Degradation of polyethylene terephthalate (PET) plastics by wastewater bacteria engineered via conjugation

Microbial Biotechnology, Год журнала: 2024, Номер 17(9)

Опубликована: Сен. 1, 2024

Wastewater treatment plants are one of the major pathways for microplastics to enter environment. In general, contaminants global concern that pose risks ecosystems and human health. Here, we present a proof-of-concept reduction microplastic pollution emitted from wastewater plants: delivery recombinant DNA bacteria in enable degradation polyethylene terephthalate (PET). Using broad-host-range conjugative plasmid, enabled various bacterial species municipal sample express FAST-PETase, which was released into extracellular We found FAST-PETase purified some transconjugant isolates could degrade about 40% 0.25 mm thick commercial PET film within 4 days at 50°C. then demonstrated partial post-consumer product over 5-7 by exposure conditioned media isolates. These results have broad implications addressing plastic problem enabling environmental PET.

Язык: Английский

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Degradation of PET Plastics by Wastewater Bacteria Engineered via Conjugation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 8, 2024

Abstract Microplastics are contaminants of global concern that pose risks to ecosystems and human health. Focusing on PET plastics, we present a proof-of-concept for reduction microplastic pollution: in situ engineering bacteria wastewater degrade PET. Using broad-host-range conjugative plasmid, enabled various bacterial species from municipal sample express FAST-PETase, which was released into the extracellular environment. We found FAST-PETase purified some isolates could about 40% 0.25 mm thick film within four days at 50 °C. then demonstrate partial degradation post-consumer over 5-7 by exposure conditioned media isolates. These results have broad implications addressing plastic pollution problem enabling environmental plastics .

Язык: Английский

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Microbial remediation and deteriorated corrosion in marine oil pollution remediation engineering: A critical review

Marine Pollution Bulletin, Год журнала: 2024, Номер 209, С. 117051 - 117051

Опубликована: Окт. 10, 2024

Язык: Английский

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Combining gamma-radiation and bioaugmentation enhances wastewater’s quality for its reuse in agricultural purposes

Environmental Technology, Год журнала: 2024, Номер unknown, С. 1 - 11

Опубликована: Ноя. 28, 2024

The reuse of wastewater in agriculture can be environmentally beneficial due to its abundance nutrients that promote plant growth and soil fertility. However, effluents (WWE) are often considered sources dissemination bacteria, antibiotics, heavy metal resistance genes, pathogens. In this study, we employed a combination gamma irradiation bioaugmentation as strategy for WWE treatment. Gamma facilitates the elimination pathogens degradation complex organic matter, while utilises consortium microorganisms specialised sorption. Bacterial strains were isolated from soils irrigated with selected based on their tolerance Cd (0.2 g L−1), Pb (1 L−1) Cu (1.5 L−1). A composed Bacillus selenatarsenatis S53, thuringiensis S15, Staphylococcus edaphicus S107 was biosorption capacity, which evaluated after 24 h incubation gamma-irradiated (WWEI). treated then used pea (Pisum sativum L.) seeds germination over 9 days' period. bacterial successfully biosorbed 180, 8085, 125 µg g−1 dry weight Cd, Pb, Cu, respectively, when incubated WWEI. Seed imbibition bioaugmented WWEI (WWEIB) resulted significant increases radicle epicotyl elongation compared (+91.6% +123.7%, respectively). Additionally, there an improvement fresh biomass production seedlings hydrated WWEIB WWE. Overall, appears highly promising safe enhancing crop productivity by mitigating contaminant-induced stress.

Язык: Английский

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Molecular Approaches to Microbial Identification of Industrially Significant Strains

Опубликована: Янв. 1, 2024

Язык: Английский

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How bioaugmentation for pesticide removal influences the microbial community in biologically active sand filters

Chemosphere, Год журнала: 2024, Номер 363, С. 142956 - 142956

Опубликована: Июль 27, 2024

Removing pesticides from biological drinking water filters is challenging due to the difficulty in activating pesticide-degrading bacteria within filters. Bioaugmented can alter filter's microbiome, affecting its performance either positively or negatively, depending on used and their interaction with native microbes. We demonstrate that adding specific strains effectively remove recalcitrant pesticides, like metaldehyde, yielding compliance regulatory standards for an extended period. Our experiments revealed Sphingobium CMET-H strain was particularly effective, consistently reducing metaldehyde concentrations levels compliance, significantly outperforming Acinetobacter calcoaceticus E1. This success attributed superior acclimation distribution of filter bed, facilitating more efficient interactions degradation pesticide, even when present at lower population densities compared Furthermore, our study demonstrates addition impacts microbiome various depths, despite these making up less than 1% total microbial community. The sequence which are introduced influences system's ability degrade effectively. research shows potential carefully selected dosed bioaugmented improve pesticide removal capabilities filtration systems, while also highlighting dynamics between communities. Further investigation into optimizing bioaugmentation strategies suggested enhance resilience efficiency treatment systems against contamination.

Язык: Английский

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Bacterial Genetic Bioaugmentation in the Bioremediation of Soil Pollutants: A Mini Review

Опубликована: Апрель 2, 2024

Язык: Английский

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Bioaugmentation: a strategy for enhanced degradation of pesticides in biobed

Journal of Environmental Science and Health Part B, Год журнала: 2024, Номер 59(10), С. 654 - 662

Опубликована: Сен. 23, 2024

Biopurification system (BPS) or biobeds are low-cost for decontamination of on-farm generated pesticide waste. A biobed contains a mixture soil, lignocellulosic biomass and organic matter source (compost/peat) works on the principal retention in high matrix its subsequent degradation by microbes. Bioaugmentation, green technology, is defined as improvement degradative capacity augmenting specific microorganisms. During last 20 years, several studies have evaluated augmented with bacterial fungal species prominent microorganism include genus

Язык: Английский

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Electro-Fenton purification of floodwater with a poly(vinyl alcohol)-treated FeNi3@laser-induced 3D-graphene composite anode from Kraft paper

Chemical Engineering Journal, Год журнала: 2024, Номер 503, С. 158206 - 158206

Опубликована: Дек. 3, 2024

Язык: Английский

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Biofiltration as a sustainable approach for the treatment of hydrophobic volatile organic compounds: Improvement strategies and integrated systems

Journal of Water Process Engineering, Год журнала: 2024, Номер 69, С. 106726 - 106726

Опубликована: Дек. 10, 2024

Язык: Английский

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