From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches

Metabolites, Год журнала: 2023, Номер 13(8), С. 895 - 895

Опубликована: Июль 28, 2023

Secondary metabolites are gaining an increasing importance in various industries, such as pharmaceuticals, dyes, and food, is the need for reliable efficient methods of procuring these compounds. To develop sustainable cost-effective approaches, a comprehensive understanding biosynthetic pathways factors influencing secondary metabolite production essential. These compounds unique type natural product which recognizes oxidative damage caused by stresses, thereby activating defence mechanism plants. Various have been developed to enhance The elicitor-induced vitro culture technique considered tool studying improving In present review, we documented role under diverse environmental stresses. Furthermore, practical strategy obtaining consistent abundant via elicitation agents used culturing techniques also mentioned. By elucidating intricate interplay regulatory factors, this review paves way future advancements high-value metabolites.

Язык: Английский

---

Unveiling characteristic metabolic accumulation over enzymatic-catalyzed process of Tieguanyin oolong tea manufacturing by DESI-MSI and multiple-omics

Food Research International, Год журнала: 2024, Номер 181, С. 114136 - 114136

Опубликована: Фев. 19, 2024

Язык: Английский

---

An integrated metabolomic and transcriptomic analysis reveals the dynamic changes of key metabolites and flavor formation over Tieguanyin oolong tea production

Food Chemistry X, Год журнала: 2023, Номер 20, С. 100952 - 100952

Опубликована: Окт. 21, 2023

To interpret the formation characteristic flavor during oolong tea manufacturing process, dynamic changes of key components in samples from various processing steps Tieguanyin production were investigated using widely-targeted metabolomic and transcriptomic approaches. As a result, total 1078 metabolites determined, which 62 compounds identified as biomarkers significantly changed over process. Quantitative determination 50,343 transcripts showed 7480 them co-expressed different genes. Glutamic acid served critical metabolism hub signaling molecule for diverse stress responses. Additionally, targeted quantification results that contents catechins xanthine alkaloids dried dramatically decreased by 20.19% 7.15% respectively than those fresh leaves, potentially contributed to alleviation astringent or bitter palates, promoting mellow rich tea.

Язык: Английский

---

Alfalfa Responses to Intensive Soil Compaction: Effects on Plant and Root Growth, Phytohormones and Internal Gene Expression

Plants, Год журнала: 2024, Номер 13(7), С. 953 - 953

Опубликована: Март 26, 2024

The perennial legume alfalfa (Medicago sativa L.) is of high value in providing cheap and high-nutritive forages. Due to a lack tillage during the production period, soil which grows prunes become compacted through highly mechanized agriculture. Compaction deteriorates soil’s structure fertility, leading compromised development productivity. However, way responses different levels compaction underlying molecular mechanism are still unclear. In this study, we systematically evaluated effects gradient on growth cultivars alfalfa, especially root system architecture, phytohormones internal gene expression profile alterations. results showed that was facilitated by moderate compaction, but drastically inhibited when intensified. inhibition effect universal across cultivars, with severity. Transcriptomic physiological studies revealed set genes regulating biosynthesis lignin flavonoids significantly repressed treated roots, might have resulted modified secondary cell wall xylem vessel formation. Phytohormones, like ABA, supposed play pivotal roles regulation overall responses. These findings provide directions for improvement field management breeding germplasm better resilience.

Язык: Английский

---

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Physiologia Plantarum, Год журнала: 2024, Номер 176(2)

Опубликована: Март 1, 2024

Abstract Plant growth is restricted by salt stress, which a significant abiotic factor, particularly during the seedling stage. The aim of this study was to investigate mechanisms underlying peanut adaptation stress transcriptomic and metabolomic analysis In study, phenotypic variations FH23 NH5, two varieties with contrasting tolerance salt, changed obviously, strongest differences observed at 24 h. leaves wilted membrane system seriously damaged. A total 1470 metabolites were identified, flavonoids being most common (21.22%). Multi‐omics analyses demonstrated that flavonoid biosynthesis (ko00941), isoflavones (ko00943), plant hormone signal transduction (ko04075) key metabolic pathways. comparison in isoflavone pathways different tolerant levels accumulation naringenin formononetin may be metabolite leading their tolerance. Using our data, we identified three possible reasons for difference between varieties: (1) differential expression LOC112715558 ( HIDH ) LOC112709716 HCT ), (2) LOC112719763 PYR/PYL LOC112764051 ABF abscisic acid (ABA) pathway, then (3) genes encoding JAZ proteins LOC112696383 LOC112790545 ). Key candidate related improving peanuts screened promote responses NaCl guide genetic improvement.

Язык: Английский

---

Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots under Lead Stress

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(11), С. 6050 - 6050

Опубликована: Май 31, 2024

Flavonoids are secondary metabolites that play important roles in the resistance of plants to abiotic stress. Despite widely reported adverse effects lead (Pb) contamination on maize, Pb biosynthetic processes flavonoids maize roots still unknown. In present work, we employed a combination multi-omics and conventional assay methods investigate two concentrations (40 250 mg/kg) flavonoid biosynthesis associated molecular regulatory mechanisms. Analysis using assays revealed 40 mg/kg exposure increased content root 0.67 ± 0.18 3.09 0.02 mg/kg, respectively, but they did not result significant changes length. The results suggested caused differential expression 33 genes 34 related system, while 31 metabolites. Not only these differentially expressed participate transferase activity, anthocyanin-containing compound processes, metal ion binding, hydroxyl group cinnamoyl hydroxycinnamoyl flavanone 4-reductase activity were also significantly enriched flavonoid, isoflavonoid, flavone, flavonol pathways. These show is involved regulation growth by interfering with system. this study will enable elucidation mechanisms systems.

Язык: Английский

---

Combined transcriptional and metabolomic analysis of flavonoids in the regulation of female flower bud differentiation in Juglans sigillata Dode

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 10, 2025

Juglans sigillata Dode is rich in flavonoids, but the low ratio of female to male flower buds limits development J. industry. While abundance flavonoids known, whether influence bud differentiation has not been reported. In this study, we explored regulatory mechanisms gene expression and metabolite accumulation during through integrated transcriptomic metabolomic analyses. Our findings revealed that flavonoid biosynthesis a key pathway influencing differentiation, with metabolites primarily shifting towards isoflavonoid, flavone, flavonol branches. Structural genes such as chalcone synthase, dihydroflavonol 4-reductase, 3',5'-hydroxylase were identified playing crucial roles. The these promoted which turn influenced by modulating including Suppressor Overexpression Constans1, Constans, Flowering Locus T, APETALA1. Furthermore, transcription factors (TFs) highly expressed physiological buds, particularly M-type MADS, WRKY, MYB, positively correlated genes, indicating their significant role regulation production. These results offer valuable insights into highlight plant differentiation.

Язык: Английский

---

Waterlogging alone and combined with other abiotic stresses provides unique metabolic signatures at the plant-rhizosphere interface: a multi-omics perspective on root metabolome, root exudation and rhizomicrobiome

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 221, С. 109646 - 109646

Опубликована: Фев. 12, 2025

Despite the growing evidence on unique and unpredictable impact of stress combination over plants, waterlogging-combined stresses effects are still underexplored. Under those conditions, besides impairment plant aerial parts, root system is particularly vulnerable, leading to consequences survival. Here, we report short-term exposure soil-grown Arabidopsis thaliana L. waterlogging alone combined with cold, heat, salinity inspect their antagonistic, additive or synergistic in rhizosphere. To this aim, metabolic changes, exudation profiles, microbial diversity were investigated using a metabolomics metagenomics, interaction was analysed through multi-omics data integration. In roots, strongly affected metabolism compared other single stresses, causing down-accumulation targeted classes compounds including, phenylpropanoids, sterols, terpenoids, alkaloids. Additive reported roots under heat cold respectively. Regarding exudates, flavonoids, alkaloids main affected. Waterlogging caused all except for coumarins, mixed trends observed waterlogging-salinity resulting an ameliorating effect. Even though communities' alpha- beta-diversity remained stable, suggesting resilience exposure, specific taxa modulation recorded each condition. Overall, these results contribute understanding hierarchical exudation, influencing rhizosphere interactions. This approach advances our responses dynamics, paving way future studies adaptive mechanisms.

Язык: Английский

---

Metabolomic analyses reveal that graphene oxide alleviates nicosulfuron toxicity in sweet corn

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Фев. 25, 2025

Nicosulfuron can repress the growth and quality of sweet corn (Zea mays), graphene oxide has been used for sustainable agriculture. However, underlying mechanism toxicity nicosulfuron that is mediated in remains elusive. To explore potential GO-mediated this study, we investigated effects on stress sister inbred lines H01 H20. Furthermore, performed a metabolomics analysis H20 under different treatments. The results showed severely affected rate survival, physiological parameters, photosynthetic indicators, chlorophyll fluorescence parameters seedlings, whereas foliar spraying with promoted survival toxicity. 70 90 metabolites differentially accumulated treatment, respectively. Graphene restored 59 seedlings 56 to normal levels thereby promoting seedlings. Compared treatment alone, resulted 108 66 differential lines, A correlation revealed metabolites, such as doronine (R)-2-hydroxy-2-hydroxylase-1,4-benzoxazin-3(4-hydroxylase)-1, were significantly correlated parameters. related detoxification enriched flavonoid metabolic pathways. These collectively indicate be regulator provide insights into their use improve crops areas are contaminated nicosulfuron.

Язык: Английский

---

Environmental Stress Induced Biosynthesis of Plant Secondary Metabolites- Transcriptional Regulation as a key

Crop Design, Год журнала: 2025, Номер unknown, С. 100100 - 100100

Опубликована: Фев. 1, 2025

Язык: Английский

---