Supplemental Description of Caballerotrema annulatum (Diesing, 1850) Ostrowski de Núñez and Sattmann, 2002 (Digenea: Caballerotrematidae) from a New Host (Electrophorus Cf. Varii) and Locality (Amazon River, Colombia) with Phylogenetic Analysis and Emended Generic Diagnosis

Journal of Parasitology, Год журнала: 2024, Номер 110(4)

Опубликована: Июль 10, 2024

Herein, we provide a supplemental description of Caballerotrema annulatum (Diesing, 1850) Ostrowski de Núñez and Sattmann, 2002 (Digenea: Caballerotrematidae Tkach, Kudlai, Kostadinova, 2016) based on specimens collected from the intestine an electric eel, Electrophorus cf. varii (Gymnotiformes: Gymnotidae) captured in Amazon River (Colombia). This caballerotrematid can be differentiated its congeners by following combination morphological features: body surface spines forming contiguous transverse rows, concentric (wrapping dorso-ventrally around body), distributing into posterior half (vs. restricted to anterior brasiliensePrudhoe, 1960; indeterminate for aruanenseThatcher, 1980 piscicola [Stunkard, 1960] Kostadinova Gibson, 2001); head collar lacking projections having them C. brasiliense, aruanense, piscicola), narrow (head more than maximum width vs. being obviously wider piscicola); corner clustered as 2 separated pairs pharynx approximately at level far testes ovoid nonoverlapping (C. aruanense; sinuous overlapping brasiliense piscicola). Based our results, revise diagnosis CaballerotremaPrudhoe, 1960 include features associated with shape distribution spines, orientation position cirrus sac, seminal vesicle, oviduct, Laurer's canal, oötype, vitellarium, vitelline ducts. We performed Bayesian inference analyses using partial large subunit ribosomal (28S) DNA gene. Our 28S sequence was recovered sister that sp. (which is only other available GenBank) arapaima, Arapaima gigas (Schinz, 1822) (Osteoglossiformes: Arapaimidae) Peruvian Amazon. comprises sequenced tethered voucher specimen lending museum. The present study new host record locality annulatum. phylogeny most resolved taxon-rich evolutionary hypothesis Echinostomatoidea published date.

Язык: Английский

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Overview of renicolid digeneans (Digenea, Renicolidae) from marine gulls of northern Holarctic with remarks on their species statuses, phylogeny and phylogeography

Parasitology, Год журнала: 2022, Номер 150(1), С. 55 - 77

Опубликована: Ноя. 2, 2022

Abstract Renicolid digeneans parasitize aquatic birds. Their intramolluscan stages develop in marine and brackish-water gastropods, while metacercariae molluscs fishes. The systematics of renicolids is poorly developed, their life cycles are mostly unknown, the statuses many species require revision. Here, we establish based on integrated morphological molecular data that adult from gulls Larus argentatus schistisagus sporocysts cercariae Cercaria parvicaudata snails Littorina spp. life-cycle same species. We name it Renicola parvicaudatus synonymized with roscovitus . An analysis cox1 gene R. Europe, North America Asia demonstrates a low genetic divergence, suggesting this has formed quite recently (perhaps during last glacial maximum) interregional flow high. In saxatilis L. obtusata Barents Sea, revealed littorinae VIII, cryptic relative to trees, keimahuri belongs another clade than show have Rhodometopa group outline behavioural transformations leading xiphidiocercariae these larvae. Molecular 3 main phylogenetic branches renicolids, differing structure adults, type host range. Our results elucidate patterns colonization geographical expansion pave way solution some long-standing problems classification.

Язык: Английский

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Untangling the “Renicola somateria” (Digenea, Renicolidae) Muddle: Actual Number of Species and Their Distribution and Transmission in the Holarctic

Diversity, Год журнала: 2024, Номер 16(7), С. 402 - 402

Опубликована: Июль 12, 2024

Renicolids are parasites of aquatic birds. Their species identification based on morphological characters is problematic. Here, we revised the composition Renicola spp. parasitising anatids in nearshore areas northern seas using integrated and molecular data. We redescribed somateria verified diagnosis R. mediovitellata. established that first intermediate host (FIH) mollusc Buccinum undatum, while FIHs mediovitellata Nucella molluscs. described intramolluscan stages both species. formed a separate clade trees Renicolidae. This finding confirms existence three main phylogenetic branches renicolids, differing structure adults, type cercariae, range. Molecular data demonstrated an amphiboreal distribution The former represented by single population Europe North Pacific, latter forms populations these regions. may be because actually uses not only B. undatum but also some other buccinid with similar circum-Arctic ranges as FIH. discuss roles played formation digenean vagility definitive host, lifespan

Язык: Английский

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Elucidating the life cycle of opossum parasites: DNA sequences reveal the involvement of planorbid snails as intermediate hosts of Rhopalias spp. (Trematoda: Echinostomatidae) in Brazil

PLoS ONE, Год журнала: 2023, Номер 18(3), С. e0279268 - e0279268

Опубликована: Март 3, 2023

Echinostomatid digeneans belonging to the genus Rhopalias are intestinal trematodes found mainly in opossums New World. The comprises seven species, but their life cycles and intermediate hosts have been unknown until now. During our long-term study carried out freshwater habitats within state of Minas Gerais, Southeast Brazil, echinostomatid cercariae lacking collar spines were planorbid snails Biomphalaria glabrata, straminea, Drepanotrema lucidum Gundlachia ticaga six different batches snail samples collected between 2010 2019. Morphologically, larvae reported herein morphologically consistent with each other characterized by presence 2-3 large ovoid or spherical corpuscles main duct excretory system, resembling Cercaria macrogranulosa previously described from same region Brazil. Partial sequences ITS (ITS1-5.8S-ITS2) 28S gene nuclear ribosomal RNA operon, partial mitochondrial nad1 cox1 genes obtained compared data available for members family Echinostomatidae. Nuclear markers indicate that all evaluated present can be assigned Rhopalias, distinct North American isolates macracanthus, coronatus oochi (divergence 0.2-1.2% 0.8-4.7% ITS). lack differences verified both 5 6 studied suggested they belong species. However, revealed correspond three species (interspecific divergence: 7.7-9.9%), named here as sp. 1, B. straminea G. ticaga, 2 glabrata D. lucidum, 3 also lucidum. They differ 10.8-17.2% a isolate R. macracanthus sequenced this study. 1 (but not 3) reveal (genetic divergence 16.3-16.5% 15.6-15.7%, respectively), (9.2-9.3% 9.3-9.5%) (9.0% 9.5-10.1%). Encysted metacercariae general morphology similar body tadpoles Rhinella stream where harbored 2, suggesting amphibians could act second Rhopalias. Data provide first insights into cycle unusual genus.

Язык: Английский

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Himasthla spp. (Trematoda) in the edible cockle Cerastoderma edule: review, long-term monitoring and new molecular insights

Parasitology, Год журнала: 2022, Номер 149(7), С. 878 - 892

Опубликована: Март 30, 2022

Abstract Trematodes are the main macroparasites in coastal waters. The most abundant and widespread form of these parasites is metacercaria. Their impact on their host fitness considered relatively low but metacercarial larvae some species can have deleterious effects individuals and/or populations. This review focused cockle Cerastoderma edule four genus Himasthla ; a common host–parasite system marine environments. Our aims were (1) to literature concerning continua , elongata interrupta quissetensis cockles; (2) provide molecular signatures (3) analyse infection patterns using 20-year monthly database monitoring from Banc d'Arguin (France). Due identification uncertainties, analysis was restricted H. it revealed that infect cockles same size range. intensity increased with size/age. During colder months, mean parasite cohort decreased, while occurred warmest season. No inter-specific competition between trematode detected. Furthermore, even if or fluctuated different years, this did not modify community structure cockles. both also positively correlated richness abundance. study highlighted possible detrimental role spp. population dynamics. It risks misidentification, which should be resolved by further approaches.

Язык: Английский

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A month for the mission: using a sentinel approach to determine the transmission window of digenean cercariae in the subarctic White Sea

Journal of Helminthology, Год журнала: 2021, Номер 95

Опубликована: Янв. 1, 2021

In the digenean life cycle cercaria ensures an important transmission stage, from first intermediate host to second or definitive host. regions with pronounced seasonality, this process occurs within a certain interval, window. high latitudes, size of window has previously been determined only by comparing data on seasonal dynamics infection level in various categories hosts extrapolating results laboratory experiments cercarial biology natural conditions. study, we evaluated (mussels Mytilus edulis) cercariae two species, Himasthla elongata (Himasthlidae) and Cercaria parvicaudata (Renicolidae), at littoral site White Sea exposing cages uninfected mussels during warm season. This is such study subarctic sea. Mussel was observed May mid-September, but its intensity greatest for approximately month, some moment after 10 July mid-August, when water temperature optimal range emergence both studied species (15‒20°C). During time, accumulated 66.3 ± 6.2% metacercariae H. 79.7 5.3% C. out total number experimental period. We suggest that climate warming latitudes may prolong period temperatures are emergence, thereby intensifying coastal ecosystems.

Язык: Английский

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The effect of host density on parasite infection: a power-law model and an experimental test with trematode larvae infecting mussels

Marine Biology, Год журнала: 2023, Номер 170(2)

Опубликована: Янв. 2, 2023

Язык: Английский

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The Morphological and Molecular Characterization of the Avian Trematodes Harrahium obscurum and Morishitium dollfusi (Digenea: Cyclocoelidae) from the Middle Volga Region (European Russia)

Biology, Год журнала: 2024, Номер 13(8), С. 621 - 621

Опубликована: Авг. 15, 2024

The taxonomic status of many species the family Cyclocoelidae is still unclear. Two cyclocoelids, Harrahium obscurum and Morishitium dollfusi, were collected from air sacs birds (Turdus merula Tringa ochropus) inhabiting Middle Volga region (European Russia). Here, we provide first detailed morphological description these cyclocoelids combine it with molecular phylogenetic analysis in Russia based on partial sequences their 28s rDNA coI mtDNA genes. Specimens both flatworm different host individuals differ slightly body shape size, which probably reflects host-induced intraspecific variability. For time, have shown that a stable character such as length vitelline fields studied digeneans variable at level cannot be used diagnosis.

Язык: Английский

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Morphological vs. molecular identification of trematodes species infecting the edible cockle Cerastoderma edule across Europe

International Journal for Parasitology Parasites and Wildlife, Год журнала: 2024, Номер 25, С. 101019 - 101019

Опубликована: Ноя. 14, 2024

Identifying marine trematode parasites in host tissue can be complicated when there is limited morphological differentiation between species infecting the same species. This poses a challenge for regular surveys of parasite communities socio-economic and ecological importance. Our study focused on identifying digenean bivalve Cerastoderma edule across Europe by comparing molecular identification methods. Cockles were sampled from ten locations to observe under stereomicroscope (morphological identification) isolate individuals phylogenetic analyses using two gene markers, small sub-unit ribosomal (18S) RNA (SSU rDNA) mitochondrial cytochrome c oxidase subunit 1 (cox1). For first time, we compared both each 13 originally identified First, group five which matched results (Bucephalus minimus, Monorchis parvus, Renicola parvicaudatus, Psilostomum brevicolle, Himasthla interrupta). Second, six revealed either misidentifications or cryptic diversity (Gymnophallus choledochus, Diphterostomum brusinae, Curtuteria arguinae, quissetensis, H. elongata, continua). Third, our showed that all sequences expected species, Gymnophallus minutus G. fossarum, two, strongly suggesting only present studied area. clearly demonstrates tools are necessary validate composition. However, with 17 distinct genetic lineages detected, some not fully identified, future studies needed clarify identity status (regular vs. accidental infection) these

Язык: Английский

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No time to relax: Age-dependent infectivity of cercariae in marine coastal ecosystems

Journal of Helminthology, Год журнала: 2023, Номер 97

Опубликована: Янв. 1, 2023

Age dynamics of the ability cercariae two digenean species, Himasthla elongata (Himasthlidae) and Renicola parvicaudatus (Renicolidae), to infect second intermediate host (SIH), mussels (Mytilus edulis), was investigated experimentally. This is first study this kind made on transmitted in intertidal northern seas. The larvae all tested ages (from 0.5 6 hr) were equally successful infecting mussels. finding disagrees with literature data several freshwater digeneans, which are practically incapable SIH during 1-3 hr life. presence a time delay before attainment maximum infectivity (TDMI) may be associated need for physiological maturation very beginning their life environment, broad dispersion, prevention superinfection downstream host. absence TDMI examined our could instability environmental factors marine (wave impact, tidal currents). These promote dispersion biotope prevent potential SIHs. Biological behavioural features also play role. We hypothesize that or does not depend taxonomic affiliation but determined by transmission conditions.

Язык: Английский

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