Environmental Entomology,
Год журнала:
2020,
Номер
49(3), С. 593 - 600
Опубликована: Март 28, 2020
In
2014-2019,
we
conducted
six
experiments
in
north-central
Georgia
an
attempt
to
verify
the
aggregation
pheromone
response
of
ambrosia
beetle
Gnathotrichus
materiarius
(Fitch)
(Coleoptera:
Curculionidae:
Scolytinae:
Scolytini:
Corthylina)
sulcatol
known
be
produced
by
male
G.
materiarius;
failed
catch
any
materiarius.
However,
did
find
that
another
corthyline
species
Monarthrum
mali
was
attracted
(R)-(-)-sulcatol,
whereas
longhorn
Leptostylus
asperatus
(Haldeman)
Cerambycidae:
Lamiinae)
(S)-(+)-sulcatol.
Attraction
both
unaffected
respective
antipodes.
Ethanol
enhanced
attraction
traps
baited
with
sulcatol.
at
least
one
experiment,
ethanol-baited
for
Xylosandrus
crassiusculus
(Motschulsky),
Xyleborus
spp.,
and
Hypothenemus
spp.
but
reduced
Cnestus
mutilatus
(Blandford)
Scolytinae).
Additionally,
ethanol
racemic
[50%
(S)-(+):
50%
(R)-(-)]
caught
greatest
numbers
four
predators:
Coptodera
aerata
Dejean
Carabidae),
Colydium
lineola
Say
Zopheridae),
Madoniella
dislocata
(Say),
Pyticeroides
laticornis
(Say)
Cleridae).
not
Temnoscheila
virescens
(F.)
Trogossitidae).
Information
on
interspecific
relationships
within
forested
communities
may
help
us
better
determine
roles
these
maintaining
stable
resilient
ecosystems.
Full
taxonomic
characterisation
of
fungal
communities
is
necessary
for
establishing
ecological
associations
and
early
detection
pathogens
invasive
species.
Complex
fungi
are
regularly
characterised
by
metabarcoding
using
the
Internal
Transcribed
Spacer
(ITS)
Large-Subunit
(LSU)
gene
rRNA
locus,
but
reliance
on
a
single
short
sequence
fragment
limits
confidence
identification.
Here
we
link
from
ITS2
LSU
D1-D2
regions
to
characterise
associated
with
bark
beetles
(Scolytinae),
likely
vectors
several
tree
pathogens.
Both
markers
revealed
similar
patterns
overall
species
richness
response
key
variables
(beetle
species,
forest
type),
identification
against
respective
reference
databases
various
classifiers
poor
resolution
towards
lower
levels,
especially
level.
Thus,
Operational
Taxonomic
Units
(OTUs)
could
not
be
linked
via
across
ITS
fragments.
However,
phylogenetic
trees
(focused
epidemiologically
important
Sordariomycetes)
placed
OTUs
obtained
either
marker
relative
sequences
entire
cistron
that
includes
both
loci
demonstrated
largely
distribution
LSU-derived
OTUs.
Sensitivity
analysis
congruence
in
suggested
biologically
most
defensible
threshold
values
OTU
delimitation
Sordariomycetes
98%
99%
D1-D2.
Studies
canonical
barcode
require
corroboration
additional
loci.
Phylogenetic
aligned
full
shows
higher
success
rate
greater
accuracy
compared
probabilistic
classifiers.
Management of Biological Invasions,
Год журнала:
2020,
Номер
11(4), С. 677 - 688
Опубликована: Янв. 1, 2020
A
multi-lure
approach
for
trapping
a
wide
variety
of
Cerambycidae
was
tested
at
the
Port
Vienna
in
2017
and
2018,
high
risk
area
introduction
invasive
tree
pests
transported
wood
packaging
material.Traps
were
deployed
port
an
adjacent
broadleaved
forest.A
second
experimental
site
set
up
pine
forest
eastern
Austria
not
influenced
by
imports.Blends
cerambycid
pheromone
compounds
tested:
Blend
1
mixing
four
components
various
Lamiinae
Spondylidinae,
2
Cerambycinae
Prioninae,
3
combining
eight
volatiles.Additionally,
host
kairomones
ethanol
(-)-α-pinene
added
as
general
attractants.A
total
30
species
detected
with
traps
2017;
36
2018.The
full
blend
(Blend
plus
(-)-α-pinene)
caught
significantly
more
than
specific
Blends
2.
Addition
to
increased
number
trapped
individuals
but
species.Overall,
we
28
(11
species)
(23
23
forest.No
alien
two
years.Trapping
multiple
lures
could
be
employed
surveillance
programs
against
potentially
cerambycids
areas
introduction.
Abstract
Molecular
assays
based
on
qPCR
TaqMan
Probes
were
developed
to
identify
three
species
of
the
genus
Xylosandrus
,
X.
compactus,
crassiusculus
and
germanus
(Coleoptera
Curculionidae
Scolytinae).
These
ambrosia
beetles
are
xylophagous
alien
Europe,
causing
damages
many
ornamental
fruiting
trees
as
well
shrubs.
DNA
extraction
was
carried
out
from
adults,
larvae
biological
samples
derived
insect
infested
plants.
For
compactus
segments
galleries
in
thin
twigs
cut
processed;
case
raw
frass
extruded
exit
holes
used,
while
extracted
small
wood
chips
removed
around
holes.
The
inclusive
for
target
exclusive
all
non-target
tested.
LoD
3.2
pg/µL
0.016
ng/µL
woody
matrices
other
two
species.
Both
repeatability
reproducibility
estimated
adults
samples,
showing
very
low
values
ranging
between
0.00
4.11.
Thus,
proposed
diagnostic
resulted
be
efficient
also
used
extraction,
demonstrating
applicability
protocol
absence
dead
specimens
or
living
stages.
Alpine Entomology,
Год журнала:
2023,
Номер
7, С. 45 - 55
Опубликована: Май 19, 2023
Identifying
alien
species
is
important
to
ensure
the
early
detection
of
biological
invasions
and
survey
shifts
in
distributions
context
global
change.
Here,
we
report
on
three
bark
ambrosia
beetles
newly
detected
Switzerland:
Cyclorhipidion
distinguendum
(Eggers,
1930),
C.
pelliculosum
(Eichhoff,
1878),
Hypothenemus
eruditus
(Westwood,
1834).
These
were
recorded
for
first
time
during
a
comprehensive
saproxylic
accross
major
forest
types
along
an
altitudinal
gradient
entire
growing
season
southern
Alps,
canton
Ticino.
Their
local
abundance
number
occurrences
different
lowland
habitats,
including
alluvial
forests
national
importance,
indicates
that
all
are
already
naturalized.
Given
their
polyphagy,
it
likely
will
become
more
extensively
distributed
across
Switzerland,
with
yet
unknown
environmental
impact.
Insects,
Год журнала:
2021,
Номер
12(10), С. 949 - 949
Опубликована: Окт. 18, 2021
Many
bark
beetles
of
the
subfamily
Scolytinae
are
most
economically
important
insect
pests
coniferous
forests
worldwide.
In
this
study,
we
sequenced
mitochondrial
genomes
eight
beetle
species,
including
Dendroctonus
micans,
Orthotomicus
erosus,
Polygraphus
poligraphus,
Dryocoetes
hectographus,
Ips
nitidus,
typographus,
subelongatus,
and
hauseri,
to
examine
their
structural
characteristics
determine
phylogenetic
relationships.
We
also
used
previously
published
genome
sequence
data
from
other
species
identify
localize
studied
within
phylogeny.
Their
gene
arrangement
matched
presumed
ancestral
pattern
these
beetles.
Start
stop
codon
usage,
amino
acid
abundance,
relative
usage
frequencies
were
conserved
among
Genetic
distances
between
ranged
0.037
0.418,
evolutionary
rates
protein-coding
genes
0.07
for
COI
0.69
ND2.
Our
results
shed
light
on
relationships
taxonomic
status
several
in
highlight
need
further
sequencing
analyses
revisions
additional
species.
Journal of Integrated Pest Management,
Год журнала:
2022,
Номер
13(1)
Опубликована: Янв. 1, 2022
Abstract
We
provide
scientists
and
decision-makers
with
up-to-date
information
on
Megaplatypus
mutatus
(Coleoptera:
Curculionidae:
Platypodinae),
a
forest
insect
native
to
South
America
that
has
invaded
other
regions.
Emphasis
is
given
may
prove
relevant
for
the
development
of
early
detection,
containment
management
programs
improved
risk
analyses.
The
increase
in
global
movement
people
goods,
coupled
expected
climatic
scenarios,
suggests
M.
arrive
establish
populations
new
areas.
major
impact
this
pest
always
been
reported
exotic
tree
species,
even
within
its
range.
absence
coevolutionary
history
‘naïve’
host
trees
factor
when
analyzing
understanding
magnitude
problem
posed
by
beetle
fungi
associated
it.
Notably,
among
preferred
hosts
are
widely
planted
Eucalyptus
Populus
spp.,
facilitating
invasion
into
regions
posing
threat
commercial
forestry.