Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress DOI Creative Commons

Xiao Y. Wu,

Yaoran Fan,

Keyi Ma

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Дек. 1, 2022

Abstract Temperature is an essential factor affecting the viability of crustaceans, and high temperature can cause damage or even death. The oriental river prawn, Macrobrachium nipponense , important economic aquaculture species in China, Japan, Vietnam. To identify transcriptomic, histological, biochemical response M. reveal their adaptation mechanisms, prawns were placed at 25 ℃, 30 35 ℃ for 24 h. histological damages gills hepatopancreas found under acute heat stress. Additionally, stress enhanced digestive, metabolic, antioxidative capacity by analysis. total RNA isolated sequenced using RNA-Seq method. After filtration, assembly, aggregation, a 131690 unigenes identified. Gene ontology (GO) analysis revealed that differentially expressed genes (DEGs) significantly involved regulation transcription polymerase II, proteolysis, nucleus, cytoplasm, ATP binding. In hepatopancreas, several pathways enriched treatment groups, including neuroactive ligand-receptor interaction, thyroid hormone synthesis, ECM-receptor interaction. And gills, cGMP-PKG signaling pathway, ribosome, calcium enriched. transcriptomic provided insights into thermoregulation molecular mechanisms to

Язык: Английский

Temporal Transcriptomic Profiling Reveals Dynamic Changes in Gene Expression of Giant Freshwater Prawn upon Acute Saline-Alkaline Stresses DOI
Zheyan Chen,

Shouhao Zhu,

Bingbing Feng

и другие.

Marine Biotechnology, Год журнала: 2024, Номер 26(3), С. 511 - 525

Опубликована: Май 15, 2024

Язык: Английский

Процитировано

3
Combined transcriptome and metabolome analysis reveal key regulatory genes and pathways of feed conversion efficiency of oriental river prawn Macrobrachium nipponense DOI Creative Commons

Feiyue Ling,

Yaoran Fan,

Zefei Wang

и другие.

BMC Genomics, Год журнала: 2023, Номер 24(1)

Опубликована: Май 19, 2023

Oriental river prawn Macrobrachium nipponense is an economically important aquaculture species in China, Japan, and Vietnam. In commercial farming, feed cost constitutes about 50 to 65% of the actual variable cost. Improving conversion efficiency culture will not only increase economic benefit, but also save food protect environment. The common indicators used for include ratio (FCR), (FER), residual intake (RFI). Among these, RFI much more suitable than FCR FER during genetic improvement species.In this study, transcriptome metabolome hepatopancreas muscle M. from high low groups, which identified after 75 days, were characterized using combined transcriptomic metabolomic analysis. A total 4540 differentially expressed genes (DEGs) hepatopancreas, 3894 DEGs identified, respectively. mainly enriched KEGG pathways including metabolism xenobiotics by cytochrome P450 (down-regulated), fat digestion absorption (down-regulated) aminoacyl-tRNA biosynthesis (up-regulated), etc. protein glycolysis/gluconeogenesis glutathione At level, was controlled biological such as immune expression reduction nutrients capacity. 445 247 differently metabolites (DEMs) muscle, affected considerably amino acid lipid metabolism.M. higher lower groups have various physiological metabolic capability processes. down-regulated genes, carboxypeptidase A1, 6-phosphofructokinase, long-chain-acyl-CoA dehydrogenase, et. al., nutrients, up-regulated metabolites, aspirin, lysine, response immunity could be potential candidate factors contributed variation nipponense. Overall, these results would provide new insights into molecular mechanism assist selective breeding improve

Язык: Английский

Процитировано

7
Trehalose mediates salinity-stress tolerance in natural populations of a freshwater crustacean DOI Creative Commons
Joana L. Santos, Fabienne Nick, Nikko Adhitama

и другие.

Current Biology, Год журнала: 2024, Номер 34(18), С. 4160 - 4169.e7

Опубликована: Авг. 20, 2024

Salinization poses an increasing problem worldwide, threatening freshwater organisms and raising questions about their ability to adapt. We explored the mechanisms enabling a planktonic crustacean tolerate elevated salinity. By gradually water salinity in clonal cultures from 185 Daphnia magna populations, we showed that salt tolerance strongly correlates with native habitat salinity, indicating local adaptation. A genome-wide association study (GWAS) further revealed major effect of Alpha,alpha-trehalose-phosphate synthase (TPS) gene, suggesting trehalose production facilitates tolerance. Salinity-tolerant animals positive correlation between concentrations, while intolerant failed produce trehalose. Animals non-functional TPS generated through CRISPR-Cas9, supported role stress. Our highlights how keystone animal adapts stress using evolutionary mechanism known bacteria, plants, arthropods.

Язык: Английский

Процитировано

2
Transcriptome, Proteome, Histology, and Biochemistry Analysis of Oriental River Prawn Macrobrachium nipponense under Long-term Salinity Exposure DOI Creative Commons

Yaoran Fan,

Xiao Y. Wu,

Feiyue Ling

и другие.

Frontiers in Marine Science, Год журнала: 2023, Номер 9

Опубликована: Фев. 8, 2023

Salinity is an ecological factor affecting the physiology, survival, and distribution of crustaceans. Additionally, salinity fluctuation detrimentally affects composition biological process As a significant commercial aquaculture species in China, Japan, Southeast Asian countries, oriental river prawn, Macrobrachium nipponense, can tolerate wide range salinity. The transcriptome, proteome, histology, physiology analysis were utilized to explore physiological responses molecular mechanisms tolerance M. nipponense . Through three-month culture, statistic growth trait illustrated relatively excellent performance low salinity, higher exposure significantly affected In terms histological analysis, gills hepatopancreas suffered varying degrees damage. Besides, activities digestive, immune-related, metabolic enzymes calculated. These results indicated that influenced trypsin amylase hepatopancreas, especially 14 ppt. immune-related activated high Notably, activity was 7 ppt, which testified ppt near isotonic point gills, muscle, high-throughput mRNA sequencing revealed 11356, 2227, 1819 differentially expressed genes (DEGs) by comparing 7, 14, 21 groups with 0ppt group, respectively. TMT-labeling proteome identified 439 230 proteins (DEPs) through comparison 0 integration transcriptome several pathways related adaptation enriched, including protein export, cGMP-PKG signaling pathway, Amino sugar nucleotide metabolism, Glycine, serine threonine metabolism. 16 up down-regulated DEGs detected KEGG enrichment ETHE1, BIP, chitinase (E3.2.1.14), SARDH. no regulated recorded correlation hepatopancreas. Thus, optimum survival may from Overall, these provide valuable insights into underlying culture different

Язык: Английский

Процитировано

5
Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress DOI Creative Commons

Xiao Y. Wu,

Yaoran Fan,

Jianbin Feng

и другие.

Aquaculture and Fisheries, Год журнала: 2023, Номер unknown

Опубликована: Май 1, 2023

Temperature is an essential environmental factor affecting the viability of organisms. changes could cause damages or even death. The oriental river prawn, Macrobrachium nipponense, widely distributed in East Asian countries, and has been important economical aquaculture species China since 1990s. This prawn belongs to crustaceans, which a kind ectotherms very sensitive temperature due their relatively low internal metabolic heat production high thermal conductivity. In order reveal adaptation mechanisms, M. nipponense were acclimated at 25 °C for 24 h, successively challenged 30 35 h same tank, respectively. And histological, biochemical transcriptomic analyses carried out on gills hepatopancreas under different temperatures. results showed that degrees histological discovered both °C. digestive, metabolic, antioxidative capacity enhanced A total 2293 3304 differentially expressed genes (DEGs) identified from hepatopancreas, These DEGs significantly involved regulation transcription, proteolysis, nucleus, cytoplasm, metal ion binding, ATP binding by Gene ontology (GO) enrichment analysis. Furthermore, enriched KEGG pathways including neuroactive ligand-receptor interaction, thyroid hormone synthesis, ECM-receptor interaction. cGMP-PKG signaling pathway, ribosome, calcium pathway. be helpful further understanding molecular mechanisms underlying response increasing knowledge freshwater crustaceans context global warming.

Язык: Английский

Процитировано

5
Metabonomic Analysis of Macrobrachium rosenbergii with Iron Prawn Syndrome (IPS) DOI Creative Commons

Xilian Li,

Pei-Jing Shen,

Wenping Jiang

и другие.

Fishes, Год журнала: 2023, Номер 8(4), С. 196 - 196

Опубликована: Апрель 9, 2023

We previously reported on the comparison of proteomic data between seven tissue types a novel “iron prawn” species. However, no transcripts or metabolic information are available for this therefore performed shotgun LC–MS/MS metabonomic and RNA-seq analyses total protein from prawns”. KEGG analysis revealed that largest group consisted 114 pathway proteins, comparing prawns” with normal prawns. A 423 peptides, corresponding to pathways, ABC transporters, starch sucrose metabolism, insulin resistance/secretion, fatty digestion absorption, lipid were identified. The pathways carbohydrate amino acid metabolism decreased in female iron prawns, while organic its derivatives increased. increased male choline cancer glycerophospholipid/histidine/propanoate have been significantly affected Our work provides insight into understanding formation mechanism prawn”.

Язык: Английский

Процитировано

1
The difference in salinity tolerance between cultured and wild sea cucumber Apostichopus japonicus Selenka based on survival, enzyme activity and transcriptome analyses DOI Open Access
Yuming Zhao,

SU Yan-min,

Rongjie Chen

и другие.

Israeli Journal of Aquaculture - Bamidgeh, Год журнала: 2024, Номер 76(4)

Опубликована: Дек. 2, 2024

Salinity is a critical environmental factor affecting the growth, survival, distribution, and physiological processes of sea cucumber, Apostichopus japonicus (Selenka). In this study, we examined survival rates, enzymatic activities, transcriptomes one cultured two wild populations A. Selenka to compare their tolerance across range salinities. Significant differences in rates were observed among three when exposed salinity levels 45, 40, 23, 20, 17 psu. As decreased, activities catalase, Na+-K+-ATPase, amylase superoxide dismutase cucumbers peaked at 23 psu, declined 20 contrast, these enzymes decreased At 40 four significantly but continued increase (P < 0.05). Transcriptomic analysis based on Gene Ontology (GO) function revealed terms “cellular process,” “membrane” “binding” most enriched biological process, cellular component, molecular categories, respectively. Kyoto Encyclopedia Genes Genomes (KEGG) pathway identified “Glycerophospholipid metabolism” “Starch sucrose top 10 pathways. The findings study provide new insights into adaptation cucumbers.

Язык: Английский

Процитировано

0
Transcriptome Reveals Molecular Mechanisms of Neuroendocrine Regulation of Allometric Growth in the Red Swamp Crayfish Procambarus clarkii DOI
Zheyan Chen, Yongqing Wang,

Xianji Tao

и другие.

Marine Biotechnology, Год журнала: 2024, Номер 27(1)

Опубликована: Дек. 2, 2024

Язык: Английский

Процитировано

0
Identification and functional characterization of a novel TRPA1 gene from sea cucumber Apostichopus japonicus and interaction with miR-2013 in response to salt stress DOI Creative Commons
Xin Wei,

Haoran Pan,

Dan Liu

и другие.

Cell Stress and Chaperones, Год журнала: 2023, Номер 28(6), С. 1027 - 1039

Опубликована: Ноя. 1, 2023

Salinity is important abiotic factor influencing sea cucumber aquaculture. This study aimed to identify and functional of a novel transient receptor potential cation channel subfamily A member 1 (TRPA1) involved in salinity stress through interaction with miR-2013 the cucumber. The full-length cDNA sequence was 1369 bp length encoded 138 amino acids. TRPA1 homolog protein hydrophilic without signal peptide predicted spatial structure seven helices eight random coils two major ANK domains. Bioinformatic analysis luciferase reporter assays confirmed as target gene miR-2013. Quantitative PCR revealed that induced upregulation after stress, while showed upregulated expression maximum at 24 h. negatively regulated. Transfection experiments were conducted validate role response. results downregulated transfection mimics, inhibitor. si-TRPA1 resulted downregulation homolog. These findings suggest can regulate under salt highlight importance mimics improved survival rate, inhibitor reduced it. play roles cucumbers adaptation changes.

Язык: Английский

Процитировано

0
Transcriptomic, histological and biochemical analyses of Macrobrachium nipponense response to acute heat stress DOI Creative Commons

Xiao Y. Wu,

Yaoran Fan,

Keyi Ma

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Дек. 1, 2022

Abstract Temperature is an essential factor affecting the viability of crustaceans, and high temperature can cause damage or even death. The oriental river prawn, Macrobrachium nipponense , important economic aquaculture species in China, Japan, Vietnam. To identify transcriptomic, histological, biochemical response M. reveal their adaptation mechanisms, prawns were placed at 25 ℃, 30 35 ℃ for 24 h. histological damages gills hepatopancreas found under acute heat stress. Additionally, stress enhanced digestive, metabolic, antioxidative capacity by analysis. total RNA isolated sequenced using RNA-Seq method. After filtration, assembly, aggregation, a 131690 unigenes identified. Gene ontology (GO) analysis revealed that differentially expressed genes (DEGs) significantly involved regulation transcription polymerase II, proteolysis, nucleus, cytoplasm, ATP binding. In hepatopancreas, several pathways enriched treatment groups, including neuroactive ligand-receptor interaction, thyroid hormone synthesis, ECM-receptor interaction. And gills, cGMP-PKG signaling pathway, ribosome, calcium enriched. transcriptomic provided insights into thermoregulation molecular mechanisms to

Язык: Английский

Процитировано

0