Bioengineering, Год журнала: 2024, Номер 11(11), С. 1135 - 1135
Опубликована: Ноя. 11, 2024
Non-unions are fractures that do not heal properly, resulting in a false joint formation at the fracture site. This condition leads to major health issues and imposes burden on national healthcare systems. The etiology of non-unions is still fully understood; therefore, we aimed identify potential systemic factors may contribute their formation.
Язык: Английский
Frontiers in Oncology, Год журнала: 2024, Номер 14
Опубликована: Сен. 5, 2024
Papillary thyroid carcinoma (PTC) is the most common malignancy, and its distant metastasis (PTCDM), although uncommon, seriously affects survival rate quality of life patients. With rapid development science technology, research in field PTCDM has accumulated rapidly, presenting a complex knowledge structure trend.
Язык: Английский
Процитировано
1
Journal of Proteome Research, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 26, 2024
Patients with lateral lymph node metastasis (LLNM) may experience higher locoregional recurrence rates and poorer prognoses compared to those without LLNM, highlighting the need for effective preoperative stratification reliably assess risk LLNM. In this study, we collected PTMC samples from Peking Union Medical College Hospital employed data-independent acquisition mass spectrometry proteomics technique identify protein profiles in tissues Pseudo temporal analysis single sample gene set enrichment were conducted combination The Cancer Genome Atlas Thyroid Carcinoma functional coordination construction of a prediction model based on random forest. Non-negative matrix factorization (NMF) clustering was utilized classify molecular subtypes PTMC. Our findings revealed that differential activation pathways such as MAPK PI3K critical enhancing metastatic potential We successfully screened biomarkers via machine learning public databases, creating an metastasis. Additionally, explored mechanism metastasis-associated NMF clustering. These insights into LLNM mechanisms contribute future biomarker screening identification therapeutic targets.
Язык: Английский
Процитировано
1
Biomedicines, Год журнала: 2024, Номер 12(1), С. 136 - 136
Опубликована: Янв. 9, 2024
Recent research has revealed the importance of miRNAs in diagnosis and clinical evolution papillary thyroid cancer (PTC). We aim to identify a specific miRNA profile that could differentiate between subtypes PTC. In this cross-sectional study, total RNA was extracted from paraffin-embedded tissues 43 patients, 17 with an infiltrative follicular variant PTC (iFVPTC) 26 conventional (cPTC). Nine were evaluated using qRT-PCR technology assays. found patterns for cPTC iFVPTC, such as altered both types tumours (miR-146b-5p, miR-181a-5p, miR-221-3p, miR-21-5p miR-222-3p) two significantly expressed only (miR-20b-5p, miR-21-5p). The iFVPTC group presented strong moderate correlations expression data. miR-195-5p, miR-181-5p, miR-146b-5p miR-222 correlated age, tumour size (TS) or lymph node metastases (N), while miR-20b-5p, miR-195-5p miR-181-5p TS, N age group. present study allowed identification signature confirm differences histological TC. Our results provide advances molecular TC help improve diagnostic performance already existing classifiers.
Язык: Английский
Процитировано
0
Journal of Proteomics, Год журнала: 2024, Номер 306, С. 105260 - 105260
Опубликована: Июль 17, 2024
Thyroid cancer has emerged as the most rapidly proliferating solid neoplasm. In this study, we included a cohort of patients who underwent sonographic assessment and surgical intervention at Sir Run Shaw Hospital, associated with School Medicine Zhejiang University, spanning from January 2019 to June 2020. Stratification cases was based on combination preoperative ultrasonographic evaluations postoperative histopathological diagnoses, resulting in three distinct groups: high-risk papillary thyroid carcinoma (PTC) labeled C1, low-risk PTC designated C2, control group (N) composed benign tissue adjacent carcinoma. Proteomic phosphoproteomic analyses were conducted specimens. The comparative revealed that proteins up-regulated C1/N C2/N groups predominantly involved functions such amino acid binding, binding phosphorylated compounds, serine protease activity. Notably, like NADH dehydrogenase, ATP synthase, oxidoreductases, iron ion channels significantly elevated C1 versus C2 group. Through meticulous analysis differential expression multiples, statistical significance, involvement metabolic pathways, study identified eight potential biomarkers pertinent metastasis diagnostics, encompassing myosin 10, proline-directed protein kinase, leucine tRNA synthetase, 2-oxo-isovalerate succinic semialdehyde ADP/ATPtranslocase, pyruvate carboxylase, fibrinogen. Therapeutic assays employing metformin, an AMP-activated kinase (AMPK) activator, alongside phosphorylation-specific inhibitor ML-7 targeting Myosin10, demonstrated attenuated cellular proliferation, migration, invasion capabilities cells, accompanied by reduction pools. Cellular colocalization interaction studies elucidated AMPK activation imposes inhibitory influence Myosin10 levels. findings research corroborate utility proteomic platforms identification metastatic markers for suggest modulation activity, coupled inhibition phosphorylation, may forge novel therapeutic avenues management SIGNIFICANCE: significance our lies its transform current understanding (PTC), particularly form. By integrating both analyses, not only sheds light molecular alterations but also identifies could serve indicators potential.
Язык: Английский
Процитировано
0
Medical Oncology, Год журнала: 2024, Номер 41(12)
Опубликована: Ноя. 5, 2024
Язык: Английский
Процитировано
0
Bioengineering, Год журнала: 2024, Номер 11(11), С. 1135 - 1135
Опубликована: Ноя. 11, 2024
Non-unions are fractures that do not heal properly, resulting in a false joint formation at the fracture site. This condition leads to major health issues and imposes burden on national healthcare systems. The etiology of non-unions is still fully understood; therefore, we aimed identify potential systemic factors may contribute their formation.
Язык: Английский
Процитировано
0