Genetic Analysis of the Awn Length Gene in the Rice Chromosome Segment Substitution Line CSSL29 DOI Open Access
Zhengjie Wang, Jun Yang, Tao Huang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1436 - 1436

Опубликована: Фев. 8, 2025

Awn length is a significant agronomic trait in rice. To analyze the genetic mechanism of awn chromosome segment substitution line 29 (CSSL29) derived from 9311 (recipient) into Nipponbare (NIP, donor), an F2 segregated population was constructed (indica) and CSSL29. The candidate genes were analyzed using quantitative loci sequencing (QTL-seq), yeast two-hybrid assays, 3 k 10 rice databases. results indicated that segregating followed normal distribution, long-awn phenotype CSSL29 controlled by multiple genes. Through BSA data, major QTL qAWN4 associated with identified on 4, containing cloned gene An-2. Further investigation revealed presence An-1, both exhibiting additive effect regulation phenotype. Yeast experiments confirmed no interaction between An-2 suggesting not mediated through simple protein-to-protein binding. Population analysis allele artificially selected during domestication but did significantly differ indica japonica subspecies. These findings enhance our understanding rice, laying groundwork for future research this area.

Язык: Английский

Genetic Analysis of the Awn Length Gene in the Rice Chromosome Segment Substitution Line CSSL29 DOI Open Access
Zhengjie Wang, Jun Yang, Tao Huang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1436 - 1436

Опубликована: Фев. 8, 2025

Awn length is a significant agronomic trait in rice. To analyze the genetic mechanism of awn chromosome segment substitution line 29 (CSSL29) derived from 9311 (recipient) into Nipponbare (NIP, donor), an F2 segregated population was constructed (indica) and CSSL29. The candidate genes were analyzed using quantitative loci sequencing (QTL-seq), yeast two-hybrid assays, 3 k 10 rice databases. results indicated that segregating followed normal distribution, long-awn phenotype CSSL29 controlled by multiple genes. Through BSA data, major QTL qAWN4 associated with identified on 4, containing cloned gene An-2. Further investigation revealed presence An-1, both exhibiting additive effect regulation phenotype. Yeast experiments confirmed no interaction between An-2 suggesting not mediated through simple protein-to-protein binding. Population analysis allele artificially selected during domestication but did significantly differ indica japonica subspecies. These findings enhance our understanding rice, laying groundwork for future research this area.

Язык: Английский

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