PARASITOIDS AS BIOCONTROL AGENTS IN INDIA
Current Opinion in Insect Science,
Год журнала:
2024,
Номер
66, С. 101282 - 101282
Опубликована: Окт. 12, 2024
Язык: Английский
Modulation of response to braconid wasp venom by adipokinetic hormone in Drosophila melanogaster
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology,
Год журнала:
2024,
Номер
285, С. 110005 - 110005
Опубликована: Авг. 23, 2024
The
minute
wasp
Habrobracon
hebetor
venom
(HH
venom)
is
a
potent
cocktail
of
toxins
that
paralyzes
the
victim's
muscles
and
suppresses
humoral
cellular
immunity.
This
study
examined
effect
HH
on
specific
biochemical,
physiological,
ultrastructural
characteristics
thoracic
nervous
(CNS)
tissues
Drosophila
melanogaster
under
in
vitro
conditions.
Venom
treatment
modulated
activities
superoxide
dismutase
(SOD)
catalase
(CAT),
endogenous
Drome-AKH
level,
affected
relative
viability
cells.
Additionally,
it
reduced
expression
genes
related
to
immune
system
CNS,
including
Keap1,
Relish,
Nox,
Eiger,
Gadd45,
Domeless,
as
well
muscles,
except
for
Nox.
Besides,
led
deteriorative
changes
ultrastructure
muscle
cells,
particularly
affecting
mitochondria.
When
melanogaster-adipokinetic
hormone
(Drome-AKH)
were
applied
together,
effects
alone
often
modulated.
harmful
SOD
activity
was
relatively
returned
level
similar
control.
In
simultaneous
application
hormones
abolished
suppression
previously
reported
immune-related
(except
Gadd45),
whereas
this
only
true
Eiger.
restored
cell
structure
comparable
control
lessened
These
findings
suggest
general
body
response
D.
partial
defensive
role
process.
Язык: Английский
Selection of a δ-latroinsectotoxin fragment from Latrodectus tredecimguttatus venom for efficient bacterial expression
Plant Protection News,
Год журнала:
2024,
Номер
107(3), С. 130 - 136
Опубликована: Ноя. 25, 2024
Toxins
from
the
venom
of
predatory
and
parasitoid
arthropods,
such
as
scorpions,
spiders,
wasps,
are
used
promising
tools
in
plant
protection
against
insect
pests.
In
many
cases,
polyclonal
antibodies
can
be
created
to
study
a
single
molecule
laboratory,
which
allows
for
specific
methods
detection
purification.
present
study,
heterologous
expression
δ-latroinsectotoxin
black
widow
spider
Latrodectus
tredecimguttatus,
well
its
N-
C-
terminal
fragments,
was
carried
out
Escherichia
coli
cells
at
various
temperatures
aeration
conditions.
Regardless
conditions,
accumulation
recombinant
protein
large
quantities
bacterial
inclusion
bodies
observed
only
C-terminal
fragment
toxin.
The
high
efficiency
this
allowed
use
insoluble
fraction
homogenate
immunization
mice
production
δ-latroinsectotoxin.
antigen-binding
activity
immunoglobulins
confirmed
by
immunoblotting
synthesized
toxin
bacteria
using
vector
with
removed
leader
sequences.
obtained
detect
during
systems,
creation
genetically
modified
entomopathogenic
fungi
or
viruses.
Язык: Английский