Lytic Capsule-Specific Acinetobacter Bacteriophages Encoding Polysaccharide-Degrading Enzymes
Viruses,
Год журнала:
2024,
Номер
16(5), С. 771 - 771
Опубликована: Май 13, 2024
The
genus
Acinetobacter
comprises
both
environmental
and
clinically
relevant
species
associated
with
hospital-acquired
infections.
Among
them,
baumannii
is
a
critical
priority
bacterial
pathogen,
for
which
the
research
development
of
new
strategies
antimicrobial
treatment
are
urgently
needed.
spp.
produce
variety
structurally
diverse
capsular
polysaccharides
(CPSs),
surround
cells
thick
protective
layer.
These
surface
structures
primary
receptors
capsule-specific
bacteriophages,
that
is,
phages
carrying
tailspikes
CPS-depolymerizing/modifying
activities.
Phage
tailspike
proteins
(TSPs)
exhibit
hydrolase,
lyase,
or
esterase
activities
toward
corresponding
CPSs
certain
structure.
In
this
study,
data
on
all
lytic
infecting
genomes
deposited
in
NCBI
GenBank
database
by
January
2024
were
summarized.
149
identified
TSPs
encoded
143
phages,
specificity
(K
specificity)
46
has
been
experimentally
determined
predicted
previously.
63
CPSs,
produced
various
K
types,
was
study
using
bioinformatic
analysis.
A
comprehensive
phylogenetic
analysis
confirmed
prediction
revealed
possibility
genetic
exchange
gene
regions
to
CPS-recognizing/degrading
parts
different
between
morphologically
taxonomically
distant
groups
phages.
Язык: Английский
Characterization of a novel lytic phage vB_AbaM_AB4P2 encoding depolymerase and its application in eliminating biofilms formed by Acinetobacter baumannii
BMC Microbiology,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 8, 2025
Acinetobacter
baumannii
strains
are
a
primary
cause
of
hospital-acquired
infections.
This
bacterium
frequently
causes
biofilm-related
infections,
notably
ventilator-associated
pneumonia
and
catheter-related
which
exhibit
remarkable
resistance
to
antibiotic
treatment,
posing
severe
challenge
in
the
prevention
A.
Therefore,
strategies
eliminate
biofilm
catheters
becoming
increasingly
important.
Phages
capable
lysing
bacteria
have
certain
effect
on
ablation
biofilms.
Sewage
treatment
plant
water
was
collected
for
isolation
phages.
The
morphological,
host
range,
one-step
growth,
temperature
pH
stability,
bactericidal
activity,
sequencing
genomic
analysis
were
performed
characterize
isolated
phage.
three-dimensional
structure
tail
fiber
protein
predicted
by
AlphaFold3.
efficacy
phage
clearing
biofilms
from
24-well
plates
PVC
also
evaluated.
In
this
study,
lytic
vB_AbaM_AB4P2
sewage
water,
showing
clear
plaque
with
halo
zone.
One-step
growth
assays
unveiled
20-minute
latent
period
burst
size
61
forming
unit/cell
(PFU/cell).
At
same
time,
AB4P2
exhibited
stability
at
3–11
temperatures
30–70
°C.
Its
dsDNA
genome
is
composed
45,680
bp
G
+
C
content
46.13%.
Genomic
phylogenetic
situated
as
new
species
Caudoviricetes
class.
possesses
pectin
lyase-like
domain
that
linked
depolymerase
playing
crucial
role
disrupting
Additionally,
it
encodes
lysis
cassette
comprising
endolysin,
holin
Rz-like
spanin,
yet
lacks
any
genes
responsible
virulence
factors.
Phage
can
completely
inhibit
16
h.
plate
polyvinyl
chloride
(PVC)
catheter
model
experiments,
achieved
significant
rate
effectively
killed
live
bacterial
cells
biofilm.
had
good
environmental
strong
ability
destroy
formed
baumannii.
It
exhibits
promising
potential
development
an
alternative
disinfectant
against
hospital.
Not
applicable.
Язык: Английский
In vitro, genomic characterization and pre-clinical evaluation of a new thermostable lytic Obolenskvirus phage formulated as a hydrogel against carbapenem-resistant Acinetobacter baumannii
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Май 17, 2025
The
urgent
threat
of
carbapenem-resistant
Acinetobacter
baumannii
(CRAB)
necessitates
the
development
new
antimicrobial
strategies.
Bacteriophage
(phage)
therapy
is
one
most
promising
alternative
strategies
that
can
be
implemented
to
combat
multidrug-resistant
(MDR)
bacterial
infections.
Herein,
an
A.
phage
VB_AB_Acb75
exhibited
lytic
activity
against
6
CRAB
isolates
(21.43%)
with
stability
at
up
70
°C,
pH
2-12,
and
high
concentrations
organic
solvents
was
isolated
characterized.
transmission
electron
microscope
(TEM)
detected
a
tailed
icosahedral
head
contractile
tail
(myoviral
morphotype).
Oxford
nanopore
sequencing
results
showed
genome
size
45,487
bp,
G
+
C
content
38%,
42
open
reading
frames
(ORFs).
phylogenetic
analysis,
ORF,
TEM
analysis
indicated
belongs
novel
species
in
Obolenskvirus
genus.
Furthermore,
phage-loaded
Carbopol
940
hydrogel
preclinically
evaluated
for
wound
healing
effectiveness
burn-wound
animal
model
infected
isolate.
histology
findings
marked
improvement
through
thick
epidermal
layer
formation
well-organized
fibrous
connective
tissue
covered
by
scab
site
injury,
as
well
ability
eliminate
infection,
compared
control
group.
In
conclusion,
based
on
vitro,
physicochemical
properties,
preclinical
findings,
expected
candidate
clinical
evaluation
CRAB-associated
skin
Язык: Английский
Characterization of an Escherichia coli phage Tequatrovirus YZ2 and its application in bacterial wound infection
Virology,
Год журнала:
2024,
Номер
597, С. 110155 - 110155
Опубликована: Июнь 21, 2024
Язык: Английский
The Biological Characteristics of Mycobacterium Phage Henu3 and the Fitness Cost Associated with Its Resistant Strains
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(17), С. 9301 - 9301
Опубликована: Авг. 27, 2024
Tuberculosis
(TB),
caused
by
Язык: Английский