Integrated Metabolome and Transcriptome Analyses Reveal the Mechanisms Regulating Flavonoid Biosynthesis in Blueberry Leaves under Salt Stress DOI Creative Commons
Bin Ma, Yan Song, Xinghua Feng

и другие.

Horticulturae, Год журнала: 2024, Номер 10(10), С. 1084 - 1084

Опубликована: Окт. 9, 2024

The flavonoids play important roles in plant salt tolerance. Blueberries (Vaccinium spp.) are extremely sensitive to soil increases. Therefore, improving the resistance of blueberries by increasing flavonoid content is crucial for development blueberry industry. To explore underlying molecular mechanism, we performed an integrated analysis metabolome and transcriptome leaves under stress. We identified 525 differentially accumulated metabolites (DAMs) stress vs. control treatment, primarily including members class. also 20,920 expressed genes (DEGs) based on data; these, 568 transcription factors (TFs) were annotated, bHLH123, OsHSP20, HSP20 TFs might be responsible leaf DEGs involved biosynthesis pathway significantly enriched at almost all stages Salt treatment upregulated expression most biosynthetic promoted accumulation flavonols, flavonol glycosides, flavans, proanthocyanidins, anthocyanins. Correlation suggested that 4-coumarate CoA ligases (4CL5 4CL1) flavonols (quercetin pinoquercetin) flavan-3-ol (epicatechin prodelphinidin C2) stress, respectively. 3′5′-hydroxylases (F3′5′H) regulate anthocyanin (cyanidin 3-O-beta-D-sambubioside delphinidin-3-O-glucoside chloride) biosynthesis, leucoanthocyanidin reductases (LAR) epicatechin C2 during Taken together, it one future breeding goals cultivate salt-resistant varieties genes, especially 4CL, F3′5′H, LAR promote leaves.

Язык: Английский

Epigenetic Evaluation of Melatonin Application in Bean (Phaseolus vulgaris L.) Genotypes Under Drought and Salt Stress Conditions DOI
Recep AYDINYURT,

Semra Yağcı,

Esra Yaprak

и другие.

Plant Molecular Biology Reporter, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

Язык: Английский

Процитировано

0
Genome-wide characterization of the PCO (Plant Cysteine ​​Oxidase) gene family in Phaseolus vulgaris L. and expression analysis under heavy metal stress DOI
Esma Yiğider

South African Journal of Botany, Год журнала: 2025, Номер 179, С. 246 - 260

Опубликована: Фев. 22, 2025

Язык: Английский

Процитировано

0
Investigating TIFY Genes for Salt Stress Adaptation in Quinoa (Chenopodium quinoa Willd.): A Genome-Wide Approach DOI Open Access
Esma Yiğider

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Год журнала: 2025, Номер 18(1), С. 213 - 228

Опубликована: Март 26, 2025

TIFY ailesi, TIFY, Jas ve GATA motiflerini içeren bitkilerin savunma mekanizmalarında stres faktörlerine karşı verdikleri yanıtta önemli rol oynayan bir gen ailesidir. ailesi birçok bitki türünde araştırılmış olmasına rağmen, kinoada henüz incelenmemiştir. Bu çalışmada, 16 Cq-TIFY geni tanımlanmış, bu genler Cq-TIFY-1’den Cq-TIFY-16’ya kadar numaralandırılarak yapısal işlevsel özellikleri karakterize edilmiştir. Tanımlanan proteinlerinin moleküler ağırlıkları 19,99 ile 48,59 kDa, amino asit sayıları 189 450, teorik izoelektrik noktaları ise 4,84 10,1 arasında değişmektedir. Filogenetik analiz sonuçlarına göre, genlerinin üç sınıfa ayrıldığı belirlenmiştir. Gen ailesinin farklı sınıflarındaki üyelerin yapılarının genellikle benzer olduğu Kinoa’da yedi segmental duplikasyon geçirmiş tanımlanmış olup, Ka/Ks analizi genlerin evrimsel süreçte arındırıcı (negatif) seçilime maruz kaldığını göstermiştir. Chenopodium quinoa, Arabidopsis thaliana Spinacia oleracea türleri arasındaki sinteni analizi, genleri açısından ilişki olduğunu ortaya koymuştur. Promotör sonucunda, genlerinde strese duyarlı hormonla ilişkili cis-elementlerin varlığı çıkarılmıştır. Araştırmada, RNA-seq verileri, tuz koşulları altında kök sürgün dokularında ifade modellerini incelemek için kullanılmıştır. Genlerin stresi altındaki profili köklerde sürgünlerde dokuya özgü olarak farklılık göstermiş ifadelerinde anlamlı artış sonuç, toleransı oynayabileceğini düşündürmüştür. çalışma, kinoadaki ailesine dair bilgimizi artırmakta klasik ıslah veya genetik mühendisliği yoluyla toleransını artırmaya yönelik temel oluşturmaktadır.

Процитировано

0
Genome-Wide Analysis of Vacuolar Iron Transporter (VIT) Gene Family in Phaseolus vulgaris L.: Functional Roles in Heavy Metal Stress DOI Open Access
Esma Yiğider

Journal of Agricultural Production, Год журнала: 2025, Номер 6(1), С. 15 - 31

Опубликована: Март 26, 2025

Vacuolar Iron Transporter (VIT) genes have been characterized and indicated to play critical roles in iron homeostasis various plants. Heavy metals pose a significant challenge bean cultivation, necessitating the development of heavy metal-resistant cultivars as key strategy mitigate their impacts. detoxification is crucial for plants survive adapt adverse environment caused by metal stress. The current study used bioinformatic tools characterize VIT gene bean, member legume family an important agricultural product, first time. identified 11 (PhvulVIT-1–PhvulVIT-11) bean's genome. These displayed molecular weights (MW) ranging from 16.48 28.92 kDa comprised 155–269 amino acid residues. distribution PhvulVIT on four chromosomes was not homogeneous, eight were observed be located chromosome 2. Gene duplication events suggested purifying selection primary evolutionary force, ensuring functional stability duplicated genes. Phylogenetic analysis classified into three clades, reflecting relationships with orthologs Arabidopsis thaliana Glycine max. Cis-regulatory element promoter regions revealed stress-responsive motifs like MYB, MYC, ABRE, which are essential plant responses environmental stresses phytohormone signaling. Additionally, expression patterns under conditions examined using RNAseq. This enhances our understanding nutrient stress adaptation, offering valuable insights crop improvement strategies, including biofortification stress-tolerant cultivars.

Язык: Английский

Процитировано

0
AcMYB176-Regulated AcCHS5 Enhances Salt Tolerance in Areca catechu by Modulating Flavonoid Biosynthesis and Reactive Oxygen Species Scavenging DOI Open Access
Yiqi Jiang, Nasiruddin Khan,

Akhtar Ali

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3216 - 3216

Опубликована: Март 30, 2025

High-salinity stress induces severe oxidative damage in plants, leading to growth inhibition through cellular redox imbalance. Chalcone synthase (CHS), a pivotal enzyme the flavonoid biosynthesis pathway, plays critical roles plant adaptation. However, molecular mechanisms underlying CHS-mediated salt tolerance remain uncharacterized Areca catechu L., tropical crop of high economic and ecological significance. Here, we systematically identified CHS gene family A. revealed tissue-specific salt-stress-responsive expression patterns, with AcCHS5 exhibiting most pronounced induction under salinity. Transgenic Arabidopsis overexpressing displayed enhanced compared wild-type characterized by elevated activities antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), increased accumulation, reduced reactive oxygen species (ROS) accumulation. Furthermore, transcription factor AcMYB176 as direct activator binding its promoter. Our findings demonstrate that AcMYB176-AcCHS5 regulatory module enhances orchestrating ROS scavenging. This study provides functional evidence adaptation highlights potential for improving resilience crops.

Язык: Английский

Процитировано

0
Integrated Metabolome and Transcriptome Analyses Reveal the Mechanisms Regulating Flavonoid Biosynthesis in Blueberry Leaves under Salt Stress DOI Creative Commons
Bin Ma, Yan Song, Xinghua Feng

и другие.

Horticulturae, Год журнала: 2024, Номер 10(10), С. 1084 - 1084

Опубликована: Окт. 9, 2024

The flavonoids play important roles in plant salt tolerance. Blueberries (Vaccinium spp.) are extremely sensitive to soil increases. Therefore, improving the resistance of blueberries by increasing flavonoid content is crucial for development blueberry industry. To explore underlying molecular mechanism, we performed an integrated analysis metabolome and transcriptome leaves under stress. We identified 525 differentially accumulated metabolites (DAMs) stress vs. control treatment, primarily including members class. also 20,920 expressed genes (DEGs) based on data; these, 568 transcription factors (TFs) were annotated, bHLH123, OsHSP20, HSP20 TFs might be responsible leaf DEGs involved biosynthesis pathway significantly enriched at almost all stages Salt treatment upregulated expression most biosynthetic promoted accumulation flavonols, flavonol glycosides, flavans, proanthocyanidins, anthocyanins. Correlation suggested that 4-coumarate CoA ligases (4CL5 4CL1) flavonols (quercetin pinoquercetin) flavan-3-ol (epicatechin prodelphinidin C2) stress, respectively. 3′5′-hydroxylases (F3′5′H) regulate anthocyanin (cyanidin 3-O-beta-D-sambubioside delphinidin-3-O-glucoside chloride) biosynthesis, leucoanthocyanidin reductases (LAR) epicatechin C2 during Taken together, it one future breeding goals cultivate salt-resistant varieties genes, especially 4CL, F3′5′H, LAR promote leaves.

Язык: Английский

Процитировано

2