A comprehensive prognostic and immunological analysis of telomere-related lncRNAs in kidney renal clear cell carcinoma DOI Creative Commons
Ji Chen, Dong Zhang,

Xiangbin Ren

и другие.

Aging, Год журнала: 2023, Номер unknown

Опубликована: Окт. 16, 2023

Kidney renal clear cell carcinoma (KIRC) is one of the most prevalent malignant tumors urinary system, with a high recurrence and metastasis rate. Telomeres long non-coding RNAs (lncRNAs) have been documented playing critical roles in cancer progression. However, prognostic significance telomere-related lncRNA (TRLs) KIRC less well-defined. The Cancer Genome Atlas database was applied to retrieve expression profiles corresponding clinical information patients. To create TRLs signature, univariate Cox regression, least absolute shrinkage selection operator analyses were performed. comprised nine TRLs, developed demonstrated superior ability for Additionally, risk score acted as an independent indicator. A nomogram incorporating age, grade, stage, signature-based scores also exhibited excellent predictive accuracy. Several immune activities associated determined by gene function analysis. Further analysis revealed differences status immunity tumor microenvironment between low- high-risk groups. Notably, patients more responsive immunotherapy chemotherapy. summarize, our study new signature consisting that can precisely predict prognosis shown be substantial value mutation burden, thereby contributing framework individualized treatment

Язык: Английский

Cuproplasia and cuproptosis in hepatocellular carcinoma: mechanisms, relationship and potential role in tumor microenvironment and treatment DOI Creative Commons
Ruoyu Zhang, Yunfei Tan, Ke Xu

и другие.

Cancer Cell International, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 9, 2025

Hepatocellular carcinoma (HCC) is the main phenotype of liver cancer with a poor prognosis. Copper vital in function, and HCC cells rely on it for growth metastasis, leading to cuproplasia. Excessive copper can induce cell death, termed cuproptosis. Tumor microenvironment (TME) pivotal HCC, especially immunotherapy, closely related TME pathogenesis. However, how these two mechanisms contribute intriguing. We conducted latest progress literature cuproplasia cuproptosis summarized their specific roles treatment strategies. The relationship role have been deeply summarized. Cuproplasia fosters formation, angiogenesis, whereas may alleviate mitochondrial dysfunction hypoxic conditions TME. Inhibiting enhancing are essential achieving therapeutic efficacy HCC. An in-depth analysis within unveils opposing nature impact regulation. Grasping equilibrium between factors crucial deeper understanding shed light novel directions treating

Язык: Английский

Процитировано

1
A Novel Cuproptosis-Related Signature Identified DLAT as a Prognostic Biomarker for Hepatocellular Carcinoma Patients DOI Open Access
Wendong Bai,

Jun Yu Liu,

Miao Li

и другие.

World Journal of Oncology, Год журнала: 2022, Номер 13(5), С. 299 - 310

Опубликована: Окт. 1, 2022

Background: Hepatocellular carcinoma (HCC) is the most common type of liver cancers, with more than a million cases per year by 2025. Cuproptosis novel form programmed cell death, and caused mitochondrial lipoylation destabilization iron-sulfur proteins triggered copper, which was considered as key player in various biological processes. However, roles cuproptosis-related genes (CRGs) HCC remain largely unknown. Methods: In present study, we constructed validated four CRGs signature for predicting overall survival (OS) patients both The Cancer Genome Atlas (TCGA) International Consortium (ICGC) databases. Results: Patients high risk score showed shorter OS those low score. Functional analysis suggested that CRGs-based prognostic associated metabolism remodeling facilitated cancer progression. addition, reduced infiltration CD8 + T cells increased macrophages were found HCCs from As one CRGs, higher expression dihydrolipoamide S-acetyltransferase (DLAT) accompanied program death ligand 1 (PD-L1) HCC. Further, confirmed DLAT up-regulated correlated poor prognosis clinical cohort. Conclusion: conclusion, our study model identified an independent factor HCC, thus providing new clues understanding association between cuproptosis World J Oncol. 2022;13(5):299-310 doi: https://doi.org/10.14740/wjon1529

Язык: Английский

Процитировано

26
Role of NCF2 as a potential prognostic factor and immune infiltration indicator in hepatocellular carcinoma DOI Creative Commons
Ning Huang, Jing Zhang,

Shuwen Kuang

и другие.

Cancer Medicine, Год журнала: 2023, Номер 12(7), С. 8991 - 9004

Опубликована: Янв. 20, 2023

Abstract Background Hepatocellular carcinoma (HCC) is one of the major causes cancer‐related deaths globally. The tumor microenvironment (TME) plays a crucial role in prognosis and treatment HCC. Hence, it important to exploit new biomarkers for survival surveillance TME estimation Methods HCC samples data was collected from Cancer Genome Atlas (TCGA) International Consortium (ICGC) database, clinical were our center. explored with ESTIMATE (Estimation STromal Immune cells MAlignant Tumor tissues using Expression data), ssGSEA (single sample Gene Sets Enrichment Analysis) CIBERSORT algorithm. Differentially expressed genes analyzed functional enrichment analysis. Immunohistochemistry implemented validate results. Results Based on TCGA we found that Neutrophil Cytosolic Factor 2 (NCF2) significantly associated patients, involved immune‐related biological processes closely some types immunocompetent cells. analysis based NCF2 expression assessed by immunohistochemistry also confirmed NCF2‐positive group had shorter relapse free (RFS) overall (OS) than NCF2‐negative group. Multivariate Cox regression revealed level lymphovascular space invasion (LVSI) independent risk factors patients. Receiver operating characteristic curves showed combination LVSI higher predictive efficacy 1‐year RFS rate 5‐year OS each them alone. Besides, positively M0 M2 macrophages infiltration. Furthermore, correlated CSF1, IL4, IL10, CD206, CD163, CSF1R TGFβ1. Conclusion We proposed predicted an adverse more infiltration

Язык: Английский

Процитировано

15
Cuproptosis regulatory genes greatly contribute to clinical assessments of hepatocellular carcinoma DOI Creative Commons

Changwei Ke,

Shejiao Dai, Fangshi Xu

и другие.

BMC Cancer, Год журнала: 2023, Номер 23(1)

Опубликована: Янв. 7, 2023

Hepatocellular carcinoma (HCC) is a common abdominal cancer with dissatisfactory therapeutic effects. The discovery of cuproptosis lights on new approach for treatment and assessment. So far, there extremely limited research investigating the roles cuproptosis-related (CR) genes in cancers.A novel CR risk signature was constructed using Lasso regression analysis. Its prognostic value assessed via series survival analyses validated three GEO cohorts. effects tumor immune microenvironment (TIM) were explored through CIBERSORT, ESTIMATE, ssGSEA algorithms. Using GESA, we investigated its impacts various metabolism process. somatic mutation features also cBioPortal database. burden, expressions checkpoints, TIDE score, IMvigor 210 cohort, GSE109211 dataset, potential associations score efficacy checkpoint inhibitors (ICIs) sorafenib. Finally, biofunctions DLAT HCC cells ascertained qPCR, immunohistochemistry, colony formation, Transwell assays.FDX1, DLAT, CDKN2A GLS constituted signature. possessed high applicable to validation Meanwhile, it could improve accuracy clinical making-decision benefit traditional model. Moreover, indicative unfavorable anti-tumor response active metabolisms glycolysis nucleotide. As correlation, biomarker predicting ICIs Through qPCR immunohistochemistry detection samples, reconfirmed significantly upregulated samples. Overexpression promote proliferation, migration, invasion HepG2 HuH-7 cells.The greatly contributed assessment HCC. Cuproptosis regulatory gene cancer-promoting capacities expected be promising target

Язык: Английский

Процитировано

12
Prognostic analysis of hepatocellular carcinoma based on cuproptosis -associated lncRNAs DOI Creative Commons

Mingwei Wei,

Libai Lu,

Zongjiang Luo

и другие.

BMC Gastroenterology, Год журнала: 2024, Номер 24(1)

Опубликована: Апрель 23, 2024

Abstract Objectives Cuproptosis represents an innovative type of cell death, distinct from apoptosis, driven by copper dependency, yet the involvement apoptosis-associated long non-coding RNAs (CRLncRNAs) in hepatocellular carcinoma (HCC) remains unclear. This study is dedicated to unveiling role and significance these apoptosis-related lncRNAs within context HCC, focusing on their impact both development disease its prognosis. Methods We conducted analysis gene transcriptomic clinical data for HCC cases sourcing information The Cancer Genome Atlas database. By incorporating cuproptosis-related genes, we established prognostic features associated with lncRNAs. Furthermore, elucidated mechanism prognosis treatment through comprehensive approaches, including Lasso Cox regression analyses, survival analyses samples, as well examinations tumor mutation burden immune function. Results developed a model featuring six lncRNAs: AC026412.3, AC125437.1, AL353572.4, MKLN1-AS, TMCC1-AS1, SLC6A1-AS1. demonstrated exceptional accuracy training validation cohorts patients tumors, showing significantly longer times those categorized low-risk group compared high-risk group. Additionally, our burden, function, Gene Ontology, Kyoto Encyclopedia Genes Genomes pathway enrichment, drug sensitivity, further potential mechanisms which cuproptosis-associated may influence outcome. Conclusions using (lncRNAs) demonstrates promising predictive capabilities immunotherapy outcomes patients. could play crucial patient management optimization immunotherapeutic strategies, offering valuable insights future research.

Язык: Английский

Процитировано

5
Comprehensive Analysis of Cuproptosis-Related Genes in Prognosis and Immune Infiltration of Hepatocellular Carcinoma Based on Bulk and Single-Cell RNA Sequencing Data DOI Open Access
Chenglei Yang,

Yan‐Lin Guo,

Zongze Wu

и другие.

Cancers, Год журнала: 2022, Номер 14(22), С. 5713 - 5713

Опубликована: Ноя. 21, 2022

Background: Studies on prognostic potential and tumor immune microenvironment (TIME) characteristics of cuproptosis-related genes (CRGs) in hepatocellular carcinoma (HCC) are limited. Methods: A multigene signature model was constructed using the least absolute shrinkage selection operator (LASSO) Cox regression analysis. The multivariate cox analysis bulk RNA-seq-based infiltration were performed. results verified two cohorts. enrichment CRGs T cells based single-cell RNA sequencing (scRNA-seq) Real-time polymerase chain reaction (RT-PCR) multiplex immunofluorescence staining performed to verify reliability conclusions. Results: four-gene risk scoring constructed. Kaplan−Meier curve showed that high-risk group had a worse prognosis (p < 0.001). time-dependent receiver operating characteristic (ROC) OS score prediction performance good. These further confirmed validation queue. Meanwhile, Tregs macrophages enriched TIME HCC. Conclusions: CRGs-based could predict Treg significantly HCC, which associated with depletion proliferating cells.

Язык: Английский

Процитировано

17
Regulatory role of lnc-MAP3K13-3:1 on miR-6894-3p and SHROOM2 in modulating cellular dynamics in hepatocellular carcinoma DOI Creative Commons

Kuai Chen,

Manqin Zhu, Qinghua Hu

и другие.

BMC Cancer, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 14, 2025

Hepatocellular carcinoma (HCC) is a prevalent primary liver malignancy and leading cause of cancer-related mortality worldwide. Despite advancements in therapeutic strategies, the 5-year survival rate for individuals undergoing curative resection remains between 10% 15%. Consequently, identifying molecular targets that specifically inhibit proliferation metastasis HCC cells critical improving treatment outcomes. Database analysis using Targetscan identified complementary binding sites human-specific miRNA hsa-miR-6894-3p (hereafter referred to as miR-6894-3p) on SHROOM2, Starbase data suggested potential regulatory interaction lnc-MAP3K13-3:1 miR-6894-3p cancer. This study aimed investigate role regulating miR-6894-3p, with focus its impact proliferation, apoptosis, migration, related cellular processes cancer via SHROOM2 regulation. Quantitative PCR (qPCR) was initially employed measure expression levels three cell lines: HepG2, HuH-7, Li-7. Based these initial assessments, two lines were selected further experimentation. Stable overexpressing developed, transfected mimics or mimic negative control (NC). After 24 h, qPCR utilized quantify relative lnc-MAP3K13-3:1, Caspase9 mRNA each group. Cell analyzed counting Kit-8 assay, while flow cytometry used assess cycle distribution apoptosis. Migration capabilities evaluated through scratch assays, dual-luciferase assays verify interactions SHROOM2. Overexpression transfection resulted increased mRNA, demonstrated by qPCR. The regulated activity lnc-MAP3K13-3:1. Functional showed overexpression inhibited HuH-7 Li-7 cells, promoted reduced migration but enhanced cells. Additionally, significantly proportion G2/M phase S phase. modulated effects migration. Dual-luciferase confirmed direct well These findings indicate regulates targeting thereby influencing other associated HCC.

Язык: Английский

Процитировано

0
Prognostic and immune correlation evaluation of a novel cuproptosis-related genes signature in hepatocellular carcinoma DOI Creative Commons
Zheng Zhou,

Yusong Zhou,

Dongbo Liu

и другие.

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Дек. 14, 2022

Hepatocellular carcinoma (HCC) is one of the world’s malignant tumors with high morbidity and mortality. Cuproptosis a novel form cell death. However, prognostic evaluation immune relevance cuproptosis-related genes (CRGs) in HCC are largely unknown. In our study, we constructed model CRGs performed infiltration, functional analysis, checkpoint drug sensitivity analysis. Systematically elaborated correlation HCC. The results showed that 15 were up-regulated or down-regulated HCC, mutation frequency reached 10.33% CDKN2A having highest frequency. These 19 mainly involved mitochondrion, response metabolic pathways. Five selected (CDKN2A, DLAT, DLST, GLS, PDHA1) constructing enables overall survival patients to be predicted moderate accuracy. Prognostic CRGs, especially CDKN2A, independent factor prognosis, may closely associated immune-cell tumor burden (TMB), microsatellite instability(MSI), checkpoints. CD274, CTLA4, LAG3, PDCD1, PDCD1LG2 SIGLEC15 identified as potential therapeutic targets CD274 correlated highly genes. Quantitative Real-Time PCR (qRT-PCR) immunohistochemical validate mRNA protein expression levels adjacent normal tissues tissues, consistent gene difference conclusion, serve predictors provide insights into cancer therapy.

Язык: Английский

Процитировано

11
From bioinformatics to clinical applications: a novel prognostic model of cuproptosis-related genes based on single-cell RNA sequencing data in hepatocellular carcinoma DOI Creative Commons
Yong Wang, Fenglin Zang, Bing Shao

и другие.

BMC Immunology, Год журнала: 2024, Номер 25(1)

Опубликована: Сен. 9, 2024

Язык: Английский

Процитировано

2
Effect of N6-methyladenosine methylation-related gene signature for predicting the prognosis of hepatocellular carcinoma patients DOI Creative Commons
Xinyu Zheng, Yingyue Zhang, Yun Wang

и другие.

Frontiers in Surgery, Год журнала: 2023, Номер 10

Опубликована: Март 3, 2023

Background and aims Hepatocellular carcinoma (HCC) is a common cause of cancer-related death in humans. Increasing evidence indicates that an imbalance N6-methyladenosine (m6A) methylation linked to the occurrence development cancer. We then developed prognostic model as independent risk factor with which predict prognosis HCC. Methods obtained gene expression clinical data HCC patients from TCGA databases. The value m6A methylation-related genes who had were subjected comprehensive bioinformatics analysis. use RiskScore=∑i=1nCoefi×Xi construct scoring formula. collected pathological specimens 68 HCC, conducted immunohistochemical staining experiments on specimens. Results There was significant correlation between candidate (YTHDF2, METTL14 ZC3H13) overall survival patients. Among patient underwent staining, all cancer tissues positive for METTL14, YTHDF2, ZC3H13 contrast adjacent tissues. Kaplan-Meier results showed low longer time than those high expression. Also, YTHDF2 Finally, lived This result consistent analysis conclusion above. Conclusions Generally, based this study can effectively

Язык: Английский

Процитировано

5