Peer Review,
Год журнала:
2023,
Номер
5(17), С. 303 - 317
Опубликована: Июль 26, 2023
Lectins
are
involved
in
a
range
of
biological
mechanisms
related
to
recognition
and
binding
carbohydrates.
This
ability
is
possible
due
the
presence
carbohydrate
domain
with
capacity
differentiate
between
various
oligosaccharides
without
modify
molecules
which
it
binds.
The
study
lectins,
inserted
field
Glycobiology,
started
Stillmark’s
work
castor
bean
extract
continues
until
today
use
advanced
techniques
such
as
robust
hemagglutination
assay
affinity
chromatography.
particularly
abundant
plants
where
seem
be
seed
maturation
or
dormancy,
plant
defense,
storage
material,
N2
fixation.
Furthermore,
lectin
show
immunomodulatory
effects
through
signal-transduction
system
consequent
cytokines
production,
research
founded
by
Ehrlich
his
studies
mice
immunization.
Here
we
focus
on
lectins
their
role
physiology,
immune
response,
structural
analysis.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Фев. 7, 2025
Affinity
labeling
of
biomacromolecules
is
vital
for
bioimaging
and
functional
studies.
However,
affinity
probes
recognizing
glycans
with
high
specificity
remain
scarce.
Here
we
report
the
development
glycan
recombinant
binders
(GRABs)
based
on
mutant
bacterial
sialidases,
which
are
enzymatically
inactive
but
preserve
stringent
sialoglycan
substrates.
By
mutating
a
key
catalytic
residue
Streptococcus
pneumoniae
neuraminidase
A
(SpNanA)
Ruminococcus
gnavus
H
(RgNanH),
develop
GRAB-Sia
GRAB-Sia3
total
sialoglycans
α2,3-sialosides,
respectively.
The
GRABs
exhibit
strict
substrate
linkage
specificity,
tetramerization
streptavidin
substantially
increases
their
avidity.
tetrameric
(tetra-GRABs)
effective
tools
probing
in
immunoblotting,
flow
cytometry,
immunoprecipitation,
fluorescence
imaging.
Furthermore,
multiplex
analysis
tetra-GRABs
uncovers
spatially
distinct
various
mouse
organs.
This
work
provides
versatile
toolkit
analyzing
sensitivity,
convenience.
Highly
specific
labels
scarce,
limiting
use
many
applications.
Here,
authors
sialidase
mutants,
sialoglycans.
Biomarker Research,
Год журнала:
2023,
Номер
11(1)
Опубликована: Сен. 29, 2023
Abstract
Extracellular
vesicles
(EVs)
have
considerable
potential
as
diagnostic,
prognostic,
and
therapeutic
agents,
in
large
part
because
molecular
patterns
on
the
EV
surface
betray
cell
of
origin
may
also
be
used
to
“target”
EVs
specific
cells.
Cancer
is
associated
with
alterations
cellular
glycosylation
patterns,
enriched
glycan
moieties.
Glycoconjugates
play
versatile
roles
cancer
including
modulating
immune
response,
affecting
tumor
behavior
site
metastasis
such,
paving
way
for
development
innovative
diagnostic
tools
novel
therapies.
Entities
that
recognize
glycans,
such
lectins,
thus
powerful
discover
detect
biomarkers.
Indeed,
past
decade
has
seen
a
constant
increase
number
published
articles
lectin-based
strategies
detection
glycans.
This
review
explores
cancer-related
applications.
Furthermore,
this
summarizes
lectins
methods
screening,
targeting,
separation,
possible
identification
improved
biomarkers
from
EVs.
Cells,
Год журнала:
2022,
Номер
11(3), С. 339 - 339
Опубликована: Янв. 20, 2022
Pathogenic
enveloped
viruses
are
covered
with
a
glycan
shield
that
provides
dual
function:
the
structures
contribute
to
virus
protection
as
well
host
cell
recognition.
The
three
classical
types
of
N-glycans,
in
particular
complex
glycans,
high-mannose
and
hybrid
together
some
O-glycans,
participate
Ebola
virus,
influenza
human
cytomegalovirus,
herpes
immunodeficiency
Lassa
MERS-CoV,
SARS-CoV,
SARS-CoV-2,
which
responsible
for
respiratory
syndromes.
glycans
linked
glycoproteins
occur
metastable
prefusion
on
surface
infectious
virions
such
gp120
HIV,
hemagglutinin
influenza,
or
spike
proteins
beta-coronaviruses.
Plant
lectins
different
carbohydrate-binding
specificities
and,
especially,
mannose-specific
from
Vicieae
tribe,
pea
lectin
lentil
lectin,
can
be
used
probes
targeting
because
their
specific
interaction
α1,6-fucosylated
core
Man3GlcNAc2,
predominantly
occurs
glycans.
Other
plant
Neu5Ac
specificity
GalNAc/T/Tn
also
serve
potential
often
sialylated
truncated
respectively,
abundantly
distributed
viruses.
biomedical
therapeutical
antiviral
drugs
is
discussed.
