Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease DOI Open Access
Elissavet Chartampila, Karim S. Elayouby, Paige Leary

и другие.

Опубликована: Май 15, 2024

Maternal choline supplementation (MCS) improves cognition in Alzheimer’s disease (AD) models. However, effects of MCS on neuronal hyperexcitability AD are unknown. We investigated a well-established mouse model with hyperexcitability, the Tg2576 mouse. The most common type mice, and many other models patients, generalized EEG spikes (interictal spikes; IIS). Hyperexcitability is also reflected by elevated expression transcription factor ΔFosB granule cells (GCs) dentate gyrus (DG), which principal cell type. studied hilus DG because hilar neurons regulate GC excitability. found reduced marker NeuN within studies have shown sign oxidative stress or pathology.Tg2576 breeding pairs received diet relatively low, intermediate high concentration choline. After weaning, all mice diet. In offspring fed diet, IIS frequency declined, was reduced, restored. Spatial memory improved using novel object location task. contrast, exposed to low had several adverse effects, such as increased mortality. They weakest immunoreactivity greatest ΔFosB. their surprising. results provide new evidence that early life can improve outcomes AD, mixed effects. This first study showing dietary neurons, spatial an animal AD.

Язык: Английский

Single cell transcriptome analysis identified a unique neutrophil type associated with Alzheimer’s disease DOI Creative Commons
Xiaolin Zhang,

Guiqin He,

Yixuan Hu

и другие.

Immunity & Ageing, Год журнала: 2024, Номер 21(1)

Опубликована: Июнь 25, 2024

Abstract Background Neutrophils play an essential role in Alzheimer’s disease (AD) pathology. However, the extent of their heterogeneity remains poorly explored, particularly context developing novel therapies targeting these cells. Results We investigate population structure neutrophils purified from peripheral blood samples AD mice. Utilizing single cell RNA sequencing, we comprehensively map neutrophil populations into six distinct clusters and find that Neu-5 subset is specially enriched This exhibits fewer specific granules a lower mature score. Gene ontology (GO) analysis reveals genes involved cytokine-mediated signaling are downregulated cluster. Furthermore, identify Ccrl2 gene specifically upregulated this subgroup, which confirmed by flow cytometry Finally, immunohistochemical staining indicates CCRL2 protein increased brains Conclusions unique positive cluster,

Язык: Английский

Процитировано

2
Daily Vinegar Ingestion Improves Depression and Enhances Niacin Metabolism in Overweight Adults: A Randomized Controlled Trial DOI Open Access

Haley Barrong,

H. Justin Coven,

Alexandra Lish

и другие.

Nutrients, Год журнала: 2024, Номер 16(14), С. 2305 - 2305

Опубликована: Июль 18, 2024

Depressive disorders are the most prevalent mental health conditions in world. The commonly prescribed antidepressant medications can have serious side effects, and their efficacy varies widely. Thus, simple, effective adjunct therapies needed. Vinegar, a fermented acetic acid solution, is emerging as healthful dietary supplement linked to favorable outcomes for blood glucose management, heart disease risk, adiposity reduction, recent report suggests vinegar may improve symptoms of depression. This randomized controlled study examined 4-week change scores Center Epidemiological Studies Depression (CES-D) questionnaire Patient Health Questionnaire (PHQ-9) healthy overweight adults ingesting 2.95 g (4 tablespoons vinegar) vs. 0.025 (one pill) daily. A secondary objective explored possible underlying mechanisms using metabolomics analyses. At week 4, mean CES-D fell 26% 5% VIN CON participants respectively, non-significant difference between groups, PHQ-9 42% 18% (p = 0.036). Metabolomics analyses revealed increased nicotinamide concentrations upregulation NAD+ salvage pathway compared controls, metabolic alterations previously improved mood. daily ingestion over four weeks self-reported depression symptomology adults, enhancements niacin metabolism factor into this improvement.

Язык: Английский

Процитировано

2
Association between choline supplementation and Alzheimer’s disease risk: a systematic review protocol DOI Creative Commons
Sixtus Aguree, Maryam Zolnoori, Thea Atwood

и другие.

Frontiers in Aging Neuroscience, Год журнала: 2023, Номер 15

Опубликована: Авг. 28, 2023

Background and aims There is growing evidence suggesting choline intake might have beneficial effects on cognitive function in the elderly. However, some studies report no relationship between or improvement Alzheimer’s disease patients. This protocol for a systematic review of that to assess comparative clinical effectiveness supplementation risk. Methods analysis literature search will be performed PubMed, MEDLINE, EMBASE, CINAHL, Scopus, Cochrane, Web Science electronic databases from inception until October 2023. We follow Preferred Reporting Items Systematic Reviews Meta-Analyses (PRISMA) guidelines. Studies included if they compared two different time points biomarkers measures men women (65+) with Disease. The risk bias assessed within Covidence data-management software. Results summarize trial quasi-experimental adults aged 65+. results all eligible presented tables, text, figures. A descriptive synthesis present characteristics (e.g., age, sex participants, type, length intervention comparator, outcome measures), critical appraisal results, descriptions main findings. Discussion existing association Choline AD make recommendations appropriate. this considered respect whether there enough benefit merit more definitive randomized controlled trial. disseminated through peer-reviewed journals population. Conclusion outlines methodology AD. resulting form an evidence-based foundation advance nutrition care individuals poor function. registration http://www.crd.york.ac.uk/PROSPERO , identifier CRD42023395004.

Язык: Английский

Процитировано

4
Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease DOI Creative Commons
Elissavet Chartampila, Karim S. Elayouby, Paige Leary

и другие.

eLife, Год журнала: 2024, Номер 12

Опубликована: Май 2, 2024

Maternal choline supplementation (MCS) improves cognition in Alzheimer’s disease (AD) models. However, the effects of MCS on neuronal hyperexcitability AD are unknown. We investigated a well-established mouse model with hyperexcitability, Tg2576 mouse. The most common type mice generalized EEG spikes (interictal [IIS]). IIS also other models and occur patients. In models, is reflected by elevated expression transcription factor ∆FosB granule cells (GCs) dentate gyrus (DG), which principal cell type. Therefore, we studied ΔFosB GCs. marker NeuN within hilar neurons DG because reduced protein sign oxidative stress or pathology. This potentially important regulate GC excitability. breeding pairs received diet relatively low, intermediate, high concentration choline. After weaning, all intermediate diet. offspring fed diet, frequency declined, was reduced, restored. Using novel object location task, spatial memory improved. contrast, exposed to low had several adverse effects, such as increased mortality. They weakest immunoreactivity greatest expression. their surprising. results provide new evidence that early life can improve outcomes AD, have mixed effects. first study showing dietary neurons, ΔFosB, an animal AD.

Язык: Английский

Процитировано

1
Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s disease DOI Open Access
Elissavet Chartampila, Karim S. Elayouby, Paige Leary

и другие.

Опубликована: Май 15, 2024

Maternal choline supplementation (MCS) improves cognition in Alzheimer’s disease (AD) models. However, effects of MCS on neuronal hyperexcitability AD are unknown. We investigated a well-established mouse model with hyperexcitability, the Tg2576 mouse. The most common type mice, and many other models patients, generalized EEG spikes (interictal spikes; IIS). Hyperexcitability is also reflected by elevated expression transcription factor ΔFosB granule cells (GCs) dentate gyrus (DG), which principal cell type. studied hilus DG because hilar neurons regulate GC excitability. found reduced marker NeuN within studies have shown sign oxidative stress or pathology.Tg2576 breeding pairs received diet relatively low, intermediate high concentration choline. After weaning, all mice diet. In offspring fed diet, IIS frequency declined, was reduced, restored. Spatial memory improved using novel object location task. contrast, exposed to low had several adverse effects, such as increased mortality. They weakest immunoreactivity greatest ΔFosB. their surprising. results provide new evidence that early life can improve outcomes AD, mixed effects. This first study showing dietary neurons, spatial an animal AD.

Язык: Английский

Процитировано

1