Characterization and evaluation of the cytotoxic, antioxidant, and anti-human lung cancer properties of copper nanoparticles green-synthesized by fennel extract following the PI3K/AKT/Mtor signaling pathway

PLoS ONE, Год журнала: 2025, Номер 20(1), С. e0309207 - e0309207

Опубликована: Янв. 9, 2025

This work established the cytotoxic, antioxidant and anticancer effects of copper nanoparticles (CuNPs) manufactured with fennel extract, especially on non-small cell lung cancer (NSCLC) as well. CuNPs caused cytotoxicity in a dose-dependent manner for two NSCLC lines, A549 H1650. At 100 μg/ml, reduced viability to 70% cells 65% H1650 cells. which showed cytotoxic effect (p<0. 05). Lactate dehydrogenase (LDH) was correspondingly present high proportion cells, demonstrated upon testing. Together their properties, antioxidative activity. When concentration nano particles (100 μg/ml), ratio reactive oxygen species (ROS) much 50%, turn suggested There plenty evidence that had anti-cancer potential; this has been shown by molecules PI3K/AKT/mTOR pathway, one pathways crucial survival. Western blot analysis qRT-PCR results indicated widespread degradation proteins pathway CuNP exposure. Interestingly, there declined phosphorylation up 75% PI3K, AKT, mTOR at μg/ml 001). In summary, these findings illustrated mechanisms behind therapeutic CuNPs, thus making them good targets treatment. have capacity, well significant alterations cancers therefore they can be considered candidates.

Язык: Английский

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Ability of SPP1 to Alleviate Post‐Intracerebral Hemorrhage Ferroptosis via Nrf2/HO1 Pathway

Brain and Behavior, Год журнала: 2025, Номер 15(5)

Опубликована: Май 1, 2025

ABSTRACT Purpose This study aimed to investigate the role of secretory phosphoprotein 1 (SPP1/OPN) in modulating iron‐induced cell death (ferroptosis) following intracerebral hemorrhage (ICH). By integrating transcriptomic analysis and experimental validation, we sought identify key molecular pathways therapeutic targets associated with ferroptosis ICH. Method The Gene Expression Omnibus Series GSE24265 dataset was analyzed using limma package (R platform) differentially expressed genes. Kyoto Encyclopedia Genes Genomes (KEGG) pathway Ontology (GO) enrichment analyses were performed elucidate biological functions. mortality identified by cross‐referencing profiles FerrDb database. A protein–protein interaction network constructed Cytoscape, hub genes identified. An ICH model developed mice stereotactic instrumentation, effects OPN administration evaluated through neurological assessments, biochemical assays (superoxide dismutase, glutathione, malondialdehyde), Western immunoblotting (GPX4, ACSL4), Prussian blue histochemistry, electron microscopy. Finding Transcriptomic 27 genes, CD44 ITGB3 characterized as receptors for OPN. In model, improved outcomes, elevated antioxidant markers, reduced lipid peroxidation. upregulated GPX4 while suppressing ACSL4, indicating anti‐ferroptotic effects. These protective mediated Nrf2 pathway, confirmed inhibitor ML385. staining microscopy demonstrated cerebral iron deposition mitochondrial damage treatment. Conclusion provides novel evidence SPP1/OPN a modulator ICH, highlighting its potential target. enhancing homeostasis mitigating oxidative stress, offers promising strategy improving outcomes patients.

Язык: Английский

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Treatment strategies targeting the phosphoinositide 3-kinase/protein kinase B/mechanistic target of rapamycin pathway against triple-negative breast cancer

World Journal of Clinical Oncology, Год журнала: 2025, Номер 16(5)

Опубликована: Май 19, 2025

Triple negative breast cancer (TNBC) is an exceptionally aggressive subtype of with a poor prognosis. TNBC patients have limited treatment options beyond conventional chemotherapy, and they face significant challenges associated disease recurrence resistance to chemotherapy. The phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mechanistic target rapamycin (mTOR) signaling pathway plays pivotal role in cell proliferation, growth, metabolism, survival. Its aberrant activation closely linked the development progression TNBC, as well response drug resistance. Currently, numerous targeted drugs specifically inhibiting this are being developed undergoing clinical trials. These include inhibitors targeting PI3K, AKT, or mTOR individually, dual-target multi-target simultaneously different components pathway. Encouragingly, some demonstrated promising potential This review delves into therapeutic PI3K/AKT/mTOR for explores prospects discovery.

Язык: Английский

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Double-Edged Sword Effect of Diet and Nutrition on Carcinogenic Molecular Pathways in Breast Cancer

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11078 - 11078

Опубликована: Окт. 15, 2024

Environmental exposure to a mixture of chemical xenobiotics acts as double-edged sword, promoting or suppressing tumorigenesis and the development breast cancer (BC). Before anything else, we are what eat. In this review, highlight both “the good” bad” sides daily human diet dietary patterns that could influence BC risk (BCR) incidence. Thus, regularly eating new, diversified, colorful, clean, nutrient-rich, energy-boosting, raw food, increases apoptosis autophagy, antioxidation, cell cycle arrest, anti-inflammation, immune response against cells. Moreover, healthy lead reduction in inhibition genomic instability, stemness, growth, proliferation, invasion, migration, distant metastasis. We also emphasize that, addition beneficial compounds, our food is more contaminated by chemicals with harmful effects, which interact each other endogenous proteins lipids, resulting synergistic antagonistic effects. diverse diet, combined appropriate nutritional behaviors, can exert anti-carcinogenic effects improve treatment efficacy, patient outcomes, overall quality life patients.

Язык: Английский

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