Possible biocontrol of bacterial blight in pomegranate using native endophytic Bacillus spp. under field conditions DOI Creative Commons

N. Manjunatha,

Somnath S. Pokhare, Ruchi Agarrwal

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 11, 2024

Bacterial blight in pomegranate, caused by Xanthomonas citri pv. punicae (Xcp), is one of the most devastating diseases, leading to substantial economic losses pomegranate production. Methods for management production are scarce and not well established. To date, major control strategy targeting pathogen with antibiotics copper-based compounds. However, excessive use has resulted development antibiotic resistance field population Xcp. Hence, as a means eco-friendly sustainable bacterial blight, native endophytes was investigated under conditions current study. Endophytic bacteria were isolated from micro-propagated nodal explants identified Bacillus haynesii , B. tequilensis subtilis . They found produce volatiles that inhibited Xcp growth during vitro antibiosis assay. GC–MS-based volatile profiling revealed presence several bioactive compounds reported antimicrobial activities. These (CFU 10 8 /mL) then spray-inoculated on leaves 6-month-old plants polyhouse. induce ROS-scavenging enzymes such catalase peroxidase. This alteration manifestation host tissue colonization ROS scavenging mechanisms which colonize plants. Furthermore, two-season trials reduction disease index 47–68%, considerably higher than due chemical immune modulator (2-bromo-2-nitro-1, 3-propanediol) currently being recommended control. In addition, these also exhibited reduced sensitivity this modulator; thus, study advocates biocontrol agents either alone or part integrated management.

Язык: Английский

Possible biocontrol of bacterial blight in pomegranate using native endophytic Bacillus spp. under field conditions DOI Creative Commons

N. Manjunatha,

Somnath S. Pokhare, Ruchi Agarrwal

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 11, 2024

Bacterial blight in pomegranate, caused by Xanthomonas citri pv. punicae (Xcp), is one of the most devastating diseases, leading to substantial economic losses pomegranate production. Methods for management production are scarce and not well established. To date, major control strategy targeting pathogen with antibiotics copper-based compounds. However, excessive use has resulted development antibiotic resistance field population Xcp. Hence, as a means eco-friendly sustainable bacterial blight, native endophytes was investigated under conditions current study. Endophytic bacteria were isolated from micro-propagated nodal explants identified Bacillus haynesii , B. tequilensis subtilis . They found produce volatiles that inhibited Xcp growth during vitro antibiosis assay. GC–MS-based volatile profiling revealed presence several bioactive compounds reported antimicrobial activities. These (CFU 10 8 /mL) then spray-inoculated on leaves 6-month-old plants polyhouse. induce ROS-scavenging enzymes such catalase peroxidase. This alteration manifestation host tissue colonization ROS scavenging mechanisms which colonize plants. Furthermore, two-season trials reduction disease index 47–68%, considerably higher than due chemical immune modulator (2-bromo-2-nitro-1, 3-propanediol) currently being recommended control. In addition, these also exhibited reduced sensitivity this modulator; thus, study advocates biocontrol agents either alone or part integrated management.

Язык: Английский

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