Higher percentage of CD34+ stem cells and elevated efficacy in androgenetic alopecia treatment observed in CGF prepared from 640 nm laser‐pretreated blood: A preliminary study

Journal of Cosmetic Dermatology, Год журнала: 2024, Номер 23(6), С. 2249 - 2255

Опубликована: Март 1, 2024

Abstract Background Concentrated growth factor (CGF) injection has proven effective in treating androgenetic alopecia (AGA). The primary mechanism of CGF AGA is thought to be the CD34+ stem cells and platelets‐associated factors being injected into scalp. efficacy may rely on activation level these platelets. 640 nm laser a United States Food Drug Administration approved treatment that activates follicle cells. Therefore, we hypothesize pretreating with further activate platelets, thereby improving AGA. Objective This study aims investigate whether pretreated (640CGF) greater effect than non‐pretreated (N640CGF) evaluate pretreatment changed cell percentage. Methods enrolled 10 patients (8 male, 2 female) aged 18–60 years who received injections. 640CGF group was at an energy density 4 J/cm , 30 cm irradiation distance for min. Half scalp treated 640CGF, whereas other half N640CGF. prepared by doctor did not know which blood tube had been pretreated. evaluated using trichoscope 1 month after injection. Results All (100%) participated follow‐up visit, higher quantity new hairs observed side N640CGF ( p = 0.019). Additionally, fewer malnourished were 0.015). No serious adverse events reported. Conclusions A percentage improved could from laser‐pretreated blood.

Язык: Английский

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Effectiveness of the Association of Fibrin Scaffolds, Nanohydroxyapatite, and Photobiomodulation with Simultaneous Low-Level Red and Infrared Lasers in Bone Repair

Materials, Год журнала: 2024, Номер 17(17), С. 4351 - 4351

Опубликована: Сен. 3, 2024

Biomaterials and biopharmaceuticals for correcting large bone defects are a potential area of translational science. A new bioproduct, purified from snake venom fibrinogen buffalo blood, aroused interest in the repair venous ulcers. Expanding uses, it has also been used to form biocomplexes combination with grafts, associated physical therapies or alone. The aim this preclinical study was evaluate low-level laser photobiomodulation (PBM) critical calvaria rats filled nanohydroxyapatite (NH) heterologous fibrin biopolymer (HFB). Sixty animals were used, divided into six groups (n = 10 each): G1 (NH); G2 (HFB); G3 (NH + HFB); G4 PBM); G5 (HFB G6 HFB PBM). PBM simultaneously red (R) infrared (IR) light emission, applied intraoperatively twice week, until end experiment at 42 days. Microtomography, formation can be seen initially margins defect, more evident G5. Microscopically, demonstrated immature disorganized trabeculation 14 days, remnants grafting materials. At percentage formed higher all groups, especially (HFB, 45.4 ± 3.82), collagen fibers degree maturation yellowish-green color birefringence analysis Picrosirius-red. Therefore, is concluded that showed greater effectiveness process presents future clinical studies.

Язык: Английский

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The effect of low-level laser therapy on osteoclast differentiation: Clinical implications for tooth movement and bone density

Journal of Dental Sciences, Год журнала: 2024, Номер 19(3), С. 1452 - 1460

Опубликована: Март 30, 2024

Язык: Английский

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A Preliminary Investigation into the Effect of Low-Energy Lasers on Dental Pulp Stem Cell Proliferation and the Associated Mechanism

Frontiers in Bioscience-Landmark, Год журнала: 2024, Номер 29(6), С. 211 - 211

Опубликована: Июнь 11, 2024

Background: Dental pulp stem cells (DPSCs) have self-renewal and multidirectional differentiation potentials. As such, DPSCs a wide range of clinical applications. Low-level laser therapy (LLLT) has positive photobiostimulatory effects on cell proliferation, angiogenesis, osteogenic differentiation, bone regeneration, fracture healing. However, there been few studies the effect low-energy lasers DPSC proliferation. Methods: were obtained from dental tissue. The LLLT proliferation associated mechanisms investigated by in vitro culture irradiation. Results: with energy densities 3.5 J/cm2 14 J/cm2promoted DPSCs. Differential protein expression suggested stimulation involved PI3K-Akt Rap1 signaling pathways, as well apoptosis-related pathway. Conclusion: This preliminary study demonstrated that pro-proliferative DPSCs, identified possible mechanisms. Our findings provide theoretical basis for application suggest novel strategies treatment related diseases.

Язык: Английский

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Photobiomodulation Literature Watch October 2023

Photobiomodulation Photomedicine and Laser Surgery, Год журнала: 2024, Номер 42(8), С. 568 - 571

Опубликована: Авг. 1, 2024

Язык: Английский

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