IGF2BP1 promotes endometriosis by enhancing m6A modification stability of HMGB1

Journal of Obstetrics and Gynaecology Research, Год журнала: 2025, Номер 51(2)

Опубликована: Фев. 1, 2025

Abstract Background Endometriosis is a chronic inflammatory condition afflicting women of reproductive age. Our study aims to clarify the function and mechanism insulin‐like growth factor 2 mRNA‐binding protein 1 (IGF2BP1) high mobility group box (HMGB1) in endometriosis. Methods HMGB1 various N6‐methyladenosine (m6A) reader levels were assessed normal, eutopic, ectopic endometrial tissue, correlation analysis was conducted. The impact IGF2BP1 knockdown on endometriosis both vivo rat models vitro stromal cells (eESCs) using methods such as immunoblotting mRNA quantification. binding eESCs RIP‐PCR. Following transfection with sh‐IGF2BP1 oe‐HMGB1, expression HMGB1, well cell proliferation, invasion, migration abilities, measured eESCs. Results In elevated positively correlated. Inhibition reduced eESC migration, glucose intake. Meanwhile, PKM2, HK2 depressed. vivo, results consistent vitro. Additionally, experiments confirmed that inhibition resulted lesion spherical volume, weight, interstitial lesions. bound enhanced its stability by m6A modification. Conversely, when knocked down overexpressed, opposite those observed previously. Conclusion promotes progression enhancing modification HMGB1. This provides theoretical basis for identifying therapeutic targets

Язык: Английский

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Role of m6A Methylation-Modified lncRNA in Digestive Tract Tumors

Hans Journal of Biomedicine, Год журнала: 2025, Номер 15(01), С. 117 - 125

Опубликована: Янв. 1, 2025

Язык: Английский

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Mechanism of FBXW7 mRNA degradation mediated by circSMAD2 through METTL3-METTL14 m6A axis affects proliferation and invasion of endometrial stromal cells

Journal of Reproductive Immunology, Год журнала: 2025, Номер unknown, С. 104523 - 104523

Опубликована: Март 1, 2025

Язык: Английский

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RNA modifications in female reproductive physiology and disease: emerging roles and clinical implications

Human Reproduction Update, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Abstract BACKGROUND RNA modifications, collectively known as the epitranscriptome, represent third layer of gene regulation, influencing expression at transcriptional, post-transcriptional, and translational levels. RNA-modifying proteins (RMPs), including writers, erasers, readers, are responsible for depositing, removing, recognizing chemical modifications on molecules. These play a crucial role in linking molecular processes to cellular functions. Over past few decades, growing body laboratory evidence, alongside advances sequencing technologies, has uncovered connections between aberrant reproductive disorders, highlighting their emerging roles female fertility. Given rapid expansion epitranscriptomic research reproduction, comprehensive review is needed summarize broader impacts various rather than focusing individual alone. OBJECTIVE AND RATIONALE This aims elucidate progress understanding biology how dysregulations contribute infertility-related conditions, such polycystic ovary syndrome (PCOS), premature ovarian insufficiency (POI), endometriosis. Special focus will be given coding RNAs, particularly those linked fertility supported by solid evidence. The ultimate objective explore targeting RNA-modification machinery can lead development novel therapeutic interventions restoring SEARCH METHODS We conducted thorough peer-reviewed original articles reviews published over two decades using PubMed search engine. Keywords included terms related ‘N6-methyladenosine (m6A)’, ‘N4-acetylcytidine (ac4C)’, ‘adenosine-to-inosine (A–I) editing’, combined with ‘ovary’, ‘oocyte’, ‘embryo’. Additional relevant phrases were also utilized ensure coverage topic. OUTCOMES modification emerged transformative area biology, our epitranscriptome rapidly due significant high-throughput technologies. Regulatory correct deposition functional implementation modifications. Knockout animal models have identified broad, though still incomplete, list involved mammalian processes. include prevalent mRNA, m6A, well A–I editing, and, lesser extent, 5-methylcytosine (m5C) ac4C. regulatory mechanisms impact functions, folliculogenesis, oocyte maturation, fertilization, embryo development. Dysregulation may exacerbate POI, PCOS, Although clinical investigations early stages, show great promise diagnostic biomarkers targets, potential improve health outcomes. WIDER IMPLICATIONS explores relatively underexamined offering significantly advance biology. It underscores relevance disorders identifies biomarkers, RMP-targeted therapies, that could shape future decision-making personalized treatments. insights clinicians embryologists, presenting new avenues diagnosis medicine. REGISTRATION NUMBER N/A.

Язык: Английский

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Epigenetics of maternal-fetal interface immune microenvironment and placental related pregnancy complications

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 3, 2025

Epigenetic regulation of placental development and pregnancy-related disease processes has recently been a hot research topic. Implantation subsequent depend on carefully orchestrated interactions between fetal maternal tissues, involving delicate balance immune factors. regulation, which refers to altering gene expression function without changing the DNA sequence, is an essential regulatory process in cell biology. Several epigenetic modifications are known, such as methylation, histone modifications, non-coding RNA methylation. Recently, there increasing evidence that critical for microenvironment at maternal-fetal interface. In this review, we highlight recent advances role epigenetics interface placenta-associated pregnancy complications.

Язык: Английский

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Upregulation of sperm-associated antigen 5 expression in endometrial carcinoma was associated with poor prognosis and immune dysregulation, and promoted cell migration and invasion

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июнь 11, 2024

Abstract Sperm-associated antigen 5 (SPAG5) regulates cancer cell invasion and is involved in the progression of many cancers. However, role SPAG5 endometrial carcinoma (EC) still unknown. The purpose this study was to explore EC its potential molecular mechanism. UALCAN tool cBioPortal were used analyze expression alterations EC, respectively. OncoLnc for survival analysis. We analyzed effects on immune infiltration levels checkpoints. also overexpressed knocked down cells effect regulation migration, invasion, apoptosis, cycle cells. found that gene often mutated EC. High significantly associated with poor overall patients affected level TIME checkpoints lymphocyte activating 3 (LAG3) T immunoreceptor Ig ITIM domains (TIGIT) It may be immunotherapy response these patients. In vitro experiments showed promotes migration invasion. conclusion, lays foundation further understanding mechanisms involving contributes diagnosing managing disease.

Язык: Английский

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FOXF2 suppressed esophageal squamous cell carcinoma by reducing M2 TAMs via modulating RNF144A-FTO axis

International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113422 - 113422

Опубликована: Окт. 23, 2024

Язык: Английский

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