A One-Week Elderberry Juice Intervention Augments the Fecal Microbiota and Suggests Improvement in Glucose Tolerance and Fat Oxidation in a Randomized Controlled Trial

Nutrients, Год журнала: 2024, Номер 16(20), С. 3555 - 3555

Опубликована: Окт. 20, 2024

Obesity is a costly and ongoing health complication in the United States globally. Bioactive-rich foods, especially those providing polyphenols, represent an emerging attractive strategy to address this issue. Berry-derived anthocyanins their metabolites are of particular interest for bioactive effects, including weight maintenance protection from metabolic aberrations. Earlier findings small clinical trials suggest modulation substrate oxidation glucose tolerance with mediation prospective benefits attributable gut microbiota, but mixed results appropriate anthocyanin dosing poses challenge. The objective randomized, placebo-controlled study was determine if anthocyanin-dense elderberry juice (EBJ) reproduces glucoregulatory effects observed other berries mediated by microbiota. Overweight or obese adults (BMI > 25 kg/m

Язык: Английский

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Study on the mechanism of action of Jingfang mixture against carrageenan-induced thrombosis in rats based on the gut-liver axis

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

The incidence of thrombosis-related diseases is increasing globally, and it often treated by mechanical thrombectomy, intravenous thrombolysis antiplatelet, but easy to lead drug resistance bleeding complications, which limits the development its treatment. gut-liver axis represents a complex two-way communication system, in intestinal microorganisms not only affect immunity function, also regulate coagulation function liver. Jingfang Mixture (JF) Traditional Chinese Medicine (TCM) that have antithrombotic, mechanism still unclear. This study aimed explore regulatory effect JF on thrombosis rats through carrageenan-induced rat model. results showed after treatment, degree black tail with was significantly reduced, pathological condition improved, four parameters levels factors were adjusted closely normal levels, liver inflammatory reduced. In vitro experiments proved had little platelet aggregation, release, coagulation. our study, can elevate tight junction proteins improve flora disorders, alleviate oxidative stress level liver, short-chain fatty acid feces thrombosis. Of note, relative abundances Clostridia_UCG-014, Ruminococcaceae, Eggerthellaceae, Monoglobaceae Rikenellaceae decreased JFM intervention. Proteomic antithrombotic may be related complement cascade signaling pathway. gene protein expression FGA, FGB, FGG C3 elevated respectively. Finally, we further confirmed therapeutic fecal microbiota transplantation (FMT) experiments, crosstalk contributed improvement summary, this took as breakthrough point coupled 16S rDNA sequencing, non-targeted metabolomics, TMT-based quantitative proteomics comprehensive analysis methods, showing immune system disorder, well reduce damage rats, protective accurately explains multi-target multi-pathway treating diseases, provide reference for clinical application.

Язык: Английский

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Specific dietary fibers steer towards distal colonic saccharolytic fermentation using the microbiota of individuals with overweight/obesity

Food Research International, Год журнала: 2025, Номер unknown, С. 116271 - 116271

Опубликована: Март 1, 2025

Язык: Английский

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Ruminococcus bromii-generated acetate alleviated Clonorchis sinensis-induced liver fibrosis in mice

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 17, 2025

Infection with Clonorchis sinensis (C. sinensis) has the potential to induce liver fibrosis and significantly alter gut microbiota. However, it remains unclear how these changes in microbiota, through gut-liver axis, influence progression of fibrosis. Furthermore, is uncertain whether targeting based on concept could be a therapeutic strategy for alleviating The microbiota alterations C. sinensis-infected mice at multiple time points were analyzed 16S rDNA high-throughput sequencing. Ruminococcus bromii (R.bromii) effect infected was evaluated. Metabolic following produced by R. using short-chain fatty acids (SCFAs) metabolomics. Additionally, conditioned medium (R.b CM) or its metabolites co-cultured two hepatic stellate cell lines (LX2 JS1) vitro assess their anti-fibrotic effects. Finally, RNA sequencing employed investigate specific mechanism which acetate inhibits cells (HSCs) activation. abundance increased during inflammatory stage infection decreased stage. Oral gavage inhibited sinensis-induced while restoring intestinal barrier. activation HSCs upon incubation R.b CM. Acetate identified as key metabolite generated from CM, attenuated vivo. Mechanistically, activating PI3K/AKT signaling pathway prevent sinensis. exerted protective delivering via axis active HSCs. can used probiotic therapy alleviate

Язык: Английский

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WITHDRAWN: Study on the mechanism of action of Jingfang mixture against carrageenan-induced thrombosis in rats based on the gut-liver axis

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish work to be cited a reference. Questions should directed corresponding author.

Язык: Английский

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The link between ultra-processed food consumption, fecal microbiota, and metabolomic profiles in older mediterranean adults at high cardiovascular risk

Nutrition Journal, Год журнала: 2025, Номер 24(1)

Опубликована: Апрель 17, 2025

Ultra-processed food (UPF) consumption has been linked to adverse metabolic outcomes, potentially mediated by alterations in gut microbiota and metabolite production. This study aims explore the cross-sectional longitudinal associations between NOVA-classified UPF consumption, fecal microbiota, metabolome a population of Mediterranean older adults at high cardiovascular risk. A total 385 individuals, aged 55 75 years, were included study. Dietary lifestyle information, anthropometric measurements, stool samples collected baseline after 1-year follow-up. Fecal assessed using 16 S rRNA sequencing liquid chromatography-tandem mass spectrometry, respectively. At baseline, higher was associated with lower abundance Ruminococcaceae incertae sedis (β = - 0.275, P 0.047) concentrations metabolites propionylcarnitine 0.0003, 0.013) pipecolic acid 0.040) feces. Longitudinally, increased reduced Parabacteroides spp. follow-up 0.278, 0.002). High less favorable profiles, suggesting possible link short-chain fatty (SCFA) production, altered mitochondrial energy metabolism, impaired amino metabolism. These findings support reduction promotion dietary patterns rich fiber for better health. Further research is needed confirm these clarify underlying mechanisms. ISRCTN89898870 ( https://doi.org/10.1186/ISRCTN89898870 ).

Язык: Английский

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Dietary preservatives alter the gut microbiota in vitro and in vivo with sex-specific consequences for host metabolic development in a mouse model

American Journal of Clinical Nutrition, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

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Understanding the role of Hedgehog signaling pathway and gut dysbiosis in fueling liver cancer

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Апрель 22, 2025

Язык: Английский

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Microbial Contamination in Urban Marine Sediments: Source Identification Using Microbial Community Analysis and Fecal Indicator Bacteria

Microorganisms, Год журнала: 2025, Номер 13(5), С. 983 - 983

Опубликована: Апрель 25, 2025

We investigated the presence of fecal indicator bacteria Escherichia coli, and other taxa associated with sewage communities in coastal sediments, near beaches reported poor bathing water quality, focusing on influence effluent from a local wastewater treatment plant (WWTP) combined sewer overflows (CSO). Using three-year dataset, we found that treated is significant source sewage-associated viable E. coli sediments no seasonal differences were observed between spring summer samples. CSO events have temporary effect microbial community distinct effluent. Sediments affected by had higher abundances families Lachnospiraceae, Ruminococcaceae, Bacteroidaceae. Sewage releases may also impact natural as marine sulfur-cycling noticed locations where abundant. Microbial contamination at distant known outlets suggests additional sources, such stormwater. This study highlights while can be reservoir contain taxa, their distribution potential origins are complex likely not linked to single source.

Язык: Английский

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Canthaxanthin improves dextran sulfate sodium-induced chronic colitis by modulating intestinal barrier and gut microbiota

Journal of Functional Foods, Год журнала: 2025, Номер 129, С. 106868 - 106868

Опубликована: Май 4, 2025

Язык: Английский

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