Tissue engineering and future directions in regenerative medicine for knee cartilage repair: a comprehensive review DOI Creative Commons
Dragan Primorac, Vilim Molnar, Dimitrios Tsoukas

и другие.

Croatian Medical Journal, Год журнала: 2024, Номер 65(3), С. 268 - 288

Опубликована: Июнь 1, 2024

This review evaluates the current landscape and future directions of regenerative medicine for knee cartilage repair, with a particular focus on tissue engineering strategies. In this context, scaffold-based approaches have emerged as promising solutions regeneration. Synthetic scaffolds, while offering superior mechanical properties, often lack biological cues necessary effective integration. Natural though biocompatible biodegradable, frequently suffer from inadequate strength. Hybrid combining elements both synthetic natural materials, present balanced approach, enhancing support functionality. Advances in decellularized extracellular matrix scaffolds shown potential promoting cell infiltration integration native tissues. Additionally, bioprinting technologies enabled creation complex, bioactive that closely mimic zonal organization cartilage, providing an optimal environment growth differentiation. The also explores gene therapy editing techniques, including CRISPR-Cas9, to enhance repair by targeting specific genetic pathways involved these advanced therapies holds promise developing personalized durable treatments injuries osteoarthritis. conclusion, underscores importance continued multidisciplinary collaboration advance innovative bench bedside improve outcomes patients damage.

Язык: Английский

A 3D printed biomimetic scaffold for cartilage regeneration with lubrication, load-bearing, and adhesive fixation properties DOI
Pengyu Wei,

Yuanya Ma,

Kaiqi Qin

и другие.

Tribology International, Год журнала: 2024, Номер 192, С. 109328 - 109328

Опубликована: Янв. 24, 2024

Язык: Английский

Процитировано

6

Beyond the Surface: Nutritional Interventions Integrated with Diagnostic Imaging Tools to Target and Preserve Cartilage Integrity: A Narrative Review DOI Creative Commons
Salvatore Lavalle, Rosa Scapaticci,

Edoardo Masiello

и другие.

Biomedicines, Год журнала: 2025, Номер 13(3), С. 570 - 570

Опубликована: Фев. 24, 2025

This narrative review provides an overview of the various diagnostic tools used to assess cartilage health, with a focus on early detection, nutrition intervention, and management osteoarthritis. Early detection damage is crucial for effective patient management. Traditional like radiography conventional magnetic resonance imaging (MRI) sequences are more suited detecting late-stage structural changes. paper highlights advanced techniques, including sodium MRI, T2 mapping, T1ρ imaging, delayed gadolinium-enhanced MRI cartilage, which provide valuable biochemical information about composition, particularly glycosaminoglycan content its potential links nutrition-related factors influencing health. Cartilage degradation often linked inflammation measurable via markers CRP IL-6 which, although not specific breakdown, offer insights into affecting cartilage. In addition markers, such as collagen breakdown products aggrecan fragments, reflect metabolic changes in discussed. Emerging optical coherence tomography hybrid positron emission tomography–magnetic (PET-MRI) also explored, offering high-resolution combined insights, respectively. Finally, wearable technology biosensors real-time monitoring osteoarthritis progression, well role artificial intelligence enhancing accuracy through pattern recognition data addressed. While these hold great osteoarthritis, challenges remain clinical translation, validation larger populations integration existing workflows personalized treatment strategies cartilage-related diseases.

Язык: Английский

Процитировано

0

Free glutaraldehyde gelatin microsphere loaded mesenchymal stem cells alleviate osteoarthritis by promoting Ext1 expression DOI Creative Commons
Ye Yuan, Longsheng Xu, Yu Zhao

и другие.

Theranostics, Год журнала: 2025, Номер 15(9), С. 4135 - 4146

Опубликована: Март 10, 2025

Rationale: Osteoarthritis (OA) is a chronic joint disorder with limited treatment efficacy, necessitating innovative therapeutic strategies. This study explores one-pot-synthesized gelatin microspheres devoid of glutaraldehyde as novel biomaterial for OA management. Focusing on the Ext1 gene, critical cartilage development and downregulated in OA, we investigated its restoration immune regulation using cultured mesenchymal stem cells (MSCs). Methods: patients undergoing knee replacement surgery have their lateral compartment (remote zone) medial (lesion collected transcriptomic testing. The differential gene identified, expression regulatory genes examined. MSCs were to evaluate compatibility ability promote cell attachment. effects overexpression, regulation, symptom mitigation through vitro vivo experiments. Results: exhibit decreased area, accompanied by abnormal genes. demonstrated that exhibited excellent facilitated Culturing led enhanced overexpression which crucial growth development. Additionally, regulated responses, contributing reduction symptoms. Conclusion: introduces an strategy osteoarthritis MSCs. By promoting regulating these effectively mitigate findings highlight potential this promising option OA.

Язык: Английский

Процитировано

0

Surface slicing and toolpath planning for in-situ bioprinting of skin implants DOI Creative Commons
Muhammad Salman Chaudhry, Aleksander Czekanski

Biofabrication, Год журнала: 2024, Номер 16(2), С. 025030 - 025030

Опубликована: Март 6, 2024

Abstract Bioprinting has emerged as a successful method for fabricating engineered tissue implants, offering great potential wound healing applications. This study focuses on an advanced surface-based slicing approach aimed at designing skin implant specifically in-situ bioprinting. The step plays crucial role in determining the layering arrangement of during printing. By utilizing surface slicing, significant shift from planar fabrication methods is achieved. developed methodology involves utilization customized robotic printer to deliver biomaterials. A multilayer and toolpath generation procedure presented, enabling implants that incorporate epidermal, dermal, hypodermal layers. One notable advantage using approximate representation native site avoidance printing effects such staircasing. allows design non-planar ultra-thin ensuring higher degree geometric match between interface. Furthermore, proposed demonstrates superior quality bio-printed hand model, validating its ability create toolpaths with complex surfaces.

Язык: Английский

Процитировано

1

Tissue engineering and future directions in regenerative medicine for knee cartilage repair: a comprehensive review DOI Creative Commons
Dragan Primorac, Vilim Molnar, Dimitrios Tsoukas

и другие.

Croatian Medical Journal, Год журнала: 2024, Номер 65(3), С. 268 - 288

Опубликована: Июнь 1, 2024

This review evaluates the current landscape and future directions of regenerative medicine for knee cartilage repair, with a particular focus on tissue engineering strategies. In this context, scaffold-based approaches have emerged as promising solutions regeneration. Synthetic scaffolds, while offering superior mechanical properties, often lack biological cues necessary effective integration. Natural though biocompatible biodegradable, frequently suffer from inadequate strength. Hybrid combining elements both synthetic natural materials, present balanced approach, enhancing support functionality. Advances in decellularized extracellular matrix scaffolds shown potential promoting cell infiltration integration native tissues. Additionally, bioprinting technologies enabled creation complex, bioactive that closely mimic zonal organization cartilage, providing an optimal environment growth differentiation. The also explores gene therapy editing techniques, including CRISPR-Cas9, to enhance repair by targeting specific genetic pathways involved these advanced therapies holds promise developing personalized durable treatments injuries osteoarthritis. conclusion, underscores importance continued multidisciplinary collaboration advance innovative bench bedside improve outcomes patients damage.

Язык: Английский

Процитировано

0