Rainbow Trout (Oncorhynchus mykiss) Pre-Smolts Treated with 11-Deoxycorticosterone Regulate Liver Carbohydrate Metabolism and Gill Osmoregulation DOI Open Access
Rodrigo Zuloaga,

Luciano Ahumada-Langer,

Jorge E. Aedo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3725 - 3725

Опубликована: Апрель 15, 2025

Smoltification is stressful for salmonids, and cortisol one of the central endocrine regulators seawater adaptation. It has been established that plays both mineralocorticoid glucocorticoid functions by MR GR, respectively, since aldosterone hormone absent. Recently, investigations have proposed 11-deoxycorticosterone (DOC) precursor might support effects, but this mechanism remains unclear. Hence, we assessed early effects DOC on rainbow trout pre-smolts, key smoltification stage, via metabolic transcriptomic approaches. Thirty-six juveniles (~120 g) were treated 3 h with (1 mg/kg) and/or (eplerenone) or (mifepristone) receptor antagonists (n = 6 each group). decreased plasma glucose pyruvate increased phosphate liver glycogen. also downregulated carbohydrate metabolism-related genes in liver. Finally, gill RNA-seq analysis presented 1660 differentially expressed transcripts versus vehicle, 1022 eplerenone + 3324 mifepristone DOC. The enrichment mainly revealed upregulation ion transmembrane transport metabolism downregulation stress innate immune responses. This suggests a significant role osmoregulation pre-smolts through receptors. could contribute to improving animal welfare monitoring during featuring novel potential biomarkers.

Язык: Английский

Rainbow Trout (Oncorhynchus mykiss) Pre-Smolts Treated with 11-Deoxycorticosterone Regulate Liver Carbohydrate Metabolism and Gill Osmoregulation DOI Open Access
Rodrigo Zuloaga,

Luciano Ahumada-Langer,

Jorge E. Aedo

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3725 - 3725

Опубликована: Апрель 15, 2025

Smoltification is stressful for salmonids, and cortisol one of the central endocrine regulators seawater adaptation. It has been established that plays both mineralocorticoid glucocorticoid functions by MR GR, respectively, since aldosterone hormone absent. Recently, investigations have proposed 11-deoxycorticosterone (DOC) precursor might support effects, but this mechanism remains unclear. Hence, we assessed early effects DOC on rainbow trout pre-smolts, key smoltification stage, via metabolic transcriptomic approaches. Thirty-six juveniles (~120 g) were treated 3 h with (1 mg/kg) and/or (eplerenone) or (mifepristone) receptor antagonists (n = 6 each group). decreased plasma glucose pyruvate increased phosphate liver glycogen. also downregulated carbohydrate metabolism-related genes in liver. Finally, gill RNA-seq analysis presented 1660 differentially expressed transcripts versus vehicle, 1022 eplerenone + 3324 mifepristone DOC. The enrichment mainly revealed upregulation ion transmembrane transport metabolism downregulation stress innate immune responses. This suggests a significant role osmoregulation pre-smolts through receptors. could contribute to improving animal welfare monitoring during featuring novel potential biomarkers.

Язык: Английский

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