RNA Interference in Insects: From a Natural Mechanism of Gene Expression Regulation to a Biotechnological Crop Protection Promise

Biology, Год журнала: 2024, Номер 13(3), С. 137 - 137

Опубликована: Фев. 21, 2024

Insect pests rank among the major limiting factors in agricultural production worldwide. In addition to direct effect on crops, some phytophagous insects are efficient vectors for plant disease transmission. Large amounts of conventional insecticides required secure food worldwide, with a high impact economy and environment, particularly when beneficial also affected by chemicals that frequently lack desired specificity. RNA interference (RNAi) is natural mechanism gene expression regulation protection against exogenous endogenous genetic elements present most eukaryotes, including insects. Molecules double-stranded (dsRNA) or highly structured substrates cellular enzymes produce several types small RNAs (sRNAs), which play crucial role targeting sequences transcriptional post-transcriptional silencing. The relatively simple rules underlie RNAi regulation, mainly based Watson–Crick complementarity, have facilitated biotechnological applications these mechanisms. This includes promise using engineered dsRNA molecules, either endogenously produced crop plants exogenously synthesized applied onto as new generation specific, sustainable, environmentally friendly insecticides. Fueled this expectation, article reviews current knowledge about pathways insects, other questions such delivery recombinant RNA, critical establish reliable technology insect control plants.

Язык: Английский

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RNAi-based biocontrol for crops: a revised expectation for a non-recent technology

Planta, Год журнала: 2025, Номер 261(2)

Опубликована: Янв. 25, 2025

Язык: Английский

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Emerald Ash Borer Management and Research: Decades of Damage and Still Expanding

Annual Review of Entomology, Год журнала: 2023, Номер 69(1), С. 239 - 258

Опубликована: Сен. 14, 2023

Since the discovery of ash tree ( Fraxinus spp.) killer emerald borer (EAB; Agrilus planipennis) in United States 2002 and Moscow, Russia 2003, substantial detection management efforts have been applied to contain monitor its spread mitigate impacts. Despite these efforts, pest continues within North America. It has European Ukraine is causing sporadic outbreaks native range China. The dynamics EAB's expansion events appear be linked lack resistant trees invaded ranges, facilitated by abundance or planted American susceptible species. We review recently gained knowledge EAB; ecological, economic, social impacts; past with their successes limitations. also highlight advances biological control, mechanisms resistance, new approaches under development, aim guiding more effective management.

Язык: Английский

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RNA Interference-Based Forest Protection Products (FPPs) Against Wood-Boring Coleopterans: Hope or Hype?

Frontiers in Plant Science, Год журнала: 2021, Номер 12

Опубликована: Сен. 10, 2021

Graphical Abstract Three-tier impact of forest protection products (FPPs).

Язык: Английский

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Oral Ingestion of Bacterially Expressed dsRNA Can Silence Genes and Cause Mortality in a Highly Invasive, Tree-Killing Pest, the Emerald Ash Borer

Insects, Год журнала: 2020, Номер 11(7), С. 440 - 440

Опубликована: Июль 14, 2020

RNA interference (RNAi) is a naturally occurring process inhibiting gene expression, and recent advances in our understanding of the mechanism have allowed its development as tool against insect pests. A major challenge for deployment field convenient efficient methods production double stranded (dsRNA). We assessed potential deploying bacterially produced dsRNA bio-pesticide an invasive forest pest, emerald ash borer (EAB). EAB feeds on cambial tissue trees (Fraxinus spp.), causing rapid death. has killed millions North America since discovery 2002, prompting need innovative management strategies. In study, bacterial expression synthesis were performed with E. coli strain HT115 using L4440 vector. EAB-specific dsRNAs (shi hsp) over-expressed toxic to neonate after oral administration, successfully triggering silencing subsequent mortality; however, non-specific control was not included. Our results suggest that ingestion transformed expressing can induce RNAi response EAB. To knowledge, this first example effective induced by feeding dsRNA-expressing bacteria pest.

Язык: Английский

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Southern pine beetle-specific RNA interference exhibits no effect on model nontarget insects

Journal of Pest Science, Год журнала: 2022, Номер 95(3), С. 1429 - 1441

Опубликована: Янв. 4, 2022

Abstract The efficacy and high specificity of the RNA interference pathway has prompted its exploration as a potential molecular management tool for many insect pests, including destructive southern pine beetle, Dendroctonus frontalis Zimmermann, in which gene knockdown mortality via double-stranded RNAs (dsRNAs) have already been demonstrated laboratory. nucleotide sequence dsRNAs requires an exact match at least 16 nucleotides with targeted messenger to trigger that gene. This allows vital genes target pest be silenced induced while reducing probability adverse effects nontarget organisms. However, prior utilization forest ecosystems, demonstration through laboratory bioassays evaluating on model insects is required proper risk assessment analyses. Consequently, we evaluated three SPB-specific lethal effects, sublethal (larval growth rate, adult emergence or fecundity), relative expression representing key functional guilds, predator, herbivore, pollinator. had no effect survival our insects. Additionally, were found analyses corroborated bioinformatic finding knockdown. Our findings support RNAi technology provide development deployment protection conifer forests against SPB minimal concerns.

Язык: Английский

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Exogenously Induced Silencing of Four MYB Transcription Repressor Genes and Activation of Anthocyanin Accumulation in Solanum lycopersicum

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(11), С. 9344 - 9344

Опубликована: Май 26, 2023

RNA interference (RNAi) is a natural post-transcriptional regulatory mechanism that can be artificially induced by exogenous application of double-stranded RNAs (dsRNAs) to the plant surfaces. Recent studies show it possible silence genes and change properties using spraying other approaches for dsRNA delivery. In this study, we investigated effect gene-specific dsRNAs on silencing four tomato encoding MYB-family transcription repressors anthocyanin biosynthesis in leaves Solanum lycopersicum L. We found SlMYBATV1, SlMYB32, SlMYB76, SlTRY downregulated mRNA levels these endogenous production, upregulated expression biosynthesis-related genes, enhanced content S. lycopersicum. The data demonstrated induce gene direct foliar dsRNAs. This approach may used secondary metabolism induction as tool function without need produce genetically modified plants.

Язык: Английский

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Feasibility of Systemically Applied dsRNAs for Pest-Specific RNAi-Induced Gene Silencing in White Oak

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Март 16, 2022

The efficacy of double-stranded RNA (dsRNA) in inducing host specific gene knockdown and mortality has been demonstrated a multitude insects dsRNAs are being integrated for pest suppression variety agricultural horticultural crops. However, less attention applied to their use forest settings, despite the susceptibility multiple pests RNAi. Prior implementation suppression, characterization specificity, efficacy, behavior environment is essential. Therefore, we investigated translocation retention exogenously dsRNA an economically ecologically significant hardwood tree when hydroponically. White oak (Quercus alba, L.) seedlings were exposed as root soak, at 1, 3, 5, 7 days post-exposure destructively sampled, divided into stem leaf tissue, extracted. Gel electrophoresis was used visualize presence exogenous treated seedling material Sanger sequencing further verify recovery treatment dsRNAs. Both techniques confirmed each tissue type sample interval, demonstrating successful uptake through white tissues. Our findings support viable delivery method seedlings, which could provide single protection from selected feeding or pathogens.

Язык: Английский

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Foliar Application of dsRNA to Induce Gene Silencing in Emerald Ash Borer: Systemic Distribution, Persistence, and Bioactivity

Forests, Год журнала: 2023, Номер 14(9), С. 1853 - 1853

Опубликована: Сен. 12, 2023

RNA interference (RNAi) is a cellular mechanism triggered by double-stranded (dsRNA), in which gene expression reduced sequence-specific manner, allowing development of pest-specific control strategies. Effective delivery the dsRNA hurdle, particularly systems with endophagous insects such as emerald ash borer (EAB) Agrilus planipennis, an invasive phloem-feeding beetle that develops beneath bark trees, Fraxinus spp., causing rapid tree death. We evaluated uptake and bioactivity green (F. pennsylvanica) growing greenhouse to investigate feasibility through host plant. To assess persistence distribution, seedlings were exposed EAB-specific using foliar spray sectioned into different tissue types at selected time-points; RT-PCR Sanger sequencing used detect exogenous dsRNA. found plant tissues 21 days after treatment. evaluate bioactivity, neonate EAB treated assessed for feeding behavior. Results demonstrate silencing 24% (p = 0.03) reduction cambial consumption. Our findings provide proof-of-concept RNAi target insect plant, suggesting functioning sustainable approach protection against EAB.

Язык: Английский

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Advances on polymeric nanocarriers for sustainable agriculture: Enhancing dsRNA/siRNA delivery to combat agricultural pests

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 282, С. 137000 - 137000

Опубликована: Окт. 30, 2024

Язык: Английский

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