Pflügers Archiv - European Journal of Physiology, Год журнала: 2023, Номер 475(12), С. 1369 - 1373
Опубликована: Дек. 1, 2023
Язык: Английский
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Янв. 29, 2025
Abstract Identification of neuron type is critical to understand computation in neural circuits through extracellular recordings awake, behaving animal subjects. Yet, modern recording probes have limited power resolve type. Here, we leverage the well-characterized architecture cerebellar circuit perform expert identification from non-human primates. Using deep-learning classifiers evaluate information contained readily accessible features for identification. Waveform, discharge statistics, anatomical layer, and functional interactions each can inform labels a sizable fraction units. Together, as inputs classifier, even better. Our tools methodologies, validated during smooth pursuit eye movements floccular complex awake monkeys, guide cerebellar-dependent tasks animals across species. They lay groundwork characterization processing cortex. Impact statement To how brain performs computations service behavior, develop methods link activity within circuits. show derived provide complementary disambiguate identity
Язык: Английский
Процитировано
1
Physiological Measurement, Год журнала: 2024, Номер 45(6), С. 065001 - 065001
Опубликована: Май 21, 2024
Temperature plays a crucial role in influencing the spatiotemporal dynamics of heart. Electrical instabilities due to specific thermal conditions typically lead early period-doubling bifurcations and beat-to-beat alternans. These pro-arrhythmic phenomena manifest voltage calcium traces, resulting compromised contractile behaviors. In such intricate scenario, dual optical mapping technique was used uncover unexplored multi-scale nonlinear couplings, essential for detection understanding cardiac arrhythmia.
Язык: Английский
Процитировано
2
Communications Biology, Год журнала: 2024, Номер 7(1)
Опубликована: Авг. 27, 2024
In order to facilitate cardiovascular research develop non-invasive optical heart pacing methods, we have generated a double-transgenic Drosophila melanogaster (fruit fly) model suitable for optogenetic pacing. We created fly stock with both excitatory H134R-ChR2 and inhibitory eNpHR2.0 opsin transgenes. Opsins were expressed in the using Hand-GAL4 driver. Here describe Hand > H134R-ChR2; characterization including bi-directional control (activation inhibition) upon illumination of light distinct wavelengths. Optical real-time visualization function achieved non-invasively an integrated stimulation coherence microscopy (OCM) system. OCM produced high-speed high-resolution imaging; simultaneously, was modulated by blue (470 nm) or red (617 pulses causing tachycardia, bradycardia restorable cardiac arrest episodes same animal. The irradiance power levels schedules optimized achieve successful larvae pupae. beating
Язык: Английский
Процитировано
2
Journal of Molecular and Cellular Cardiology, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Дек. 17, 2024
Abstract Bioelectricity is a fundamental biophysical phenomenon present in all cells, playing crucial role embryogenesis by regulating processes such as neuronal signaling, pattern formation, and cancer suppression. Precise monitoring of bioelectric signals their dynamic changes throughout development vital for advancing our understanding higher organisms. However, the lack suitable techniques mapping during early has greatly limited ability to interpret these mechanisms. To address this challenge, we developed an Ace2N-mNeon expression library zebrafish, which exhibits membrane localization from 4 hours post-fertilization at least 5 days post- fertilization broad across multiple cell types development. We validated use studying via voltage imaging record neurons cardiomyocytes different stages. Through approach, found evidence synchronized activity observed faster dynamics progressed. Our results show that valuable tool developmental studies supporting advanced fluorescence lifetime (FLIM). These methods enable non-invasive, diverse development, significantly surpassing capabilities current electrophysiological techniques.
Язык: Английский
Процитировано
0
Colorants, Год журнала: 2024, Номер 3(4), С. 360 - 377
Опубликована: Дек. 23, 2024
Advancements in cellular imaging have significantly enhanced our understanding of membrane potential and Ca2⁺ dynamics, which are crucial for various processes. Voltage-sensitive dyes (VSDs) pivotal this field, enabling non-invasive, high-resolution visualization electrical activity cells. This review discusses the types VSDs, including electrochromic, Förster Resonance Energy Transfer (FRET)-based, Photoinduced Electron (PeT)-based dyes. VSDs essential tools studying mitochondrial neuronal function frequently used conjunction with indicators to elucidate complex relationship between fluxes. The development novel improved photostability reduced toxicity continues expand biomedical research. underscores importance advancing bioenergetics, signaling, disease mechanisms.
Язык: Английский
Процитировано
0
Pflügers Archiv - European Journal of Physiology, Год журнала: 2023, Номер 475(12), С. 1369 - 1373
Опубликована: Дек. 1, 2023
Язык: Английский
Процитировано
0