Photoreceptor calyceal processes accompany the developing outer segment, adopting a stable length despite a dynamic core

Journal of Cell Science, Год журнала: 2024, Номер 137(7)

Опубликована: Март 13, 2024

ABSTRACT Vertebrate photoreceptors detect light through a large cilium-based outer segment, which is filled with photopigment-laden membranous discs. Surrounding the base of segment are microvilli-like calyceal processes (CPs). Although CP disruption has been associated altered morphology and photoreceptor degeneration, role CPs remains elusive. Here, we used zebrafish as model to characterize CPs. We quantified parameters report strong disparity in coverage between subtypes. length stable across dark conditions, yet heat-shock inducible expression tagged actin revealed rapid turnover core. Detailed imaging embryonic retina uncovered substantial remodeling developing apical surface, including transition from dynamic tangential vertically oriented immediately prior formation. Remarkably, also found direct connection extensions Müller glia retinal pigment epithelium, arranged bundles around ultraviolet sensitive cones. In summary, our data structure, development surrounding environment microvilli retina.

Язык: Английский

---

Partitioning of gene expression among zebrafish photoreceptor subtypes

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Авг. 30, 2021

Abstract Vertebrate photoreceptors are categorized into two broad classes, rods and cones, responsible for dim- bright-light vision, respectively. While many molecular features that distinguish cones known, gene expression differences among cone subtypes remain poorly understood. Teleost fishes renowned the diversity of their photoreceptor systems. Here, we used single-cell RNA-seq to profile adult in zebrafish, a teleost. We found addition four canonical zebrafish types, there exist subpopulations green red (previously shown be located ventral retina) express red-shifted opsin paralogs ( opn1mw4 or opn1lw1 ) as well unique combination phototransduction genes. Furthermore, paralogous genes is partitioned subtypes, analogous partitioning between seen across vertebrates. The cone-gene pairs arose via teleost-specific whole-genome duplication later clade-specific duplications. also discovered distinct transcriptional regulators, including factors not previously implicated development differentiation. Overall, our work suggests action differentially expressed regulators enables diversification teleosts.

Язык: Английский

---

Modeling sacsin depletion in Danio Rerio offers new insight on retinal defects in ARSACS

Neurobiology of Disease, Год журнала: 2025, Номер unknown, С. 106793 - 106793

Опубликована: Янв. 1, 2025

Biallelic mutations in the SACS gene, encoding sacsin, cause early-onset autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS), a neurodegenerative disease also characterized by unique and poorly understood retinal abnormalities. While two murine models replicate phenotypic neuronal features observed patients, no phenotype has been described so far. In zebrafish knock-out strain that faithfully mirrors main aspects ARSACS, we impaired visual function due to photoreceptor degeneration, likely caused cell cycle defects progenitor cells. RNA-seq analysis embryos revealed dysfunction proteins related fat-soluble vitamins (e.g., TTPA, RDH5, VKORC) suggested key role neuroinflammation driving defects. Our findings indicate studying pathology ARSACS could be crucial for understanding impact sacsin depletion may offer insights into halting progression.

Язык: Английский

---

Liquid crystal monomers disrupt photoreceptor patterning of zebrafish larvae via thyroid hormone signaling

Environment International, Год журнала: 2024, Номер 188, С. 108747 - 108747

Опубликована: Май 13, 2024

Liquid crystal monomers (LCMs) are the raw material for liquid displays, and their use is steadily increasing in electronic products. Recently, LCMs have been reported to be novel endocrine disrupting chemicals, however, mechanisms underlying potential thyroid hormone disruption visual toxicity not well understood. In this study, six widely used fluorinated (FLCMs) were selected determine putative FLCM-induced zebrafish systems. Exposure FLCMs caused damage retinal structures reduced cell density of ganglion layer, inner nuclear photoreceptor layer approximately 12.6-46.1%. also disrupted levels perturbed hypothalamic-pituitary-thyroid axis by affecting key enzymes protein larvae. A hormone-dependent GH3 viability assay supported hypothesis that act as chemicals. It was determined containing aliphatic ring may a higher T3 antagonism compared without an ring. Molecular docking silico suggested affect biological functions thyroxine binding globulin, membrane receptor integrin, beta. Lastly, motor response red- green-light significantly inhibited following exposure FLCMs. Taken together, we demonstrate can disruptors induce dysfunction via several molecular mechanisms.

Язык: Английский

---

Bio-inspired tunable optics and photonics: bridging the gap between nature and technology

International Journal of Optomechatronics, Год журнала: 2024, Номер 18(1)

Опубликована: Апрель 14, 2024

In the realm of optical technologies, integration nature's designs and modern engineering has paved way for groundbreaking innovations. Bio-inspired tunable optics photonics, drawing from intricate mechanisms found in biological systems, offer a new frontier adaptive efficient light management. Here, this review presents comprehensive examination principles, advancements, applications natural light-manipulation adaptation mechanisms, highlighting their translation into artificial photonic structures. Emphasizing remarkable potential bio-inspired particularly those emulating functionalities eyes skins, it explores current state devices. Our categorizes these systems two foundational mechanisms: light-adaptation, illustrating wide-ranging implications consumer electronics to next-generation technologies. This also highlights challenges prospects photonics. It emphasizes role promoting properties multifunctional devices, providing revolutionary opportunities across various sectors, including military everyday life, thus surpassing cutting-edge

Язык: Английский

---

Mechanism of Cone Degeneration in Retinitis Pigmentosa

Cellular and Molecular Neurobiology, Год журнала: 2022, Номер 43(3), С. 1037 - 1048

Опубликована: Июль 6, 2022

Язык: Английский

---

Embryonic mercury exposure in zebrafish: Alteration of metabolites and gene expression, related to visual and behavioral impairments

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 256, С. 114862 - 114862

Опубликована: Март 31, 2023

The widespread presence of mercury, a heavy metal found in the environment and used numerous industries domestic, raises concerns about its potential impact on human health. Nevertheless, adverse effects this environmental toxicant at low concentrations are often underestimated. There emerging studies showing that accumulation mercury eye may contribute to visual impairment comorbidity between autism spectrum disorders (ASD) trait impairment. However, underlying mechanism humans rodents is challenging. In response issue, zebrafish larvae with cone-dominated retinal system were exposed 100 nM chloride (HgCl2), according our previous study, followed by light-dark stimulation, social assay, color preference examine functionality relation ASD-like behavior. Exposure embryos HgCl2 from gastrulation hatching increased locomotor activity dark, reduced shoaling exploratory behavior, impaired preference. Defects microridges as first barrier serve primary tools for toxicity affecting vision. Depletion polyunsaturated fatty acids (PUFAs), linoleic acid, arachidonic acid (ARA), alpha-linoleic docosahexaenoic (DHA), stearic L-phenylalanine, isoleucine, L-lysine, N-acetylputrescine, along increase gamma-aminobutyric (GABA), sphingosine-1-phosphate, citrulline assayed liquid chromatography-mass spectrometry (LC-MS) suggest these metabolites biomarkers impairments affect vision Although suppression adsl, shank3a, tsc1b, nrxn1a gene expression was observed, among tsc1b showed more positive correlation ASD. Collectively, results new insights into possible give rise visual, cognitive, deficits zebrafish.

Язык: Английский

---

Rapid Assessment of Ocular Toxicity from Environmental Contaminants Based on Visually Mediated Zebrafish Behavior Studies

Toxics, Год журнала: 2023, Номер 11(8), С. 706 - 706

Опубликована: Авг. 16, 2023

The presence of contaminants in the environment has increased recent years, and studies have demonstrated that these ability to penetrate blood–retinal barrier directly affect visual systems organisms. Zebrafish are recognized as an ideal model for human eye diseases due their anatomical functional similarities eye, making them efficient versatile organism studying ocular toxicity caused by environmental field toxicology. Meanwhile, zebrafish exhibit a diverse repertoire visually mediated behaviors, system undergoes complex changes behavioral responses when exposed contaminants, enabling rapid assessment induced such pollutants. Therefore, this review aimed highlight effectiveness examining effects on development. Special attention is given behavior zebrafish, which allows resulting from exposure contaminants. Additionally, potential mechanisms may induce briefly outlined.

Язык: Английский

---

Targeting autophagy impairment improves the phenotype of a novel CLN8 zebrafish model

Neurobiology of Disease, Год журнала: 2024, Номер 197, С. 106536 - 106536

Опубликована: Май 17, 2024

CLN8 is an endoplasmic reticulum cargo receptor and a regulator of lysosome biogenesis whose loss function leads to neuronal ceroid lipofuscinosis. has been linked autophagy lipid metabolism, but much remains be learned, there are no therapies acting on the molecular signatures in this disorder. The present study aims characterize pathways involved disease and, by pinpointing altered ones, identify potential therapies. To bridge gap between cell mammalian models, we generated new zebrafish model deficiency, which recapitulates pathological features disease. We observed, for first time, that dysfunction impairs autophagy. Using modulators, showed trehalose SG2 able attenuate phenotype mutant larvae, confirming impairment as secondary event progression. Overall, our successful modeling defects highlights novel vivo model's strong instrument exploring role cellular pathways, with view identifying small molecules treat rare

Язык: Английский

---

Probing Photoreceptor Outer Segment Phagocytosis by the RPE In Vivo: Models and Methodologies

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(7), С. 3661 - 3661

Опубликована: Март 27, 2022

In the vertebrate retina, light-sensitive photoreceptor rods and cones constantly undergo renewal by generating new portions of outer segment shedding their distal, spent tips. The neighboring RPE provides critical function engulfing material phagocytosis. phagocytosis shed rod fragments is a circadian process that occurs in burst activity shortly after daily light onset with low at other times, rhythm has been reported for many species over 50 years. this review, we compare studies on quantity using different vivo model systems assessment methods. We discuss how measurement methodology impacts observation analysis Published investigating mice further suggest differences genetic background housing conditions may affect results. Altogether, comparison between performed differing strains same not as straightforward previously thought.

Язык: Английский

---