Novel Approaches to Reducing Gastric Cancer Risk through Electromagnetic Signal Treatment of Pork Meat and Its Effects on MAPK Pathway Modulation DOI
Elsa Easter Justine, Abdus Samad,

Sanjeevram Dhandapani

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Abstract The consumption of red meat, such as pork, beef, and lamb, has been associated with an incidence gastric cancer (GC) due to certain processing preparation methods. However, electromagnetic signal (EMS) exposure demonstrated beneficial effects on food preparation, potentially improving quality reducing risk factors GC. In our study, pork meat was treated EMS, this investigated for its potential reduce GC via in vitro transcriptomic approaches. 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide quantitative reverse transcription polymerase chain reaction assays were used explore the cytotoxic apoptotic EMS-treated (EMS-T) untreated (EMS-UT) extract cells (KATO-III S1M). results indicated that KATO-III S1M exhibited highest inhibitory effect, 32.8% 24.53% inhibition, respectively, following treatment 500 μg/mLEMS-T compared EMS-UT treatment. Additionally, markers, CASP3 CASP9, BAX/BCL2ratio suggested apoptosis initiated upon EMS-T extract. Transcriptomic analysis further revealed led 217 uniquely differentially expressed genes in cells, 135 suppressed 82 stimulated genes. Notably, mitogen-activated protein kinase (MAPK) pathway emerged a notably interconnected signaling pathway, key genes, DUSP5, MAP4K2, TGFB3, CACNA2D2, CD14, CACNA1H, EREG, showing significant interactions. Specifically, resulted suppression MAP4K2, TGFB3, CD14, while DUSP5and CACNA2D2 were contrasting gene expression profiles observed patients healthy individuals according Cancer Genome Atlas data. Overall, these findings suggest may offer novel approach by modulating MAPK

Язык: Английский

Targeted protein degradation with small molecules for cancer immunotherapy DOI Creative Commons
Zichao Yang,

Jianwei Xu,

Xixiang Yang

и другие.

Asian Journal of Pharmaceutical Sciences, Год журнала: 2025, Номер unknown, С. 101058 - 101058

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
NK Cell‐Based Cancer Immunotherapies: Current Progress, Challenges and Emerging Opportunities DOI
Mohammad Y. Alshahrani, Subasini Uthirapathy, Abhinav Kumar

и другие.

Journal of Biochemical and Molecular Toxicology, Год журнала: 2024, Номер 38(11)

Опубликована: Ноя. 1, 2024

Natural killer (NK) cells are vital innate immune that play a crucial role in cancer therapy. They targeted by therapies designed to modulate, retain, and enhance their antitumor function vivo. In addition whole-cell therapy, NK cell-derived exosomes (NDEs) offer high safety easily subject chemical, biological, immunological modifications. This makes them suitable for use combination with various current efficacy, treatment outcomes, reduce side effects review aims outline summarizes the potential of cells, as well challenges opportunities cell-based immunotherapies such allogeneic autologous cell transplantation, CAR cytokines monoclonal antibodies. The also explores dual regulatory tumor on highlights NDEs, either alone or other therapies, reprogram immunosuppressive microenvironment expedite therapeutic immunotherapy future clinical research.

Язык: Английский

Процитировано

2
Integrated analysis of single-cell, spatial and bulk RNA-sequencing identifies a cell-death signature for predicting the outcomes of head and neck cancer DOI Creative Commons

Yue Pan,

Fei Lei, Shihua Wang

и другие.

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 7, 2024

Background Cell death plays an essential role in carcinogenesis, but its function the recurrence and postoperative prognosis of head neck cancer (HNC), which ranks as 7 th most common malignancy globally, remains unclear. Methods Data from five main subtypes HNC related single-cell RNA sequencing (scRNA-seq) were recruited to establish a atlas, distribution cell models (CDMs) across different tissues well analyzed. Bulk RNA-seq Cancer Genome Atlas Program (TCGA) dataset was subjected machine learning-based integrative procedure for constructing consensus death-related signature risk score (CDRscore) model validated by external data. The biofunctions including expression analysis, immune infiltration, genomic mutations, enrichment analysis cellchat compared between high- low- groups categorized this CDRscore model. Finally, samples laryngeal squamous (LSCC) conducted spatial transcriptomics (ST) further validate results Results T cells patients manifested highest levels while HPV infection attenuates malignant based on atlas. CDMs are positively correlated with tumor-cell stemness, immune-related infiltrated. A established transcription ten prognostic genes ( MRPL10 , DDX19A NDFIP1 PCMT1 HPRT1 SLC2A3 EFNB2 HK1 BTG3 MAP2K7 ). It functions independent factor overall survival displays stable powerful performance GSE41613 GSE65858 datasets. Patients high worse survival, more active epithelial mesenchymal transition, TGF-β-related pathways hypoxia, higher exhausted markers, stronger TP53 mutation. ST LSCC showed that spots high-risk scores colocalized TGF-β proliferating cells, additionally, have negative correlation TCR signaling positive association LAG3 transcription. Conclusion could be utilized indicator HNC.

Язык: Английский

Процитировано

1
Novel Approaches to Reducing Gastric Cancer Risk through Electromagnetic Signal Treatment of Pork Meat and Its Effects on MAPK Pathway Modulation DOI
Elsa Easter Justine, Abdus Samad,

Sanjeevram Dhandapani

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

Abstract The consumption of red meat, such as pork, beef, and lamb, has been associated with an incidence gastric cancer (GC) due to certain processing preparation methods. However, electromagnetic signal (EMS) exposure demonstrated beneficial effects on food preparation, potentially improving quality reducing risk factors GC. In our study, pork meat was treated EMS, this investigated for its potential reduce GC via in vitro transcriptomic approaches. 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide quantitative reverse transcription polymerase chain reaction assays were used explore the cytotoxic apoptotic EMS-treated (EMS-T) untreated (EMS-UT) extract cells (KATO-III S1M). results indicated that KATO-III S1M exhibited highest inhibitory effect, 32.8% 24.53% inhibition, respectively, following treatment 500 μg/mLEMS-T compared EMS-UT treatment. Additionally, markers, CASP3 CASP9, BAX/BCL2ratio suggested apoptosis initiated upon EMS-T extract. Transcriptomic analysis further revealed led 217 uniquely differentially expressed genes in cells, 135 suppressed 82 stimulated genes. Notably, mitogen-activated protein kinase (MAPK) pathway emerged a notably interconnected signaling pathway, key genes, DUSP5, MAP4K2, TGFB3, CACNA2D2, CD14, CACNA1H, EREG, showing significant interactions. Specifically, resulted suppression MAP4K2, TGFB3, CD14, while DUSP5and CACNA2D2 were contrasting gene expression profiles observed patients healthy individuals according Cancer Genome Atlas data. Overall, these findings suggest may offer novel approach by modulating MAPK

Язык: Английский

Процитировано

0