The Reliable Detection of Homocysteine Using a Biosensor Based on Recombinant Cystathionine β-Synthase and Nanoporous Gold DOI Creative Commons

Zihan Huang,

Yan Gao, Lei Zhang

и другие.

Microorganisms, Год журнала: 2025, Номер 13(3), С. 559 - 559

Опубликована: Март 1, 2025

Given the essential roles of homocysteine (Hcy) and interference cysteine in effectively monitoring human health, this study proposed a synergistic effect strategy that combines unique structural functional properties nanoporous gold (NPG) with selective recognition capability recombinant cystathionine β-synthase (CBS) for sensitive specific detection Hcy. The CBS protein catalytic activity Hcy was successfully produced Escherichia coli BL21 (pET-30a-cbs) using cbs gene from Pseudomonas aeruginosa PAO1. electrochemical mechanism demonstrated electrooxidation H2S, product CBS, an irreversibly surface-controlled process on CBS/NPG/GCE electrode surface. exhibited excellent linearity, high sensitivity reaching 10.43 µA mM−1 cm−2 low limit 1.31 µM. Furthermore, biosensor used to detect urine samples strong anti-interference selectivity (relative standard deviation less than 2.81%), while reducing cysteine. These results confirmed sensor achieved specific, sensitive, reliable rapid homocysteine, making it highly promising practical applications clinical treatment health assessment.

Язык: Английский

The Reliable Detection of Homocysteine Using a Biosensor Based on Recombinant Cystathionine β-Synthase and Nanoporous Gold DOI Creative Commons

Zihan Huang,

Yan Gao, Lei Zhang

и другие.

Microorganisms, Год журнала: 2025, Номер 13(3), С. 559 - 559

Опубликована: Март 1, 2025

Given the essential roles of homocysteine (Hcy) and interference cysteine in effectively monitoring human health, this study proposed a synergistic effect strategy that combines unique structural functional properties nanoporous gold (NPG) with selective recognition capability recombinant cystathionine β-synthase (CBS) for sensitive specific detection Hcy. The CBS protein catalytic activity Hcy was successfully produced Escherichia coli BL21 (pET-30a-cbs) using cbs gene from Pseudomonas aeruginosa PAO1. electrochemical mechanism demonstrated electrooxidation H2S, product CBS, an irreversibly surface-controlled process on CBS/NPG/GCE electrode surface. exhibited excellent linearity, high sensitivity reaching 10.43 µA mM−1 cm−2 low limit 1.31 µM. Furthermore, biosensor used to detect urine samples strong anti-interference selectivity (relative standard deviation less than 2.81%), while reducing cysteine. These results confirmed sensor achieved specific, sensitive, reliable rapid homocysteine, making it highly promising practical applications clinical treatment health assessment.

Язык: Английский

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