In
this
study,
a
Ce13d
DNAzyme-based
electrochemical
biosensor
for
the
detection
of
sodium
ions
(Na+)
in
blood
was
developed.
The
gold
nanoparticles
indium
tin
oxide
(ITO)
electrode
modified
with
(Au
NPs),
then
immobilized
double-stranded
DNA
formed
by
substrate
strand
and
enzyme
bound
via
Au-S
link
between
Au
NPs
thiol
Substrate
strand.
presence
Na+,
resulted
DNAzyme
can
preferentially
bind
induced
local
folding
to
form
G-quadruplex-like
structure,
which
enhanced
binding
ability
methylene
blue
(MB).
By
using
specificity
changing
its
conformation,
quantitative
Na+
could
be
achieved.
Thus,
developed
sensing
platform
identify
linear
response
range
10
nM-100
mM
limit
1.03
nM.
To
assess
selectivity
toward
we
tested
some
metal
ions,
including
Li+,
K+,
Cu2+,
Mg2+,
Zn2+
Ca2+.
Furthermore,
proposed
showed
high
satisfactory
results
serum
sample
analysis,
highly
correlated
inductively
coupled
plasma
atomic
emission
spectrometry
method,
confirming
validity
biosensor.
This
work
demonstrated
simple
reliable
method
opens
way
potential
applications
disease
diagnosis
environmental
testing.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 27, 2025
Abstract
Quantitative
detection
of
potassium
ion
(K
+
)
concentration
in
serum
is
great
significance
for
clinical
diagnosis
and
management.
Although
various
methods
are
developed
quantitative
K
concentration,
the
existed
techniques
often
fall
short
realization
rapid
with
small
volume
sample.
Here,
a
novel
smart
hydrogel
strain
sensor
that
combines
poly(
N
‐isopropylacrylamide‐
co
‐benzo‐15‐crown‐5‐acrylamide)
(PNB)
resistance
gauge
efficient
by
converting
amplifying
biochemical
signals
into
easily
measurable
variations.
By
rationally
regulating
PNB
hydrogels
crosslinked
Laponite
XLG
nanosheets,
sensors
can
be
rapidly
achieved
2
min.
Because
sensitive
transformation
enables
measurement
extremely
slight
deformation
hydrogels,
only
one
drop
16
µL
required
to
achieve
blood
sensors.
The
exhibit
exceptional
interference
repeated
performances.
proposed
strategy
not
offers
method
concentrations,
but
also
opens
up
new
window
developing
applications.
Biosensors,
Год журнала:
2025,
Номер
15(2), С. 104 - 104
Опубликована: Фев. 12, 2025
A
microfluidic
system
for
detecting
sodium
ions
(Na+)
has
been
developed,
incorporating
a
micro
finger-pump
chip
and
micro-spectrometer
platform
to
measure
Na+
concentration
in
human
serum.
small
volume
(10
μL)
of
serum
sample
is
introduced
into
the
microchip
reacted
with
preloaded
reagent
mixture
through
two-step
actuation
process.
The
resulting
purple
complex
directed
detection
area
analyzed
using
at
wavelengths
555
666
nm.
then
inversely
derived
from
measured
A555/A666
absorbance
ratio
self-written
software
installed
on
Raspberry
Pi.
entire
process
completed
just
3
min,
offering
significant
advantage
meeting
clinical
needs
compared
traditional
reporting
turnaround
time
several
hours
medical
institutions.
experimental
results
indicate
linear
relationship
between
within
range
1-200
mM,
correlation
coefficient
R2
=
0.9989.
Additionally,
60
samples
collected
chronic
kidney
disease
(CKD)
patients
showed
strong
agreement
those
obtained
conventional
indirect
ion-selective
electrode
(ISE)
method,
achieving
0.9885
an
average
recovery
rate
99.4%.
In
summary,
proposed
provides
practical,
affordable,
rapid
alternative
methods,
making
it
highly
promising
point-of-care
(POC)
testing
applications.
