The parasympathetic and sensory innervation of the proximal and distal colon in male mice DOI Creative Commons
Lixin Wang, Yvette Taché

Frontiers in Neuroanatomy, Год журнала: 2024, Номер 18

Опубликована: Июль 9, 2024

Introduction The distributions of extrinsic neurons innervating the colon show differences in experimental animals from humans, including vagal and spinal parasympathetic innervation to distal colon. neuroanatomical tracing mouse proximal has not been studied details. This study aimed trace locations projecting compared using dual retrograde tracing. Methods sensory were assessed Cholera Toxin subunit B conjugated Alexa-Fluor 488 or 555 injected same mice. Results Retrograde labeled dorsal motor nucleus vagus (DMV) nodose ganglia, while did label lumbosacral cord at L6-S1. Neurons pelvic ganglia which cholinergic projected There more DMV than right ganglion had a higher number left In root (DRG), highest traced L6, those T12. DRG closely intermediolateral column L6 cord. Small percentages with projections both existed DMV, DRG. We also observed long caudal transverse colon, some calbindin immunoreactive, there no retrogradely Discussion These data demonstrated that innervate mice, autonomic intermediate zone do project directly differs humans.

Язык: Английский

Topological segregation of stress sensors along the gut crypt–villus axis DOI
Kouki K Touhara, Nathan D. Rossen, Fei Deng

и другие.

Nature, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Язык: Английский

Процитировано

2
Spinal sensory innervation of the intestine DOI
Rachel L. Wolfson

Current Opinion in Neurobiology, Год журнала: 2025, Номер 90, С. 102973 - 102973

Опубликована: Янв. 31, 2025

Язык: Английский

Процитировано

1
Neurons Co-Expressing GLP-1, CCK, and PYY Receptors Particularly in Right Nodose Ganglion and Innervating Entire GI Tract in Mice DOI Open Access

Elizabeth Lansbury,

Vasiliki Vana, Mari L. Lund

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2053 - 2053

Опубликована: Фев. 26, 2025

Afferent vagal neurons convey gut-brain signals related to the mechanical and chemical sensing of nutrients, with latter also mediated by gut hormones secreted from enteroendocrine cells. Cell bodies these are located in nodose ganglia (NG), right NG playing a key role metabolic regulation. Notably, glucagon-like peptide-1 receptor (GLP1R) primarily innervate muscle layer stomach, distant (GLP-1)-secreting However, co-expression hormone receptors remains unclear. Using RNAscope combined immunohistochemistry, we confirmed GLP1R expression large population neurons, Glp1r, cholecystokinin A (Cckar), Neuropeptide Y Y2 Receptor (Npy2r) being more highly expressed NG, while neurotensin 1 (Ntsr), G protein-coupled (Gpr65), 5-hydroxytryptamine 3A (5ht3a) showed equal expressions left NG. Co-expression analysis demonstrated following: (i) most Cckar, Npy2r co-expressed all three receptors; (ii) nearly Ntsr1- Gpr65-positive both (iii) 5ht3a was subpopulations peptide-hormone-receptor-positive neurons. Retrograde labeling that anterior part stomach preferentially innervated posterior part. The entire gastrointestinal (GI) tract, including distal colon, strongly Most importantly, dual retrograde two distinct tracers identified co-expressing colon. Thus, GLP-1, (CCK), peptide YY (PYY) receptors, predominantly found sample chemical, nutrient-induced along GI tract likely integrate stomach.

Язык: Английский

Процитировано

1
TRPA1-Activated Peptides from Saiga Antelope Horn: Screening, Interaction Mechanism, and Bioactivity DOI Open Access
Chengwei Wang, Chunjie Wu, Linjiang Song

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2119 - 2119

Опубликована: Фев. 27, 2025

Saiga antelope horn (SAH), a rare traditional Chinese medicine, exhibits activities of anti-feverish convulsions and anti-inflammation, whereas its underlying mechanism specific pharmacological components are still unclear. In the present study, transient receptor potential ankyrin 1 (TRPA1), major cation channel was used as target protein to identified TRPA1 high-affinity peptides (THPs) from SAH digests. Firstly, digested under in vitro gastrointestinal conditions. With method affinity ultrafiltration liquid chromatography-mass spectrometry (AUF-LC/MS), about 200 that have interaction with were screened Subsequently, bioactivity databases molecular docking further exploited three THPs, including RCWPDCR, FGFDGDF, WFCEGSF. Furthermore, RIN-14B cells, characterized by high expression on cell surfaces, model investigate biological effect THPs. Immunofluorescence ELISA conducted showed THPs can increase intracellular Ca2+ concentration serotonin (5-HT) secretion cells activating TRPA1, which is evidenced impaired upregulation levels 5-HT after pretreatment inhibitor (HC-030031). Moreover, an analysis Western blots displayed up-regulated synthesis rate-limiting enzyme (TPH1) 5-hydroxytryptophan decarboxylase (Ddc), while reuptake transporter (SERT) down-regulated, suggesting enhance regulating pathway. summary, our findings demonstrate digest via TRPA1-targeted panning, exhibited activation enhanced release cells.

Язык: Английский

Процитировано

1
Limited evidence for anatomical contacts between intestinal GLP-1 cells and vagal neurons in male mice DOI Creative Commons

Newton Cao,

Warda Merchant,

Laurent Gautron

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Окт. 10, 2024

The communication between intestinal Glucagon like peptide 1 (GLP-1)-producing cells and the peripheral nervous system has garnered renewed interest considering availability of anti-obesity anti-diabetic approaches targeting GLP-1 signaling. While it is well-established that can exert influence through paracrine mechanisms, recent evidence suggests possible existence synaptic-like connections neurons, including those vagus nerve. In this study, using a reporter Phox2b-Cre-Tomato mouse model super-resolution confocal microscopy, we demonstrated vagal axons made apparent contacts with less than 0.5% cells. Moreover, immunohistochemistry combined microscopy revealed abundant post-synaptic density 95 (PSD-95) immunoreactivity within enteric plexus lower intestines C57/BL6 mice, virtually none in its mucosa. Lastly, utilizing RNAScope situ hybridization observed expressed generic markers secretory such as Snap25 Nefm, but neither synaptic Syn1 Nrxn2, nor glutamatergic Slc17a7. Through theoretical considerations critical review literature, concluded primarily communicate neurons rather contacts.

Язык: Английский

Процитировано

3
Multi-species probiotic supplement enhances vagal nerve function – results of a randomized controlled trial in patients with depression and healthy controls DOI Creative Commons
Sabrina Mörkl,

Martin Narrath,

Daria Schlotmann

и другие.

Gut Microbes, Год журнала: 2025, Номер 17(1)

Опубликована: Апрель 29, 2025

Major depression (MD) significantly impacts individual well-being and society. The vagus nerve plays a pivotal role in the gut-brain axis, facilitating bidirectional communication between these systems. Recent meta-analyses suggest potential antidepressant effects of probiotics, although their mechanisms remain unclear. This study aimed to assess impact multi-species probiotic (OMNi-BiOTiC® STRESS Repair) on function 43 MD patients healthy controls (HC). Participants received either probiotics or placebo twice daily. Serum stool samples were collected at baseline, 7 days, 28 3 months. Vagus (VN) was evaluated using 24-hour electrocardiography (ECG) for heart rate variability (HRV), alongside microbiome analysis via 16S rRNA sequencing. After months, receiving demonstrated improved morning VN compared HC. participants who group showed significant increase Christensellales, particularly Akkermansia muciniphila along with sleep parameters (use medication, latency) as measured by Pittsburgh Sleep Quality Inventory (PSI). highlights physiological benefits MD, potentially mediated through stimulation. Understanding could lead novel therapeutic approaches management.

Язык: Английский

Процитировано

0
Gut microbiota: a new target for the prevention and treatment of insomnia using Chinese herbal medicines and their active components DOI Creative Commons

Chenxi Wu,

Jinjin Dou,

Xiaoxue Song

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 6, 2025

The emergence of the microbiota-gut-brain axis has opened new avenues for improving sleep quality. Recent studies have revealed a close relationship between insomnia and gut microbiome. Chinese herbal medicines their active components can alter relative abundance sleep-related microbiota by reversing dysbiosis in Improving quality through regulation using medicine its become highly promising therapeutic strategy. This article elucidates how modulates via intricate communication network gut-brain axis. It also reviews latest research on utilizing to regulate enhancing Additionally, it provides insights into potential modulation microbiota.

Язык: Английский

Процитировано

0
Interaction between nutritional factors and the enteric nervous system in inflammatory bowel diseases DOI Creative Commons

Aleksandra Tarasiuk-Zawadzka,

Jakub Fichna

The Journal of Nutritional Biochemistry, Год журнала: 2025, Номер unknown, С. 109959 - 109959

Опубликована: Май 1, 2025

The enteric nervous system (ENS) is a highly conserved, yet complicated network of neurons and glial cells located throughout the gut wall that controls digestive processes gastrointestinal (GI) homeostasis. intestinal epithelium, immune system, microbiota are just few examples cellular networks ENS interacts with on variety levels to maintain GI function. presence or absence nutrients in lumen may cause short- and/or long-term changes neurotransmitter expression, excitability, neuronal survival, which ultimately affect motility, secretion, permeability. Hence, should be identified as key factor initiating coordinated responses nutrients. In this review we summarize current knowledge nutrient-dependent activity how secondary nutrition likelihood developing inflammatory bowel disease. Our findings highlight interact enteroendocrine gut, triggering hormone secretion plays crucial role signaling food-related information brain regulating metabolic such feeding behavior, insulin energy balance; however, complex interactions between nutrients, ENS, require further research understand their contributions disorders potential therapeutic applications treating obesity diseases. Lay Summary: digestion regulate like movement release, affecting metabolism overall health. This highlights need for nutrient-ENS contribute conditions disease, obesity, disorders.

Язык: Английский

Процитировано

0
The parasympathetic and sensory innervation of the proximal and distal colon in male mice DOI Creative Commons
Lixin Wang, Yvette Taché

Frontiers in Neuroanatomy, Год журнала: 2024, Номер 18

Опубликована: Июль 9, 2024

Introduction The distributions of extrinsic neurons innervating the colon show differences in experimental animals from humans, including vagal and spinal parasympathetic innervation to distal colon. neuroanatomical tracing mouse proximal has not been studied details. This study aimed trace locations projecting compared using dual retrograde tracing. Methods sensory were assessed Cholera Toxin subunit B conjugated Alexa-Fluor 488 or 555 injected same mice. Results Retrograde labeled dorsal motor nucleus vagus (DMV) nodose ganglia, while did label lumbosacral cord at L6-S1. Neurons pelvic ganglia which cholinergic projected There more DMV than right ganglion had a higher number left In root (DRG), highest traced L6, those T12. DRG closely intermediolateral column L6 cord. Small percentages with projections both existed DMV, DRG. We also observed long caudal transverse colon, some calbindin immunoreactive, there no retrogradely Discussion These data demonstrated that innervate mice, autonomic intermediate zone do project directly differs humans.

Язык: Английский

Процитировано

2