Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(21), С. 11333 - 11333

Опубликована: Окт. 20, 2021

Chronic inflammation is a pathological process where cells of the mesenchymal lineage become major source inflammatory mediators. Platelet-rich fibrin (PRF) has been shown to possess potent anti-inflammatory activity in macrophages, but its impact on not investigated. The aim this study was, therefore, expose cytokines together with lysates generated from liquid platelet-poor plasma (PPP), cell-rich buffy coat layer (BC; concentrated-PRF or C-PRF), and remaining red clot (RC), following centrifugation blood. Heating PPP generates an albumin gel (Alb-gel) that when mixed back C-PRF produces Alb-PRF. Membranes prepared solid PRF were also subjected lysis. We report here PPP, BC, decreased cytokine-induced expression interleukin 6 (IL6) nitric oxide synthase (iNOS) bone marrow-derived ST2 cells. Consistently, greatly phosphorylation nuclear translocation p65 response caused by Pam3CSK4 was reduced accordingly. Moreover, enhanced mediators IL6 iNOS 3T3-L1 pre-adipocyte cells, CCL5 murine calvarial Surprisingly, effective reducing human gingival fibroblasts HSC2 epithelial data present suggest both exert

Язык: Английский

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Titanium platelet–rich fibrin (T-PRF) as high-capacity doxycycline delivery system

Clinical Oral Investigations, Год журнала: 2022, Номер 26(8), С. 5429 - 5438

Опубликована: Май 3, 2022

Язык: Английский

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A Review of In Vivo and Clinical Studies Applying Scaffolds and Cell Sheet Technology for Periodontal Ligament Regeneration

Biomolecules, Год журнала: 2022, Номер 12(3), С. 435 - 435

Опубликована: Март 11, 2022

Different approaches to develop engineered scaffolds for periodontal tissues regeneration have been proposed. In this review, innovations in stem cell technology and engineering focused primarily on Periodontal Ligament (PDL) are discussed analyzed based results from pre-clinical vivo studies clinical trials. Most of those developments include the use polymeric materials with different patterning surface nanotopography printing complex sophisticated multiphasic composite compartments accomodate tissues' architecture. Despite increased effort producing these their undoubtable efficiency guide support tissue regeneration, appropriate source cells is also needed provide new formation various biological mechanochemical cues Extraccellular Matrix (ECM) biophysical stimuli growth differentiation. Cell sheet a novel promising technique that allows obtaining format while preserving ECM components. The right combination factors has not discovered yet efforts still ameliorate regenerative outcomes towards functional organisation developed tissues.

Язык: Английский

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Efficacy of concentrated growth factor versus collagen membrane in reconstructive surgical therapy of peri-implantitis: 3-year results of a randomized clinical trial

Clinical Oral Investigations, Год журнала: 2022, Номер 26(8), С. 5247 - 5260

Опубликована: Май 26, 2022

Abstract Objectives To compare the 3-year clinical and radiographic outcomes of two different reconstructive surgical management peri-implantitis using a bone substitute in combination with either concentrated growth factor (CGF) or collagen membrane (CM). Material methods Fifty-one patients who had at least one implant presenting an intrabony defect were filled xenogenic grafting material covered CGF CM. Clinical assessments carried out baseline postoperative years 1 3. Three composite defined to evaluate treatment success follow-up. The effects possible prognostic indicators on identified by multilevel regression analysis. Results changes probing depth (PD) vertical (VDD) between year 3 presented significantly greater decreases for CM group comparison ( p < 0.05). No significant differences modalities demonstrated regarding outcomes. History periodontitis, VDD baseline, number walls revealed impacts = 0.033; OR 3.50, 0.039; 0.975, 0.024; 7.0 0.019;OR 6.0, respectively). Conclusions seems have slightly better compared membranes therapy peri-implantitis. history VDD, peri-implant configuration could be predictors influencing success. Trial registration ClinicalTrials.gov NCT04769609. relevance For reconstruction defects, may show more favorable

Язык: Английский

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Tensile Strength Essay Comparing Three Different Platelet-Rich Fibrin Membranes (L-PRF, A-PRF, and A-PRF+): A Mechanical and Structural In Vitro Evaluation

Polymers, Год журнала: 2022, Номер 14(7), С. 1392 - 1392

Опубликована: Март 29, 2022

Predictable outcomes intended by the application of PRF (platelet-rich fibrin) derivative membranes have created a lack consideration for their consistency and functional integrity. This study aimed to compare mechanical properties through tensile strength analyze structural organization among produced L-PRF (leukocyte platelet-rich fibrin), A-PRF (advanced A-PRF+ fibrin plus) (original protocols) that varied in centrifugation speed time. (n = 12), 19), 13) were submitted traction test, evaluating maximum average traction. For traction, 0.0020, 0.0022, 0.0010 N·mm−2 obtained A-PRF, A-PRF+, L-PRF, respectively; regarding resistance 0.0012, 0.0015, 0.006 obtained, respectively (A-PRF+ > L-PRF). all groups studied, significant results found. In surface morphology observations SEM, matrix showed highly compact with thick fibers present within interfibrous areas apparent destruction red blood cells leukocytes. The protocol dense composed thin elongated seemed follow preferential orientated direction which platelets well-adhered. Porosity was also evident large diameter spaces whereas most porous platelet concentrate greatest fiber abundance cell preservation. Thus, this concluded higher results, indicating better viscoelastic when stretched two opposing forces.

Язык: Английский

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