European Journal of Wildlife Research,
Год журнала:
2024,
Номер
70(4)
Опубликована: Июль 25, 2024
Abstract
Climatic
variation
along
the
elevation
gradient
promotes
natural
parapatric
occurrence
of
European
hare
(
Lepus
europaeus
)
and
Alpine
mountain
timidus
varronis
in
Alps.
Recent
data
indicate
a
displacement
hares
caused
by
competition
with
hare.
Competitive
exclusion
might
take
place
at
fine
spatial
scale
hybrids
may
sharpen
competition.
Genetic
non-invasive
sampling
(gNIS)
demonstrates
to
be
effective
retrieve
information
from
wild
animals.
However,
based
on
accuracy
differing
genetic
analysis
methods,
selection
method
decisively
influence
results.
To
examine
habitat
preferences
hares,
their
particular
interest
results,
we
performed
gNIS
Grisons
(Switzerland)
for
four
years
compared
associations
genotyped
samples.
We
recorded
137
individuals
(i.e.,
35
hybrids,
49
53
hares).
Combined
nuclear
mitochondrial
DNA
including
individual
identification
revealed
most
accurate
indirect
study
hares.
had
narrow
breadth
used
little
diversity.
Hybrids
showed
great
similarities
increase
favour
since
they
show
similar
patterns
use
Ongoing
climate
change
potentiate
niche
overlap
between
species,
increasing
risk
decline
due
hybridisation
displacement.
Ecology and Evolution,
Год журнала:
2021,
Номер
12(1)
Опубликована: Дек. 27, 2021
Abstract
Effective
conservation
requires
accurate
data
on
population
genetic
diversity,
inbreeding,
and
structure.
Increasingly,
scientists
are
adopting
non‐invasive
sampling
(gNIS)
as
a
cost‐effective
population‐wide
monitoring
approach.
gNIS
has,
however,
known
limitations
which
may
impact
the
accuracy
of
downstream
analyses.
Here,
using
high‐quality
single
nucleotide
polymorphism
(SNP)
from
blood/tissue
free‐ranging
koala
(
n
=
430),
we
investigated
how
reduced
SNP
panel
size
call
rate
typical
samples
(derived
experimental
field
trials)
impacts
measures,
also
effect
intensity
these
measures.
We
found
that
at
small
sample
sizes
(14%
population)
can
provide
diversity
but
slightly
underestimated
inbreeding
coefficients.
Accurate
measures
internal
relatedness
required
least
33%
to
be
sampled.
geographic
spatial
autocorrelation
analysis
between
28%
51%
show
low
powerful
tool
aid
decision‐making
recommendations
for
researchers
looking
apply
techniques
systems.
Mammalian Biology,
Год журнала:
2022,
Номер
102(3), С. 863 - 874
Опубликована: Фев. 24, 2022
Abstract
Non-invasive
genetic
sampling
can
facilitate
the
identification
of
individual
animals
across
a
landscape,
with
applications
to
management
and
conservation.
Fecal
material
is
readily
available
source
DNA,
various
methods
exist
for
collecting
fecal
samples
DNA
preservation.
In
particular,
swab
offer
considerable
promise,
but
their
utility
in
real-world
field
contexts
remains
relatively
untested.
We
systematically
compared
multiple
all
stages
data
collection
analysis,
including
field,
extraction
lab,
individuals
using
microsatellite
genotyping.
collected
112
from
black-tailed
deer
(
Odocoileus
hemionus
columbianus
)
Mendocino
County,
California,
range
sample
conditions
unknown
age.
efficiency,
ease,
genotyping
success
three
storage
ungulate
samples:
whole
pellets
ethanol,
dry
paper
envelopes,
cotton
swabs
buffer.
Storage
method,
condition,
interaction
predicted
top
binomial
GLMMs.
found
that
swabbing
resulted
greatest
percentage
individually
identifiable
genotypes
(81%,
60%
56%
ethanol),
despite
lower
concentrations.
While
requires
greater
time
investment
are
easier
safer
store
transport,
subsequent
labwork
more
efficient
as
whole-pellet
methods.
We,
therefore,
recommend
method
most
contexts.
provide
additional
recommendations
protocols
based
on
2284
larger
monitoring
study
population,
given
this
large
number
spanned
spent
storage.
Conservation,
Год журнала:
2022,
Номер
2(1), С. 51 - 67
Опубликована: Янв. 10, 2022
Understanding
how
small
mammals
(SM)
are
associated
with
environmental
characteristics
in
olive
groves
is
important
to
identify
potential
threats
agriculture
and
assess
the
overall
conservation
value
functioning
of
agro-ecosystems.
Here,
we
provide
first
insights
on
this
topic
applied
traditional
northeast
(NE)
Portugal
by
assessing
landscape
attributes
that
determine
SM
occurrence,
focusing
one
species
concern
(Microtus
cabrerae
Thomas
1906)
often
perceived
as
a
pest
olives
lusitanicus
Gerbe
1879).
Based
genetic
non-invasive
sampling
51
surrounding
habitats,
identified
seven
rodent
insectivore.
Occupancy
modelling
indicated
were
generally
less
detected
within
than
habitats.
The
vulnerable
M.
reached
mean
occupancy
(95%
CI)
0.77
(0.61–0.87),
while
stood
at
0.37
(0.24–0.52).
was
more
likely
occur
land
mosaics
high
density
agricultural
field
edges,
pastureland
patches.
Overall,
our
study
suggests
complex
structure
spatial
heterogeneity
traditionally
managed
grove
agro-ecosystems
may
favor
occurrence
species-rich
communities,
possibly
including
well-established
populations
importance,
keeping
relatively
low
rates.
European Journal of Wildlife Research,
Год журнала:
2024,
Номер
70(4)
Опубликована: Июль 25, 2024
Abstract
Climatic
variation
along
the
elevation
gradient
promotes
natural
parapatric
occurrence
of
European
hare
(
Lepus
europaeus
)
and
Alpine
mountain
timidus
varronis
in
Alps.
Recent
data
indicate
a
displacement
hares
caused
by
competition
with
hare.
Competitive
exclusion
might
take
place
at
fine
spatial
scale
hybrids
may
sharpen
competition.
Genetic
non-invasive
sampling
(gNIS)
demonstrates
to
be
effective
retrieve
information
from
wild
animals.
However,
based
on
accuracy
differing
genetic
analysis
methods,
selection
method
decisively
influence
results.
To
examine
habitat
preferences
hares,
their
particular
interest
results,
we
performed
gNIS
Grisons
(Switzerland)
for
four
years
compared
associations
genotyped
samples.
We
recorded
137
individuals
(i.e.,
35
hybrids,
49
53
hares).
Combined
nuclear
mitochondrial
DNA
including
individual
identification
revealed
most
accurate
indirect
study
hares.
had
narrow
breadth
used
little
diversity.
Hybrids
showed
great
similarities
increase
favour
since
they
show
similar
patterns
use
Ongoing
climate
change
potentiate
niche
overlap
between
species,
increasing
risk
decline
due
hybridisation
displacement.