Optimization of Gelatin and Crosslinker Concentrations in a Gelatin/Alginate-Based Bioink with Potential Applications in a Simplified Skin Model
Molecules,
Год журнала:
2025,
Номер
30(3), С. 649 - 649
Опубликована: Фев. 1, 2025
Three-dimensional
bioprinting
allows
for
the
fabrication
of
structures
mimicking
tissue
architecture.
This
study
aimed
to
develop
a
gelatin-based
bioink
bioprinted
simplified
skin
model.
The
printability
and
chemical-physical
properties
were
evaluated
by
varying
concentrations
gelatin
(10,
15,
20%)
in
semi-crosslinked
alginate-based
calcium
chloride
(100,
150,
200
mM)
post-printing
crosslinking.
For
increasing
concentration,
formulations
have
shear
thinning
behavior
with
viscosity,
filament
bending
angle
increases,
spreading
ratio
value
approaches
1,
shape
fidelity
printing
resolution
improve.
However,
formulation
containing
20%
was
not
homogeneous,
resulting
also
poor
properties.
morphology
pores,
degradation,
swelling
depend
on
CaCl2
concentrations,
but
significant
way.
samples
15%
crosslinked
150
mM
been
selected
bilayer
model
human
fibroblasts
keratinocytes.
showed
homogeneous
distribution
viable
proliferating
cells
over
up
14
days
vitro
culture.
allowed
3D
model,
potential
applications
bioactivity
pro-reparative
molecules
drug
evaluation.
Язык: Английский
“Lost in translation?” Animal research in the era of precision medicine
Journal of Translational Medicine,
Год журнала:
2025,
Номер
23(1)
Опубликована: Фев. 4, 2025
Abstract
Preclinical
animal
research
has
long
been
a
cornerstone
in
evaluating
the
efficacy,
toxicity,
and
safety
of
potential
drug
treatments
before
they
proceed
to
human
clinical
trials.
However,
given
intricate
nature
physiology
complexities
diseases
such
as
cancer,
this
paper
critically
examines
role
experimentation
translational
research,
both
from
epistemological
ethical
viewpoints.
We
argue
that
obligation
protect
animals
extends
beyond
their
instrumental
value
for
benefit;
it
is
rooted
intrinsic
well-being.
Consequently,
we
advocate
paradigm
shift
medical
research:
adoption
new
approach
methodologies
(NAMs)
not
merely
supplementary
tools
but
complete
replacements
use
studies.
In
context,
replacement
emerges
key
principle—an
imperative
should
be
prioritized
over
all
other
considerations.
Язык: Английский
Potential of 3D Bioprinting Solutions Towards Revolutionizing Healthcare
IGI Global eBooks,
Год журнала:
2025,
Номер
unknown, С. 213 - 232
Опубликована: Май 13, 2025
The
field
of
3D
bioprinting
is
revolutionizing
healthcare
by
enabling
the
creation
biological
tissues
and
organs
with
unprecedented
precision
customization.
As
an
advanced
extension
additive
manufacturing,
incorporates
living
cells,
bioinks,
growth
factors
to
fabricate
constructs
that
mimic
natural
in
structure
function.
This
chapter
explores
transformative
potential
across
key
domains,
including
regenerative
medicine,
pharmaceutical
development,
personalized
treatments,
surgical
planning,
medical
education.
Bioprinting's
ability
address
critical
challenges—such
as
organ
shortages,
inefficiencies
drug
testing,
growing
need
for
tailored
solutions—positions
it
a
cornerstone
modern
medicine.
provides
comprehensive
overview
technology,
detailing
state-of-the-art
techniques
such
inkjet
printing,
extrusion-based
laser-assisted
bioprinting,
alongside
advancements
bioink
development.
Язык: Английский
Effective and new technologies in kidney tissue engineering
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Окт. 16, 2024
Kidney
disease
encompasses
a
wide
spectrum
of
conditions,
ranging
from
simple
infections
to
chronic
kidney
disease.
When
the
kidneys
are
unable
filter
blood
and
remove
waste
products,
these
abnormalities
can
lead
failure.
In
severe
cases
failure,
transplantation
is
considered
only
definitive
treatment.
Worldwide,
World
Health
Organization
(WHO)
repeatedly
emphasizes
importance
organ
donation
increasing
rates.
Many
countries
implement
national
programs
promote
culture
improve
patient
access
transplantation.
The
extent
which
this
procedure
performed
varies
across
influenced
by
several
factors,
including
volume
donation,
medical
infrastructure,
technology
health
policies.
However,
transplant
comes
with
challenges
problems
that
impact
its
success.
tissue
engineering
new
approach
shows
promise
for
repairing
replacing
damaged
tissue.
This
article
reviews
recent
advances
in
engineering,
focusing
on
engineered
structures
such
as
hydrogels,
electrospinning,
3D
bioprinting,
microfluidic
systems.
By
mimicking
extracellular
environment
kidney,
provide
suitable
conditions
growth
development
cells.
role
formation
vessels,
mimicry
functions
field
were
also
discussed.
results
study
show
has
high
potential
treating
diseases
reducing
need
achieve
clinical
application
technology,
further
research
required
biocompatibility,
vascularization
long-term
performance
tissues.
Язык: Английский
3-D-Druck in der Chirurgie: Relevanz der Bewertung der Technologiereife in Forschungsstudien zum Bioprinting
Deleted Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 4, 2024
Zusammenfassung
Biologische
3‑D-Druckverfahren
(sog.
Bioprinting)
sind
eine
Erweiterung
dessen,
was
in
den
American
Society
for
Testing
and
Materials(ASTM)-
und
International
Organization
Standardization(ISO)-Normen
als
additive
Fertigung
definiert
ist,
basieren
auf
dem
automatisierten
Druck
von
lebenden
Zellen
Biomaterialien.
Forschende
Expertinnen
Experten
im
Bereich
der
Biomaterialwissenschaften,
Gewebezüchtung
regenerativen
Medizin
(„tissue
engineering
regenerative
medicine“,
TE&RM)
verweisen
stets
das
Potenzial
biologischer
Fachartikeln
wird
regelmäßig
dessen
baldige
klinische
Anwendung
angekündigt.
Wir
argumentieren
dieser
Arbeit,
dass
diese
Ankündigungen
regelhaft
verfrüht
kontraproduktiv
sind,
da
sie
sich
stark
technologischen
Fortschritt
konzentrieren,
jedoch
Regel
die
kritischen
Phasen
ignorieren,
durchlaufen
werden
müssen,
um
erfolgreich
Translation
einer
Technologie
Gesundheitsmarkt
zu
erzielen.
Die
Technologiereifegradskala
(„technology
readiness
level“,
TRL)
ist
ein
potenziell
nützliches
Instrument
zur
Messung
relativen
Reife
Bezug
Überwindung
Reihe
kritischer
Meilensteine.
schlagen
Adaptierung
TRL-Skala
vor
nutzen
diese,
aktuellen
Stand
Forschung
biologischen
diskutieren.
Abschließend
geben
wir
konkrete
Empfehlungen
Optimierung
zukünftiger
Forschungsprojekte,
Weg
für
Anwendungen
des
3‑D-Drucks
ebnen
damit
einen
direkten
positiven
Einfluss
chirurgische
Patientenversorgung