Generating serum-based antibodies from tumor-exposed mice: a new potential strategy for cutaneous squamous cell carcinoma treatment DOI Open Access
Zheng Liu

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 29, 2023

Abstract Current cancer treatment strategies continue to face significant challenges, primarily due tumor relapse, drug resistance, and low efficiency. These issues arise because certain cells adapt the host immune microenvironment evade system. This study presents a new immunotherapy strategy using serum-based antibodies from mice exposed mouse cutaneous squamous cell carcinoma (mCSCC). The experiment was conducted in three stages. In first stage, mCSCC were isolated expanded cultured DMBA/TPA-induced mCSCC. second these injected into healthy stimulate production of anti-tumor antibodies. final therapeutic serum extracted reintroduced tumor-bearing mice. An ELISA assay utilized analyze levels p53, Bcl-xL, NF-κB, Bax. results showed that not only reduced volume but also reversed changes conclusion, this developed immunotherapeutic for treating However, further research is needed fully comprehend mechanism treatment.

Язык: Английский

Generating serum-based antibodies from tumor-exposed mice: a new potential strategy for cutaneous squamous cell carcinoma treatment DOI Creative Commons
Zheng Liu

eLife, Год журнала: 2024, Номер 13

Опубликована: Апрель 16, 2024

Current cancer treatment strategies continue to face significant challenges, primarily due tumor relapse, drug resistance, and low efficiency. These issues arise because certain cells adapt the host immune microenvironment evade system. This study presents a new immunotherapy strategy using serum-based antibodies from mice exposed mouse cutaneous squamous cell carcinoma (mCSCC). The experiment was conducted in three stages. In first stage, mCSCC were isolated expanded cultured DMBA/TPA-induced mCSCC. second these injected into healthy stimulate production of anti-tumor antibodies. final therapeutic serum extracted reintroduced tumor-bearing mice. An ELISA assay utilized analyze levels p53, Bcl-xL, NF-κB, Bax. results showed that not only reduced volume but also reversed changes conclusion, this developed immunotherapeutic for treating However, further research is needed fully comprehend mechanism treatment.

Язык: Английский

Процитировано

0
Generating serum-based antibodies from tumor-exposed mice: a new potential strategy for cutaneous squamous cell carcinoma treatment DOI Open Access
Zheng Liu

Опубликована: Сен. 2, 2024

Current cancer treatment strategies continue to face significant challenges, primarily due tumor relapse, drug resistance, and low efficiency. These issues arise because certain cells adapt the host immune microenvironment evade system. This study presents a new immunotherapy strategy using serum-based antibodies from mice exposed mouse cutaneous squamous cell carcinoma (mCSCC). The experiment was conducted in three stages. In first stage, mCSCC were isolated expanded cultured DMBA/TPA-induced mCSCC. second these injected into healthy stimulate production of anti-tumor antibodies. final therapeutic serum extracted reintroduced tumor-bearing mice. An ELISA assay utilized analyze levels p53, Bcl-xL, NF-κB, Bax. results showed that not only reduced volume but also reversed changes conclusion, this developed immunotherapeutic for treating However, further research is needed fully comprehend mechanism treatment.

Язык: Английский

Процитировано

0
A new potential strategy for cutaneous squamous cell carcinoma treatment by generating serum-based antibodies from tumor-exposed mice DOI Creative Commons
Zheng Liu

eLife, Год журнала: 2024, Номер 13

Опубликована: Окт. 15, 2024

Current cancer treatment strategies continue to face significant challenges, primarily due tumor relapse, drug resistance, and low efficiency. These issues arise because certain cells adapt the host immune microenvironment evade system. This study presents a new immunotherapy strategy using serum-based antibodies from mice exposed mouse cutaneous squamous cell carcinoma (mCSCC). The experiment was conducted in three stages. In first stage, mCSCC were isolated expanded cultured DMBA/TPA-induced mCSCC. second these injected into healthy stimulate production of anti-tumor antibodies. final therapeutic serum extracted reintroduced tumor-bearing mice. An ELISA assay utilized analyze levels p53, Bcl-xL, NF-κB, Bax. results showed that not only reduced volume but also reversed changes conclusion, this developed immunotherapeutic for treating However, further research is needed fully comprehend mechanism treatment.

Язык: Английский

Процитировано

0
Advances in CAR NK Cell Therapy for Targeting and Eradicating Circulating Tumor Cells: Challenges and Solutions for Aging Patients Mini Review DOI Creative Commons
Dmitri Toren, Eugen Ursu, Bogdan Ghilic-Micu

и другие.

Ageing & Longevity, Год журнала: 2024, Номер 4 2024, С. 157 - 173

Опубликована: Ноя. 11, 2024

Chimeric Antigen Receptor (CAR) Natural Killer (NK) cells represent a promising advancement in cancer immunotherapy, particularly for targeting circulating tumor (CTCs) and preventing metastasis. This review examines the latest developments CAR NK cell therapy, including diverse sources, genetic engineering techniques, dual mechanisms of action. Targeting CTCs with shows significant potential aggressive cancers like triple-negative breast (TNBC) pancreatic cancer. The impact aging on function, especially regarding cytotoxicity, cytokine secretion, persistence, poses challenges elderly patients, but strategies such as interleukin-15 metabolic interventions offer solutions. also addresses current limitations, poor persistence immunosuppressive microenvironments low solid infiltration, while proposing combination therapies to enhance effectiveness. Although still earlier clinical stages compared T cells, cells’ safety profile MHC-independent recognition make them versatile therapeutic option. Future directions include optimizing improving developing age-adapted patients. _________________________________________________________________________________________ Keywords: Circulating (CTCs), Cell Therapy, Aging

Язык: Английский

Процитировано

0
Generating serum-based antibodies from tumor-exposed mice: a new potential strategy for cutaneous squamous cell carcinoma treatment DOI Open Access
Zheng Liu

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 29, 2023

Abstract Current cancer treatment strategies continue to face significant challenges, primarily due tumor relapse, drug resistance, and low efficiency. These issues arise because certain cells adapt the host immune microenvironment evade system. This study presents a new immunotherapy strategy using serum-based antibodies from mice exposed mouse cutaneous squamous cell carcinoma (mCSCC). The experiment was conducted in three stages. In first stage, mCSCC were isolated expanded cultured DMBA/TPA-induced mCSCC. second these injected into healthy stimulate production of anti-tumor antibodies. final therapeutic serum extracted reintroduced tumor-bearing mice. An ELISA assay utilized analyze levels p53, Bcl-xL, NF-κB, Bax. results showed that not only reduced volume but also reversed changes conclusion, this developed immunotherapeutic for treating However, further research is needed fully comprehend mechanism treatment.

Язык: Английский

Процитировано

0