Replication-dependent histone labeling dissects the physical properties of euchromatin/heterochromatin in living human cells

Science Advances, Год журнала: 2025, Номер 11(13)

Опубликована: Март 28, 2025

A string of nucleosomes, where genomic DNA is wrapped around histones, organized in the cell as chromatin, ranging from euchromatin to heterochromatin, with distinct genome functions. Understanding physical differences between and heterochromatin crucial, yet specific labeling methods living cells remain limited. Here, we have developed replication-dependent histone (Repli-Histo) mark nucleosomes based on replication timing. Using this approach, investigated local nucleosome motion four known chromatin classes, human mouse cells. The more euchromatic (earlier-replicated) heterochromatic (later-replicated) regions exhibit greater lesser motions, respectively. Notably, profile each class persists throughout interphase. Genome essentially replicated although timing perturbed. Our findings, combined computational modeling, suggest that earlier-replicated accessibility, can be a major determinant genome-wide

Язык: Английский

---

The shifting paradigm of chromatin structure: from the 30-nm chromatin fiber to liquid-like organization

Proceedings of the Japan Academy Series B, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The organization and dynamics of chromatin are critical for genome functions such as transcription DNA replication/repair. Historically, was assumed to fold into the 30-nm fiber progressively arrange larger helical structures, described in textbook model. However, over past 15 years, extensive evidence including our studies has dramatically transformed view from a static, regular structure one that is more variable dynamic. In higher eukaryotic cells, forms condensed yet liquid-like domains, which appear be basic unit structure, replacing fiber. These domains maintain proper accessibility, ensuring regulation reaction processes. During mitosis, these assemble form gel-like mitotic chromosomes, further constrained by condensins other factors. Based on available evidence, I discuss physical properties live emphasizing its viscoelastic nature-balancing local fluidity with global stability support functions.

Язык: Английский

---

Euchromatin and heterochromatin: implications for DNA accessibility and transcription

Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169270 - 169270

Опубликована: Июнь 1, 2025

Язык: Английский

---

Seeing genomes

Histochemistry and Cell Biology, Год журнала: 2024, Номер 162(1-2), С. 1 - 2

Опубликована: Июнь 8, 2024

---