Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging DOI
Geng Yang, Ting Song, Meng Wang

и другие.

ACS Applied Nano Materials, Год журнала: 2022, Номер 5(3), С. 3065 - 3086

Опубликована: Март 10, 2022

As one of the two nucleic acids used by extant biochemistry, RNA is great importance for cellular function and development, its expression can affect cell homeostasis. Therefore, accurate detection key contributor to disease diagnosis, including tumor detection. Compared with conventional methods, molecular beacons (MBs) have high selectivity target molecules detection, which been widely in However, background fluorescence typical MBs will seriously sensitivity accuracy. In addition, poor affinity weak resistance nuclease digestion also limit applications. With development nanotechnology, many nanomaterials quenching ability as quenchers carriers construct nanoprobes (Nano-MBs). this review, we summarize recent developments Nano-MBs imaging over past decade. First, structure, working principle, classification are discussed. Then, introduce several based on gold nanoparticles, polydopamine, graphene oxide, molybdenum disulfide, quantum dots. Particular emphasis placed application mRNA miRNA diagnosis treatment diseases, well cancer. Finally, current challenges future perspectives

Язык: Английский

MicroRNAs, DNA damage response and ageing DOI
Maryam Majidinia, Seyed Mostafa Mir, Mohammad Mirza‐Aghazadeh‐Attari

и другие.

Biogerontology, Год журнала: 2020, Номер 21(3), С. 275 - 291

Опубликована: Фев. 18, 2020

Язык: Английский

Процитировано

34

scEpiAge: an age predictor highlighting single-cell ageing heterogeneity in mouse blood DOI Creative Commons
Marc Jan Bonder, Stephen J. Clark, Felix Krueger

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 31, 2024

Abstract Ageing is the accumulation of changes and decline function organisms over time. The concept biomarkers biological age have been established, notably DNA methylation-based clocks. emergence single-cell methylation profiling methods opens possibility studying individual cells. Here, we generate a large transcriptome dataset from mouse peripheral blood samples, spanning broad range ages. number genes expressed increases with age, but gene-specific are small. We next develop scEpiAge, predictor, which can accurately predict in (very sparse) publicly available datasets, also single distribution wider than technically expected, indicating epigenetic heterogeneity functional differences. Our work provides foundation for sparse data predictors, validates their functionality highlights during ageing.

Язык: Английский

Процитировано

5

Transposable elements, circular RNAs and mitochondrial transcription in age-related genomic regulation DOI Open Access
Juan I. Bravo,

Séverine Nozownik,

Prakroothi S. Danthi

и другие.

Development, Год журнала: 2020, Номер 147(11)

Опубликована: Июнь 1, 2020

ABSTRACT Our understanding of the molecular regulation aging and age-related diseases is still in its infancy, requiring in-depth characterization landscape shaping these complex phenotypes. Emerging classes molecules with promise as modulators include transposable elements, circRNAs mitochondrial transcriptome. Analytical complexity means that are often overlooked, even though they exhibit strong associations and, some cases, may directly contribute to progress. Here, we review links between novel factors phenotypes, suggest tools can be easily incorporated into existing pipelines better understand process.

Язык: Английский

Процитировано

31

Transcriptome analysis reveals the difference between “healthy” and “common” aging and their connection with age‐related diseases DOI Creative Commons
Lu Zeng, Jialiang Yang, Shouneng Peng

и другие.

Aging Cell, Год журнала: 2020, Номер 19(3)

Опубликована: Фев. 19, 2020

Abstract A key goal of aging research was to understand mechanisms underlying healthy and develop methods promote the human healthspan. One approach is identify gene regulations unique compared with in general population (i.e., “common” aging). Here, we leveraged Genotype‐Tissue Expression (GTEx) project data investigate “healthy” expression at a tissue level humans their interconnection diseases. Using GTEx donors' disease annotations, defined cohort for each tissue. We then age‐associated genes derived from this which included all donors; also expressions various disease‐associated elucidate relationships among “healthy,” disease. Our analyses showed that 1. shared large number regulations; 2. Despite substantial commonality, distinct function enrichment, had higher enrichment genes; 3. Disease‐associated were overall different regulations. However, regulated by both, regulation directions largely consistent, implying some processes could increase susceptibility development; 4. Possible protective associated In summary, our work highlights several features program. This new knowledge potentially be used interventions

Язык: Английский

Процитировано

28

Recent Advancements in Nanosystem-Based Molecular Beacons for RNA Detection and Imaging DOI
Geng Yang, Ting Song, Meng Wang

и другие.

ACS Applied Nano Materials, Год журнала: 2022, Номер 5(3), С. 3065 - 3086

Опубликована: Март 10, 2022

As one of the two nucleic acids used by extant biochemistry, RNA is great importance for cellular function and development, its expression can affect cell homeostasis. Therefore, accurate detection key contributor to disease diagnosis, including tumor detection. Compared with conventional methods, molecular beacons (MBs) have high selectivity target molecules detection, which been widely in However, background fluorescence typical MBs will seriously sensitivity accuracy. In addition, poor affinity weak resistance nuclease digestion also limit applications. With development nanotechnology, many nanomaterials quenching ability as quenchers carriers construct nanoprobes (Nano-MBs). this review, we summarize recent developments Nano-MBs imaging over past decade. First, structure, working principle, classification are discussed. Then, introduce several based on gold nanoparticles, polydopamine, graphene oxide, molybdenum disulfide, quantum dots. Particular emphasis placed application mRNA miRNA diagnosis treatment diseases, well cancer. Finally, current challenges future perspectives

Язык: Английский

Процитировано

17