Synlett,
Год журнала:
2023,
Номер
35(12), С. 1417 - 1422
Опубликована: Ноя. 1, 2023
Abstract
In
this
study,
a
synthetic
approach
is
reported
for
generating
hepta-branched
β-cyclodextrins
(CDs)
bearing
heterogeneous
carbohydrate
residues
at
their
primary
side
via
one-pot
process
with
simultaneous
click
chemistry
reaction.
The
reactions
were
performed
by
reacting
two
or
three
different
species
of
2-propynylated
glycosides
hepta-azide
functional
β-CD
various
reaction
molar
ratios.
2-Propynylated
acted
as
sources
embedded
into
β-CD.
generated
several
desired
derivatives
varying
densities
the
carbohydrates
in
process.
article
describes
effects
combination
and
ratios
reactions.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(9), С. 4905 - 4905
Опубликована: Апрель 30, 2024
Osteoarthritis
is
more
prevalent
than
any
other
form
of
arthritis
and
characterized
by
the
progressive
mechanical
deterioration
joints.
Glucosamine,
an
amino
monosaccharide,
has
been
used
for
over
fifty
years
as
a
dietary
supplement
to
alleviate
osteoarthritis-related
discomfort.
Silibinin,
extracted
from
milk
thistle,
modifies
degree
glycosylation
target
proteins,
making
it
essential
component
in
treatment
various
diseases.
In
this
study,
we
aimed
investigate
functional
roles
glucosamine
silibinin
cartilage
homeostasis
using
TC28a2
cell
line.
Western
blots
showed
that
suppressed
N-glycosylation
gp130,
EGFR,
N-cadherin
proteins.
Furthermore,
both
differentially
decreased
increased
proteins
such
Snail,
KLF4
cells.
We
observed
compounds
dose-dependently
induced
proliferation
Our
MitoSOX
DCFH-DA
dye
data
1
µM
mitochondrial
reactive
oxygen
species
(ROS)
generation
cytosol
ROS
generation,
whereas
JC-1
red
aggregates,
resulting
increase
red/green
fluorescence
intensity
ratio,
while
all
tested
concentrations
green
monomers,
decreases
ratio.
increasing
subG1
S
populations
decreasing
G1
G2/M
with
amounts
glucosamine,
led
increases
subG1,
S,
MTT
cytotoxicity
cells
dose-dependent
manner.
Regarding
endoplasmic
reticulum
stress,
expression
CHOP
level
p-eIF2α/eIF2α.
With
respect
O-GlcNAcylation
status,
reduced
levels
O-GlcNAc
transferase
hypoxia-inducible
factor
alpha.
examined
mRNAs
related
these
processes.
summary,
our
findings
demonstrated
modulated
cellular
proliferation,
membrane
potential,
cycle
profile,
autophagy.
Therefore,
conclude
not
only
mediate
modifications
but
also
regulate
processes
human
chondrocytes.
Journal of Community Medicine and Public Health Reports,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 29, 2024
Analysis
of
cells,
soluble
substances,
and
related
tissues
cells
that
cooperate
to
extend
life
continues
awaken
researchers'
curiosity.Because
its
incredible
complexity,
the
immune
system
is
opaque
outer
world.Scholars
strive
comprehend
several
facets
combine
give
a
comprehensive
picture
system's
operation.Thus,
various
tactics
have
been
acknowledged
for
their
role
in
development
immunotherapy,
including
genetic
approaches,
animal
models,
specialized
tools,
monoclonal
antibodies,
immunological
techniques.Much
research
has
done
understand
effects
immunotherapy
on
human
bodies
develop
range
treatments.Understanding
aspects
immunotherapy's
health,
sickness
management,
aging
made
possible
by
discoveries
about
cell
subsets,
membrane
molecules,
functions.A
thorough
grasp
systems
elements
provide
an
enabling
platform
welfare
management
resulted
from
focus
immunotherapy.Welfare
falls
under
purview
developing
immunotherapies,
which
served
as
cornerstone
sustainable
management.The
impact
application
explored
context
efficient
service
delivery,
ranging
cancer
tumor
effect
clarified
such
adopting
infections,
them
prophylactically,
using
antibodies
recombinant
proteins.Investing
has,
therefore,
responsible
change
how
we
treat
many
diseases.The
necessity
investigate
led
examining
adequately
managing
diseases.
Experimental Dermatology,
Год журнала:
2025,
Номер
34(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Glycosylation
is
a
common
and
complex
post‐translational
modification
(PTM)
of
proteins,
involving
the
attachment
glycans
under
regulation
various
enzymes
such
as
glycosyltransferases.
facilitates
correct
folding
peptide
chains,
modifies
protein
conformation
activity,
enhances
stability
influences
inter‐protein
interactions.
N‐glycosylation
O‐glycosylation
are
two
prevalent
forms,
encompassing
wide
range
modifications,
including
sialylation,
fucosylation
galactosylation.
In
skin
tumours,
abnormal
glycosylation
promotes
tumour
cell
proliferation,
migration,
invasion
metastasis,
anti‐tumour
immunity,
potentially
affects
immune
checkpoint
therapy.
inflammatory
autoimmune
diseases,
in
T
B
lymphocyte
subpopulations
regulates
antigen
recognition,
signal
transduction,
factor
secretion
immunoglobulin
function,
disrupting
system
homeostasis
impacting
biologic
therapy
efficacy.
correlates
with
severity
activity
serving
potential
biomarker
for
diagnosis,
condition
assessment
prognosis
determination.
This
review
provides
an
overview
role
melanoma,
basal
carcinoma,
squamous
psoriasis,
systemic
lupus
erythematosus,
dermatomyositis
aging.
It
analyses
biosynthetic
process
glycosylation,
elucidates
functional
changes
glycoproteins
their
metabolism,
offers
theoretical
basis
developing
new
targeted
therapies.
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
Abstract
Pelvic
organ
prolapse
(POP)
is
a
common
and
distressing
condition
affecting
women,
particularly
those
with
history
of
vaginal
delivery.
The
impact
extracellular
vesicles
derived
from
adipose‐derived
mesenchymal
stem
cells
(ADSC‐EVs)
on
pelvic
floor
tissue
injury
remains
unclear.
Due
to
their
short
half‐life
rapid
clearance
in
vivo,
ADSC‐EVs
lose
efficacy
quickly.
To
address
this,
an
injectable
tetra‐PEG
hydrogel
encapsulate
(PEG@EVs)
developed.
formed
by
tetra‐PEG‐NH
2
tetra‐PEG‐NHS
through
ammonolysis
reaction,
leading
the
formation
amide
bonds
within
seconds.
Vaginal
wall
POP
patients
shows
disruption
matrix,
lipid
peroxidation,
inflammation.
In
vitro,
significantly
reduce
H₂O₂‐induced
oxidative
stress,
oxidation,
apoptosis,
while
enhancing
expression
Nrf2
its
downstream
targets—CAT,
NQO1,
HO‐1,
SOD2.
also
upregulate
GPX4
SLC7A11,
reducing
mitochondrial
damage
mitigating
ferroptosis.
inhibitor
ML385
reverses
these
protective
effects.
rat
model
childbirth
injury,
PEG@EVs
treatment
promotes
nuclear
translocation,
induces
M1‐to‐M2
macrophage
conversion,
reduces
inflammation,
stimulates
collagen
deposition,
thereby
accelerating
repair.
findings
this
study
may
serve
as
foundation
for
early
targeted
intervention
POP,
representing
promising
therapeutic
approach.
Translational Cancer Research,
Год журнала:
2023,
Номер
12(12), С. 3538 - 3546
Опубликована: Дек. 1, 2023
Background:
Central
venous
catheters
(CVCs)
are
sometimes
superior
to
peripheral
vascular
access
for
chemotherapy.
line-associated
bloodstream
infections
(CLABSIs)
an
important
complication
of
CVCs
in
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
12
Опубликована: Авг. 26, 2024
Specific
protein
1
(Sp1)
is
pivotal
in
sustaining
baseline
transcription
as
well
modulating
cell
signaling
pathways
and
factors
activity.
Through
interactions
with
various
proteins,
especially
factors,
Sp1
controls
the
expression
of
target
genes,
influencing
numerous
biological
processes.
Numerous
studies
have
confirmed
Sp1’s
significant
regulatory
role
pathogenesis
cardiovascular
disorders.
Post-translational
modifications
(PTMs)
Sp1,
such
phosphorylation,
ubiquitination,
acetylation,
glycosylation,
SUMOylation,
S-sulfhydration,
can
enhance
or
modify
its
transcriptional
activity
DNA-binding
stability.
These
also
regulate
across
different
types.
crucial
regulating
non-coding
gene
proteins
response
to
pathophysiological
stimuli.
Understanding
PTMs
advances
our
knowledge
controlling
stability
during
disease
onset
progression.
It
aids
identifying
novel
pharmaceutical
targets
biomarkers
essential
for
preventing
managing
diseases.
Analytical and Bioanalytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 19, 2024
Abstract
Necrotizing
enterocolitis
(NEC)
is
a
life-threatening
condition
affecting
preterm
infants,
sometimes
necessitating
surgical
treatment.
This
study
aimed
to
analyze
differentially
expressed
proteins
(DEPs)
and
access
their
biological
clinical
significance
in
the
plasma
of
neonates
with
NEC.
Peripheral
blood
samples
were
collected
from
NEC
infants
at
various
time
points,
was
separated.
Data-independent
acquisition
(DIA)
technology
utilized
identify
DEPs
among
patients
different
stages.
Bioinformatic
analyses,
including
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes,
protein-to-protein
interaction
analyses
performed
on
DEPs.
External
datasets,
along
receiver
operating
characteristic
curves
gene
set
enrichment
analysis,
used
clinically
biologically
validate
findings.
between
pre-NEC
groups
indicated
reduced
protein,
heme,
nitrogen,
purine
nucleotide
biosynthesis
during
formation.
In
addition,
enriched
points
suggested
reconstructed
extracellular
matrix,
aberrant
B-lymphocyte
immune
responses,
decreased
glycosaminoglycan
levels
progression.
These
findings
both
validated
using
external
datasets.
Our
highlights
relevance
proteomics
patients.
demonstrates
key
pathways
involved
pathogenesis
establishes
DIA
mass
spectrometry
as
powerful
noninvasive
tool
for
evaluating
predicting
formation
Graphical
