Alternative splicing and related RNA binding proteins in human health and disease
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Фев. 2, 2024
Abstract
Alternative
splicing
(AS)
serves
as
a
pivotal
mechanism
in
transcriptional
regulation,
engendering
transcript
diversity,
and
modifications
protein
structure
functionality.
Across
varying
tissues,
developmental
stages,
or
under
specific
conditions,
AS
gives
rise
to
distinct
splice
isoforms.
This
implies
that
these
isoforms
possess
unique
temporal
spatial
roles,
thereby
associating
with
standard
biological
activities
diseases.
Among
these,
AS-related
RNA-binding
proteins
(RBPs)
play
an
instrumental
role
regulating
alternative
events.
Under
physiological
the
diversity
of
mediated
by
influences
structure,
function,
interaction,
localization
proteins,
participating
differentiation
development
array
tissues
organs.
pathological
alterations
are
linked
various
diseases,
particularly
cancer.
These
changes
can
lead
gene
patterns,
culminating
loss
For
instance,
cancer,
abnormalities
RBPs
may
result
aberrant
expression
cancer-associated
genes,
promoting
onset
progression
tumors.
also
associated
numerous
neurodegenerative
diseases
autoimmune
Consequently,
study
across
different
holds
significant
value.
review
provides
detailed
account
recent
advancements
tissue
which
aids
deepening
understanding
complexity
offers
new
insights
methodologies
for
precision
medicine.
Язык: Английский
The pineapple reference genome: Telomere‐to‐telomere assembly, manually curated annotation, and comparative analysis
Journal of Integrative Plant Biology,
Год журнала:
2024,
Номер
66(10), С. 2208 - 2225
Опубликована: Авг. 7, 2024
ABSTRACT
Pineapple
is
the
third
most
crucial
tropical
fruit
worldwide
and
available
in
five
varieties.
Genomes
of
different
pineapple
varieties
have
been
released
to
date;
however,
none
them
are
complete,
with
all
exhibiting
substantial
gaps
representing
only
two
This
significantly
hinders
advancement
breeding
efforts.
In
this
study,
we
sequenced
genomes
three
varieties:
a
wild
variety,
fiber
globally
cultivated
edible
variety.
We
constructed
first
gap‐free
reference
genome
(Ref)
for
pineapple.
By
consolidating
multiple
sources
evidence
manually
revising
each
gene
structure
annotation,
identified
26,656
protein‐coding
genes.
The
BUSCO
evaluation
indicated
completeness
99.2%,
demonstrating
high
quality
annotations
genome.
Utilizing
these
resources,
7,209
structural
variations
across
Approximately
30.8%
genes
were
located
within
±5
kb
variations,
including
30
associated
anthocyanin
synthesis.
Further
analysis
functional
experiments
demonstrated
that
expression
AcMYB528
aligns
accumulation
anthocyanins
leaves,
both
which
may
be
affected
by
1.9‐kb
insertion
fragment.
addition,
developed
Ananas
Genome
Database,
offers
data
browsing,
retrieval,
analysis,
download
functions.
construction
database
addresses
lack
resource
databases.
summary,
acquired
seamless
high‐quality
annotations,
providing
solid
foundation
genomics
valuable
breeding.
Язык: Английский
The golden genome annotation of Ganoderma lingzhi reveals a more complex scenario of eukaryotic gene structure and transcription activity
BMC Biology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 25, 2024
It
is
generally
accepted
that
nuclear
genes
in
eukaryotes
are
located
independently
on
chromosomes
and
expressed
a
monocistronic
manner.
However,
accumulating
evidence
suggests
more
complex
landscape
of
gene
structure
transcription.
Ganoderma
lingzhi,
model
medicinal
fungus,
currently
lacks
high-quality
genome
annotation,
hindering
genetic
studies.
Язык: Английский
Differential induction of defense genes in hexaploid wheat roots by the plant-parasitic nematodes Pratylenchus neglectus and P. thornei
PLoS ONE,
Год журнала:
2024,
Номер
19(8), С. e0306533 - e0306533
Опубликована: Авг. 29, 2024
Pratylenchus
neglectus
and
P.
thornei
are
among
the
most
destructive
root
lesion
nematodes
of
wheat
in
Pacific
Northwest,
United
States
America
throughout
world.
The
aim
this
study
was
to
determine
whether
both
nematode
species
were
similar
their
ability
induce
defense
genes
roots
genotype
Scarlet,
a
combination
induced
different
pattern
gene
induction
than
each
alone.
long-term
aspect
research
identify
nematode-inducible
promoters
for
deploying
breeding
programs.
transcriptomes
Scarlet
obtained
after
one-week
infection
period
with
separately,
or
combined.
Root
expression
all
three
treatments
relative
no-nematode
control,
but
affected
greater
extent
compared
neglectus.
highest
number
genes.
This
result
not
predicted
from
enumeration
studies,
which
colonization
substantially
lower
that
neglectus,
did
show
significant
difference.
Quantitative
real
time
polymerase
chain
reaction
(qRT-PCR)
assays
Dehydrin2,
Glucan
endo-1,3-beta-glucosidase,
1-cys-Peroxiredoxin,
Pathogenesis-related
protein
1
Late
embryogenesis-abundant
proteins
76
group
3
authenticated
observed
transcriptome
data.
In
addition,
near-isogenic
line
harboring
genetic
resistance
fungal
soilborne
pathogens,
called
Scarlet-Rz1,
showed
higher
levels
fungus-susceptible
qRT-PCR
assays.
Finally,
patterns
revealed
responsive
thornei.
Язык: Английский