NEK2 supports porcine embryonic development by modulating the AKT signaling pathway DOI Creative Commons
Se‐Been Jeon,

Hyo‐Gu Kang,

Min Ju Kim

и другие.

Life Sciences, Год журнала: 2025, Номер unknown, С. 123640 - 123640

Опубликована: Апрель 1, 2025

Serine/threonine kinase NIMA-related 2 (NEK2) plays a crucial role in regulating the cell cycle and DNA damage response. This study explored mechanisms by which NEK2 inhibition affects porcine embryonic development. To explore of development, we used inhibitor JH295 AKT activator SC79. Various staining methods, including EdU, EU, OPP, TUNEL assay, real-time PCR, immunocytochemistry, Western blotting, were to identify effects on developmental competence, damage, related embryos. significantly reduced cleavage rate blastocyst formation rate. Abnormal development was associated with decreased expression genes zygotic genome activation levels OPP. Notably, p-AKT AKT, as well their transcript levels. While rates total number, all these reversed SC79 co-treatment. The proportions expanded survival trophectoderm numbers similarly restored control following combined treatment Furthermore, OPP completely had negative impact integrity, this effect inhibited Together, results suggest that signaling pathway.

Язык: Английский

NEK2 supports porcine embryonic development by modulating the AKT signaling pathway DOI Creative Commons
Se‐Been Jeon,

Hyo‐Gu Kang,

Min Ju Kim

и другие.

Life Sciences, Год журнала: 2025, Номер unknown, С. 123640 - 123640

Опубликована: Апрель 1, 2025

Serine/threonine kinase NIMA-related 2 (NEK2) plays a crucial role in regulating the cell cycle and DNA damage response. This study explored mechanisms by which NEK2 inhibition affects porcine embryonic development. To explore of development, we used inhibitor JH295 AKT activator SC79. Various staining methods, including EdU, EU, OPP, TUNEL assay, real-time PCR, immunocytochemistry, Western blotting, were to identify effects on developmental competence, damage, related embryos. significantly reduced cleavage rate blastocyst formation rate. Abnormal development was associated with decreased expression genes zygotic genome activation levels OPP. Notably, p-AKT AKT, as well their transcript levels. While rates total number, all these reversed SC79 co-treatment. The proportions expanded survival trophectoderm numbers similarly restored control following combined treatment Furthermore, OPP completely had negative impact integrity, this effect inhibited Together, results suggest that signaling pathway.

Язык: Английский

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