibitors
(ICIs)
exhibit
remarkable
antitumor
activity
and
immune-related
cardiotoxicity
of
unknown
pathomechanism.
The
aim
the
study
was
to
investigate
ICI
class-dependent
in
vitro
pembrolizumab’s
(Pem’s)
vivo,
seeking
for
translational
prevention
means.
Cytotoxicity
investigated
primary
cardiomyocytes
splenocytes,
incubated
with
ipilimumab,
Pem
avelumab.
Pem’s
cross-reactivity
assessed
by
circular
dichroism
(CD)
on
biotechnologically
produced
human
murine
PD-1
silico.
C57BL6/J
male
mice
received
IgG4
or
2
5
weeks.
Echocardiography,
histology,
molecular
analyses
were
performed.
Coronary
blood
flow
velocity
mapping
cardiac
magnetic
resonance
imaging
conducted
at
Human
EA.hy926
endothelial
cells
Pem-conditioned
media
from
mononuclear
cells,
presence
absence
statins
viability
signaling
assessed.
Atorvastatin
(20
mg/kg,
daily)
administered
vivo
,
as
prophylaxis.
Only
exerted
cytotoxicity
vitro.
confirmed
CD
docking.
In
initiated
coronary
diastolic
dysfunction
weeks
systolic
At
weeks,
induced
ICAM-1
iNOS
expression
intracardiac
leukocyte
infiltration.
exacerbated
activation
triggered
inflammation.
led
which
prevented
atorvastatin.
mitigated
functional
deficits,
inhibiting
vivo.
We
established
first
time
an
model
Pem-induced
cardiotoxicity.
precedes
cardiotoxicity,
whereas
atorvastatin
emerges
a
novel
prophylactic
therapy.
Infection,
Год журнала:
2024,
Номер
52(4), С. 1269 - 1285
Опубликована: Фев. 7, 2024
Abstract
Introduction
SARS-CoV-2
infection
causes
severe
endothelial
damage,
an
essential
step
for
cardiovascular
complications.
Endothelial-colony
forming
cells
(ECFCs)
act
as
a
biomarker
of
vascular
damage
but
their
role
in
remain
unclear.
The
aim
this
study
was
to
assess
whether
the
number
ECFCs
and
angiogenic
biomarkers
remained
altered
after
6
12-months
post-infection
imbalance
correlated
with
presence
long-COVID
syndrome
other
biological
parameters
measured.
Methods
Seventy-two
patients
were
recruited
at
different
time-points
overcoming
COVID-19
thirty-one
healthy
controls.
All
subjects
matched
age,
gender,
BMI,
comorbidities.
obtained
from
peripheral
blood
cultured
specific
conditions.
Results
results
confirm
long-term
sequela
post-COVID-19
patients,
abnormal
increase
ECFC
production
compared
controls
(82.8%
vs
.
48.4%,
P
<
0.01)
that
is
maintained
up
6-months
(87.0%
(85.0%
vs.
0.01).
Interestingly,
showed
significant
downregulation
angiogenesis-related
proteins
indicating
clear
injury.
Troponin,
NT-proBNP
ferritin
levels,
markers
risk
inflammation,
elevated
post-infection.
Patients
lower
numbers
exhibited
higher
levels
inflammatory
markers,
such
ferritin,
suggesting
may
play
protective
role.
Additionally,
associated
female
gender.
Conclusions
These
findings
highlight
last
6-
point
out
need
preventive
measures
patient
follow-up.
Complex Issues of Cardiovascular Diseases,
Год журнала:
2025,
Номер
13(4), С. 191 - 203
Опубликована: Янв. 11, 2025
Highlights
A
fundamental
distinguishing
feature
of
normal
endothelial
cell
morphotypes
from
pathological
ones
is
their
preserved
orientation
along
the
direction
blood
flow
in
absence
cytoplasmic
or
membrane
defects.
The
main
characteristics
dysfunctional
cells
include
a
spherical
shape
(indicating
loss
cellular
flow),
presence
large
vacuoles
within
cell,
vacuolization,
impaired
plasma
integrity,
reduced
contrast
between
nucleus
and
cytoplasm,
partial
detachment
basement
membrane.
condition
organelles
(mitochondria,
Golgi
complex,
endoplasmic
reticulum)
integrity
are
not
sensitive
specific
markers
endothelium
compared
to
aforementioned
features
Aim.
To
analyze
electron
microscopic
using
descending
aorta
rats
(characterized
by
laminar
flow).
Methods.
study
was
conducted
on
5
male
Wistar
(age
≈
6
months,
body
weight
500
g).
extracted
aortas
were
chemically
fixed
2.5%
glutaraldehyde,
post-fixed
1%
osmium
tetroxide
solution
with
1.5%
potassium
ferrocyanide,
incubated
thiocarbohydrazide,
stained
2%
aqueous
solution,
contrasted
phosphotungstic
acid,
gadolinium
triacetate,
dehydrated
ascending
concentrations
ethanol,
isopropanol,
acetone,
embedded
mixture
acetone
epoxy
resin,
then
pure
Araldite
502
followed
its
polymerization.
After
grinding
polishing,
samples
lead
citrate,
coated
carbon,
visualized
backscattered
scanning
microscopy.
Results.
Electron
analysis
identified
three
cells:
1)
elongated
an
nucleus;
semicircular
oval,
round,
kidney-shaped,
polymorphic
less
pronounced
but
clearly
visible
flow;
3)
even
flow.
often
contained
various
Dysfunctional
also
exhibited
several
morphotypes,
characterized
different
combinations
following
features:
flow,
disruption
However,
changes
structure
adjacent
observed
cells.
Conclusion.
preservation
indicates
phenotype
other
signs
(vacuoles
membrane).
Abstract
Acute
myocardial
infarction
(AMI)
is
a
leading
cause
of
mortality
worldwide.
MicroRNAs
(miRNAs),
among
other
small
non-coding
RNAs,
shape
the
transcriptome
and
control
cellular
functions.
Although
single-cell
technologies
are
now
available
to
study
ischemia
response,
RNA
regulation
limited
by
depth
expression,
capture
efficiency
lack
full
coverage
transcripts.
In
addition,
kinetic
expression
miRNAs
unknown.
Using
paired
total
sequencing,
we
built
an
atlas
temporal
dynamics
genes
in
four
major
heart
cell
types
after
AMI.
Expression
reveal
enriched
functions
highlighting
type-specific
AMI
stress
responses.
Many
deregulated
mouse
overlap
with
known
human
cardiovascular
disease
genes.
The
dataset
highly
valuable
for
additional
research
on
long
such
as
variants
splicing
or
alternative
ORFs.
All
all,
provides
useful
resource
different
roles
RNAs
Basic Research in Cardiology,
Год журнала:
2023,
Номер
118(1)
Опубликована: Янв. 26, 2023
Long
non-coding
RNAs
(lncRNAs)
can
act
as
regulatory
which,
by
altering
the
expression
of
target
genes,
impact
on
cellular
phenotype
and
cardiovascular
disease
development.
Endothelial
lncRNAs
their
vascular
functions
are
largely
undefined.
Deep
RNA-Seq
FANTOM5
CAGE
analysis
revealed
lncRNA
LINC00607
to
be
highly
enriched
in
human
endothelial
cells.
was
induced
response
hypoxia,
arteriosclerosis
regression
non-human
primates,
post-atherosclerotic
cultured
cells
from
patients
also
propranolol
used
induce
arteriovenous
malformations.
siRNA
knockdown
or
CRISPR/Cas9
knockout
attenuated
VEGF-A-induced
angiogenic
sprouting.
integrated
less
into
newly
formed
networks
an
vivo
assay
SCID
mice.
Overexpression
CRISPR
restored
normal
function.
RNA-
ATAC-Seq
after
changes
transcription
gene
sets
linked
chromatin
accessibility
around
ERG-binding
sites.
Mechanistically,
interacted
with
SWI/SNF
remodeling
protein
BRG1.
CRISPR/Cas9-mediated
BRG1
HUVEC
followed
CUT&RUN
that
is
required
secure
a
stable
state,
mainly
In
conclusion,
endothelial-enriched
maintains
ERG
interacting
remodeler
ultimately
mediate
angiogenesis.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(17), С. 13268 - 13268
Опубликована: Авг. 26, 2023
Myocardial
infarction
(MI)
is
one
of
the
leading
causes
death
in
Western
countries.
An
early
diagnosis
decreases
subsequent
severe
complications
such
as
wall
remodeling
or
heart
failure
and
improves
treatments
interventions.
Novel
therapeutic
targets
have
been
recognized
and,
together
with
development
direct
indirect
epidrugs,
role
non-coding
RNAs
(ncRNAs)
yields
great
expectancy.
ncRNAs
are
a
group
not
translated
into
product
among
them,
microRNAs
(miRNAs)
most
investigated
subgroup
since
they
involved
several
pathological
processes
related
to
MI
post-MI
phases
inflammation,
apoptosis,
angiogenesis,
fibrosis.
These
pathways
finely
tuned
by
miRNAs
via
complex
mechanisms.
We
at
beginning
investigation
main
paths
still
underexplored.
In
this
review,
we
provide
comprehensive
discussion
recent
findings
on
epigenetic
changes
first
after
well
miRNAs.
focused
function
their
relationship
key
molecules
cells
healing
an
ischemic
accident,
while
also
giving
insight
discrepancy
between
males
females
prognosis
cardiovascular
diseases.
