Exogenous 24-epibrassinolide mitigates damage in grape seedlings under low-temperature stress

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Фев. 18, 2025

Grapes are cultivated worldwide and have a high economic value as fruit trees. However, winter frost damage spring cold limited the sustainability of table grape industry. A novel plant growth regulator, 24-epibrassinolide (EBR), exhibits an essential regulatory function in life cycles, especially its unique mechanism against various environmental stresses. We treated 'Lihongbao' grapes with exogenous EBR (0.2 μM), brassinazole (BRZ, 10 + BRZ μM +10 deionized water (CK). investigated effect on seedlings under low-temperature stress (4°C) at different periods (0 h, 12 24 48 96 h). explored physiological mitigation mechanisms injury by observing impacts treatment physical biochemical indices such phenotypes anatomical structures, photosynthetic characteristics, chlorophyll fluorescence parameters, antioxidant systems, osmoregulatory substances. Exogenous had inhibitory compared CK group. Based phenotype structure, leaves showed no signs loss or wilting. At h stress, lower epidermal thickness (LET), palisade tissue (PT), palisade-to-sea ratio (P/S), blade structural compactness (CTR) EBR-treated were significantly reduced 6.71%, 19.59%, 14.52%, 11.65% group, respectively. Chlorophyll (Chl a), b b), total total), carotenoids (carotenoid), transpiration rate (Tr), stomatal conductance (Gs) contents upregulated 30.24%, 48.52%, 39.75%, 34.67%, 704.66%, 277.27%, The intercellular CO2 concentration (Ci) non-photosynthetic burst coefficient (NPQ) downregulated 16.29% 25.83%, Glutathione (GSH) 33.63%, superoxide dismutase (SOD), peroxidase (POD), ascorbate (APX) activities increased 42.70%, 27.60%, 28.64%, hydrogen peroxide (H2O2), anion (O2·-), malondialdehyde (MDA) 29.88%, 23.66%, 47.96%, respectively, significantly. Catalase (CAT) activity 15.03%. Soluble sugar free proline 5.29% 19.44%, could effectively alleviate inhibition caused regulating system temperature. results offer theoretical basis for enhancing tolerance.

Язык: Английский

Divergent Photosynthetic Strategies of Lupinus polyphyllus and Helleborus viridis During Cold Acclimation and Freezing–Thaw Recovery

Plants, Год журнала: 2025, Номер 14(4), С. 607 - 607

Опубликована: Фев. 17, 2025

Low temperatures can significantly affect the growth of ornamental plants, emphasizing importance improving their cold tolerance. However, comparative studies on photosynthetic responses sun and shade plants to low remain limited. In this study, gas exchange, chlorophyll fluorescence in Photosystem II (PSII) I (PSI), antioxidant system, osmoregulator substance, lipid peroxidation were investigated plant Helleborus viridis (Hv) Lupinus polyphyllus (Lp) during acclimation (CA) freezing-thaw recovery (FTR). The CA treatment declined net rate (Pn) maximum photochemical efficiency PSII (Fv/Fm) Hv Lp, indicating photoinhibition occurred both species. exhibited a much better stability maintain Pn, Fv/Fm, carboxylation (CE) than Lp CA, suggesting that had greater resilience compared Lp. Furthermore, preferred actual (Y(II)), PSI (Y(I)) consistently provide necessary energy for carbon assimilation process, while tended divert dissipate excess by thermal dissipation cyclic electron flow CA. Moreover, there higher soluble sugar contents comparison These traits allowed recover cellular integrity after freezing stress. conclusion, reduced capacity led divergent strategies species, which finally resulted different tolerance recovery. findings insights into photoprotective response temperatures.

Язык: Английский

Progress of Grain Polyphenols: Biological Activities, Enrichment Methods, and Applications

Molecular Nutrition & Food Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

ABSTRACT Polyphenols are secondary metabolites derived from plant metabolism and possess diverse bioactivities health‐promoting functions. They extensively utilized in the food, pharmaceutical cosmetic industries, more. Recently, with increasing focus on healthy diets, grain polyphenols have been subject of extensive research. This review provides a comprehensive discussion biological functions polyphenols, which can be accomplished through modulation enzyme activities, signaling pathways, metabolic influence membrane structure. With regard to polyphenol extraction, highlights novel efficient extraction techniques. Additionally, it explores enrichment microbial action metabolism. Finally, offers summary current status future development potential food non‐food fields application. theoretical basis for research reference point their industrialization.

Язык: Английский