Linc00963 up-regulation alleviates postmenopausal osteoporosis through suppression of miR-506-3p DOI Creative Commons
Qiang Li, Jian Zhao, Xiaoxia Yang

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 11, 2025

This study aimed to investigate the regulatory effect of linc00963 on postmenopausal osteoporosis and potential molecular mechanisms. Taking MC3T3-E1 cells as object, a cell cycle assay was used evaluate proliferation. mRNA levels Runx2, OCN, collagenia-1, OPG, RANKL RANK were detected. Dual luciferase reporter verified targeting relationship between miR-506-3p. A rat model established after ovariectomy in 32 Sprague-Dawley rats. The rats divided into sham group, OVX overexpression blank plasmid group. bone mineral density (BMD) femur measured by X-ray densitometer. Serum expression detected RT-qPCR. protein ALP, BGP serum ELISA. Cell studies have shown that alleviates down-regulating Animal showed compared with level, BMD, Ca, P, LEP, SOD, OPG group significantly decreased, while body weight, BGP, IL-6, IL-13, RANKL, increased. Compared use can improve above indexes play corresponding therapeutic menopausal Linc00963 is involved pathogenesis up-regulating miR-506-3p activating OPG/RANKL/RANK pathway. expected be target for osteoporosis.

Язык: Английский

Linc00963 up-regulation alleviates postmenopausal osteoporosis through suppression of miR-506-3p DOI Creative Commons
Qiang Li, Jian Zhao, Xiaoxia Yang

и другие.

Journal of Orthopaedic Surgery and Research, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 11, 2025

This study aimed to investigate the regulatory effect of linc00963 on postmenopausal osteoporosis and potential molecular mechanisms. Taking MC3T3-E1 cells as object, a cell cycle assay was used evaluate proliferation. mRNA levels Runx2, OCN, collagenia-1, OPG, RANKL RANK were detected. Dual luciferase reporter verified targeting relationship between miR-506-3p. A rat model established after ovariectomy in 32 Sprague-Dawley rats. The rats divided into sham group, OVX overexpression blank plasmid group. bone mineral density (BMD) femur measured by X-ray densitometer. Serum expression detected RT-qPCR. protein ALP, BGP serum ELISA. Cell studies have shown that alleviates down-regulating Animal showed compared with level, BMD, Ca, P, LEP, SOD, OPG group significantly decreased, while body weight, BGP, IL-6, IL-13, RANKL, increased. Compared use can improve above indexes play corresponding therapeutic menopausal Linc00963 is involved pathogenesis up-regulating miR-506-3p activating OPG/RANKL/RANK pathway. expected be target for osteoporosis.

Язык: Английский

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