ACS Applied Nano Materials,
Год журнала:
2022,
Номер
5(3), С. 3065 - 3086
Опубликована: Март 10, 2022
As
one
of
the
two
nucleic
acids
used
by
extant
biochemistry,
RNA
is
great
importance
for
cellular
function
and
development,
its
expression
can
affect
cell
homeostasis.
Therefore,
accurate
detection
key
contributor
to
disease
diagnosis,
including
tumor
detection.
Compared
with
conventional
methods,
molecular
beacons
(MBs)
have
high
selectivity
target
molecules
detection,
which
been
widely
in
However,
background
fluorescence
typical
MBs
will
seriously
sensitivity
accuracy.
In
addition,
poor
affinity
weak
resistance
nuclease
digestion
also
limit
applications.
With
development
nanotechnology,
many
nanomaterials
quenching
ability
as
quenchers
carriers
construct
nanoprobes
(Nano-MBs).
this
review,
we
summarize
recent
developments
Nano-MBs
imaging
over
past
decade.
First,
structure,
working
principle,
classification
are
discussed.
Then,
introduce
several
based
on
gold
nanoparticles,
polydopamine,
graphene
oxide,
molybdenum
disulfide,
quantum
dots.
Particular
emphasis
placed
application
mRNA
miRNA
diagnosis
treatment
diseases,
well
cancer.
Finally,
current
challenges
future
perspectives
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 31, 2024
Abstract
Ageing
is
the
accumulation
of
changes
and
decline
function
organisms
over
time.
The
concept
biomarkers
biological
age
have
been
established,
notably
DNA
methylation-based
clocks.
emergence
single-cell
methylation
profiling
methods
opens
possibility
studying
individual
cells.
Here,
we
generate
a
large
transcriptome
dataset
from
mouse
peripheral
blood
samples,
spanning
broad
range
ages.
number
genes
expressed
increases
with
age,
but
gene-specific
are
small.
We
next
develop
scEpiAge,
predictor,
which
can
accurately
predict
in
(very
sparse)
publicly
available
datasets,
also
single
distribution
wider
than
technically
expected,
indicating
epigenetic
heterogeneity
functional
differences.
Our
work
provides
foundation
for
sparse
data
predictors,
validates
their
functionality
highlights
during
ageing.
ABSTRACT
Our
understanding
of
the
molecular
regulation
aging
and
age-related
diseases
is
still
in
its
infancy,
requiring
in-depth
characterization
landscape
shaping
these
complex
phenotypes.
Emerging
classes
molecules
with
promise
as
modulators
include
transposable
elements,
circRNAs
mitochondrial
transcriptome.
Analytical
complexity
means
that
are
often
overlooked,
even
though
they
exhibit
strong
associations
and,
some
cases,
may
directly
contribute
to
progress.
Here,
we
review
links
between
novel
factors
phenotypes,
suggest
tools
can
be
easily
incorporated
into
existing
pipelines
better
understand
process.
Abstract
A
key
goal
of
aging
research
was
to
understand
mechanisms
underlying
healthy
and
develop
methods
promote
the
human
healthspan.
One
approach
is
identify
gene
regulations
unique
compared
with
in
general
population
(i.e.,
“common”
aging).
Here,
we
leveraged
Genotype‐Tissue
Expression
(GTEx)
project
data
investigate
“healthy”
expression
at
a
tissue
level
humans
their
interconnection
diseases.
Using
GTEx
donors'
disease
annotations,
defined
cohort
for
each
tissue.
We
then
age‐associated
genes
derived
from
this
which
included
all
donors;
also
expressions
various
disease‐associated
elucidate
relationships
among
“healthy,”
disease.
Our
analyses
showed
that
1.
shared
large
number
regulations;
2.
Despite
substantial
commonality,
distinct
function
enrichment,
had
higher
enrichment
genes;
3.
Disease‐associated
were
overall
different
regulations.
However,
regulated
by
both,
regulation
directions
largely
consistent,
implying
some
processes
could
increase
susceptibility
development;
4.
Possible
protective
associated
In
summary,
our
work
highlights
several
features
program.
This
new
knowledge
potentially
be
used
interventions
ACS Applied Nano Materials,
Год журнала:
2022,
Номер
5(3), С. 3065 - 3086
Опубликована: Март 10, 2022
As
one
of
the
two
nucleic
acids
used
by
extant
biochemistry,
RNA
is
great
importance
for
cellular
function
and
development,
its
expression
can
affect
cell
homeostasis.
Therefore,
accurate
detection
key
contributor
to
disease
diagnosis,
including
tumor
detection.
Compared
with
conventional
methods,
molecular
beacons
(MBs)
have
high
selectivity
target
molecules
detection,
which
been
widely
in
However,
background
fluorescence
typical
MBs
will
seriously
sensitivity
accuracy.
In
addition,
poor
affinity
weak
resistance
nuclease
digestion
also
limit
applications.
With
development
nanotechnology,
many
nanomaterials
quenching
ability
as
quenchers
carriers
construct
nanoprobes
(Nano-MBs).
this
review,
we
summarize
recent
developments
Nano-MBs
imaging
over
past
decade.
First,
structure,
working
principle,
classification
are
discussed.
Then,
introduce
several
based
on
gold
nanoparticles,
polydopamine,
graphene
oxide,
molybdenum
disulfide,
quantum
dots.
Particular
emphasis
placed
application
mRNA
miRNA
diagnosis
treatment
diseases,
well
cancer.
Finally,
current
challenges
future
perspectives
