Microbiota-derived H2S induces c-kit+ cDC1 autophagic cell death and liver inflammation in metabolic dysfunction-associated steatohepatitis

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 5, 2025

Immune dysregulation-induced inflammation serves as a driving force in the progression of metabolic dysfunction-associated steatohepatitis (MASH), while underlying cellular and molecular mechanisms remain largely uncharted. A Western diet (WD) is employed to construct mouse models dysfunction associated steatotic liver disease (MASLD) or MASH. Mass cytometry identifies c-kit+ cDC1 subset whose frequency reduced livers mice patients with MASH compared healthy controls. Adoptive cell transfer protects Moreover, analysis gut microbe sequence shows that WD-fed MASLD/MASH exhibit microbiota dysbiosis, an elevated abundance H2S-producing Desulfovibrio_sp. Transplanting MASH-derived fecal flora, Desulfovibrio_sp., injecting H2S intraperitoneally into MASLD decreases c-kit+cDC1 population exacerbates inflammation. Mechanistically, induces autophagic death c-kit-dependent manner cDC-specific c-kit-/- Atg5-/- mice. We thus uncover microbiota-derived triggers ignites inflammatory cascade

Язык: Английский

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Full-length 16S rRNA Sequencing Reveals Gut Microbiome Signatures Predictive of MASLD in children with obesity

BMC Microbiology, Год журнала: 2025, Номер 25(1)

Опубликована: Март 17, 2025

The gut microbiota plays a crucial role in metabolic dysfunction-associated steatotic liver disease (MASLD). Next-generation sequencing technologies are essential for exploring the microbiome. While recent advancements full-length 16S (FL16S) rRNA offer better taxonomic resolution, whether they establish stronger associations with risk of MASLD remains to be determined. This study utilized long-read FL16S and short-read V3-V4 profile microbiome compositions age-, sex-, BMI-matched case–control pairs obese children without MASLD. A random forest predictive model was employed, using gut-microbiota features selected based on top 35 most abundant taxa or linear discriminant analysis score greater than 3. model's performance evaluated by comparing area under receiver operating characteristic curve (AUC) through tenfold cross-validation method. Subjects exhibited significantly elevated serum alanine aminotransferase, triglycerides, homeostasis assessment insulin resistance levels compared controls. At genus level, detected both were similar, predominantly comprising Phocaeicola Bacteroides, followed Prevotella, Bifidobacterium, Parabacteroides, Blautia. AUC data (86.98%) higher that (70.27%), as determined DeLong's test (p = 0.008). demonstrates children, highlighting its potential real-world clinical applications.

Язык: Английский

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Multi-omics joint analysis: Pachymic acid ameliorated non-alcoholic fatty liver disease by regulating gut microbiota

Food Research International, Год журнала: 2025, Номер unknown, С. 116178 - 116178

Опубликована: Март 1, 2025

Язык: Английский

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Harnessing lactobacillus : a probiotic revolution in combating metabolic dysfunction-associated steatotic liver disease

Critical Reviews in Food Science and Nutrition, Год журнала: 2025, Номер unknown, С. 1 - 18

Опубликована: Май 15, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) involves fat buildup in the and is connected to a disruption gut microbiota reduction beneficial microbiota. Lactobacillus an important probiotic that can grow anaerobic or low-oxygen environment widely used food industry health fields. In recent years, improvement of MASLD by has been extensively studied. However, detailed mechanisms which different species improve have not summarized. this review, we present potential as non-drug approach mitigate MASLD. We will discuss preclinical clinical research backing method ways these probiotics This review mainly presents MASLD, various strains alleviate including improving intestinal permeability inflammation, reducing oxidative stress, restoring metabolites. Future into larger trials help confirm effectiveness using related genera treatment

Язык: Английский

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Gallic acid prevents obesity in mice on a high-fat diet via the gut microbiota-adipose tissue axis

Current Research in Food Science, Год журнала: 2025, Номер 10, С. 101084 - 101084

Опубликована: Янв. 1, 2025

Язык: Английский

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