Journal of Virological Methods, Год журнала: 2023, Номер 325, С. 114870 - 114870
Опубликована: Дек. 10, 2023
Язык: Английский
Molecular Biology Reports, Год журнала: 2025, Номер 52(1)
Опубликована: Фев. 11, 2025
Язык: Английский
Процитировано
2
Current Opinion in Microbiology, Год журнала: 2024, Номер 78, С. 102431 - 102431
Опубликована: Фев. 2, 2024
Horizontal gene transfer occurs via a range of mechanisms, including transformation, conjugation and bacteriophage transduction. Gene agents (GTAs) are an alternative, less-studied route for interbacterial DNA exchange. Encoded within bacterial or archaeal genomes, GTAs assemble into phage-like particles that selflessly package transmit host to recipient bacteria. Several unique features distinguish from canonical phages such as inability self-replicate, thus producing non-infectious particles. also deeply integrated the physiology cell maintained under tight host-regulatory control. Recent advances in understanding structure regulation have provided further insights mechanism is proving increasingly widespread across tree life.
Язык: Английский
Процитировано
8
Viruses, Год журнала: 2025, Номер 17(3), С. 334 - 334
Опубликована: Фев. 27, 2025
Torque Teno Virus (TTV) is a highly prevalent non-pathogenic DNA virus whose plasma levels may be related to the host’s immune status. TTV gained attention about 25 years ago, but its replication not fully understood, nor relationship with host's system. Despite this lack of knowledge, currently being investigated as functional biomarker system in patients immunological damage and inflammatory diseases. Monitoring viral load over time help clinicians making therapeutic decisions regarding immunosuppression well likelihood infectious complications. This review summarizes what we do know enigmatic virus.
Язык: Английский
Процитировано
1
Infection Genetics and Evolution, Год журнала: 2024, Номер 123, С. 105633 - 105633
Опубликована: Июль 3, 2024
Canine parvovirus (CPV) is a significant pathogen in domestic dogs worldwide, causing severe and often fatal disease. CPV comprises three antigenic variants (2a, 2b, 2c) distributed unevenly among several phylogenetic groups. The present study compared genetic variability evolutionary patterns South American populations. We collected samples from puppies suspected of infection the neighboring Argentina Uruguay. Antigenic were preliminarily characterized using PCR-RFLP partial vp2 sequencing. Samples during 2008-2018 mainly 2c variant. In Uruguayan strains (2012-2019), 2a variant wholly replaced 2014. Full-length coding genome sequences with global strains. fell by analysis into two phylogroups (Europe I Asia I). Uruguay clustered Europe group, America, Europe, Asia, Oceania. widely America dog population also being detected wildlife population. formed distinct group Africa, This increasing its distribution worldwide. Our research reveals high adjacent synchronic different CPV. highlight importance ancestral migrations local diversification evolution
Язык: Английский
Процитировано
3
Virus Evolution, Год журнала: 2024, Номер 10(1)
Опубликована: Янв. 1, 2024
Over a decade since its discovery, piscine myocarditis virus (PMCV) remains significant pathogen in Atlantic salmon aquaculture. Despite this impact, the genomic landscape, evolutionary dynamics, and virulence factors of PMCV are poorly understood. This study enhances existing sequence dataset by adding 34 genome sequences 202 new ORF3 from clinical cardiomyopathy syndrome (CMS) cases Norwegian Phylogenetic analyses, also including Faroe Islands Ireland revealed that highly conserved with distinct clustering country origin. Still, single CMS outbreaks display multiple variants, although some was seen case origin, occasional grouping different apparent. Temporal data selected indicated increased diversity population. We hypothesize bottlenecks changing infection dynamics host population, transfer to naïve individuals over time, represent continuous selection pressure on populations. No clear relation found between variants severity heart pathology. However, specific non-synonymous synonymous mutations might impact protein function gene expression efficiency were identified. An additional factor may replication is presence defective viral genomes, novel finding for viruses order
Язык: Английский
Процитировано
3
Sugar Tech, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
Язык: Английский
Процитировано
0
PLoS ONE, Год журнала: 2025, Номер 20(1), С. e0317918 - e0317918
Опубликована: Янв. 30, 2025
Plant viruses pose a significant threat to global agriculture and require efficient tools for their timely detection. We present AutoPVPrimer, an innovative pipeline that integrates artificial intelligence (AI) machine learning accelerate the development of plant virus primers. The uses Biopython automatically retrieve different genomic sequences from NCBI database increase robustness subsequent primer design. design_primers_with_tuning module random forest classifier optimizes parameters provides flexibility experimental conditions. Quality control measures, including evaluation poly-X content melting temperature, reliability. Unique AutoPVPrimer is visualize_primer_dimer module, which supports visual dimers—a feature missing in other tools. Primer specificity validated via BLAST, contributes overall efficiency pipeline. has been successfully applied tomato mosaic virus, proving its adaptability efficiency. modular design allows customization by user extends applicability scenarios. represents advance researchers with effective tool molecular biology experiments. Future developments aim extend compatibility incorporate feedback consolidate as contribution bioinformatics toolbox promising resource advancement virology research.
Язык: Английский
Процитировано
0
PLoS Genetics, Год журнала: 2025, Номер 21(2), С. e1011595 - e1011595
Опубликована: Фев. 5, 2025
Classifying viruses systematically has remained a key challenge of virology due to the absence universal genes and vast genetic diversity viruses. In particular, most dominant diverse group viruses, tailed double-stranded DNA prokaryotes belonging class Caudoviricetes , lack sufficient homology in machinery that unifies them reconstruct inclusive, stable phylogenies these genes. While previous approaches organize phage have managed distinguish various taxonomic levels, methods are limited scalability reproducibility, they do not include modes evolution, like gene gains losses. Here, we present novel, comprehensive, reproducible framework for examining evolutionary relationships phages. this framework, compare genomes based on presences absences fixed set families which is used as binary trait data input into maximum likelihood models heterogeneous rates losses gains. Our resulting phylogeny stably recovers known phages, with without inclusion metagenomic We also quantify mosaicism replication structural among families. results suggest exchanges likely underpin emergence new Additionally, apply large phages (>100 kilobases) map emergences traits associated genome expansion. Taken together, charting organizing improves systemization taxonomy, can unify studies advance our understanding their evolution.
Язык: Английский
Процитировано
0
Archives of Virology, Год журнала: 2025, Номер 170(3)
Опубликована: Фев. 8, 2025
Язык: Английский
Процитировано
0
Journal of Veterinary Research, Год журнала: 2025, Номер 69(1), С. 151 - 157
Опубликована: Март 1, 2025
Abstract Introduction Enzootic nasal adenocarcinoma (ENA) is a cancer that occurs in goats and sheep infected by enzootic tumour virus. Pathologic examinations are useful for distinguishing tumours from inflammatory hyperplasia. The aim of this study was to describe the pathological characteristics ENA. Material Methods Caprine samples were collected examination. tissue sections stained with haematoxylin-eosin periodic acid-Schiff (PAS) processed immunohistochemical staining. Tumour also routine transmission electron microscopy (TEM). Results histopathological structure exhibited both papillary formations superficial regions tubular or acinar deeper layers, representing distinct structural patterns within same adenocarcinoma. cells positive PAS, mitotic figures rare. Low-differentiated nests epithelial–mesenchymal transition phenomena observed. Immunohistochemical analysis showed strongly pancytokeratin cytokeratin (CK)18, moderately CK7, did not express olfactory marker protein. Kiel 67 labelling index approximately 23%. Retrovirus-like particles distributed inside outside TEM. Conclusion origin site ENA epithelium glandular tubules. This low-grade malignant potential. Cytokeratin 7 CK18 can be considered immunophenotypes identifying cells.
Язык: Английский
Процитировано
0