
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июнь 13, 2024
Abstract In the search for more effective prophylactic and possibly curative therapeutics against SARS-CoV-2, an historical-ethnobotanical approach was used to select plants described in Receptarium of Burkhard III von Hallwyl (RBH), influential recipe text from 16th century Switzerland. Ten species were identified based on specific historical uses presumably linked with treatment viral infections as well inflammatory conditions. For each plant candidate, aqueous hydroethanolic extracts have been produced. CellTiter-Glo® Luminescent Cell Viability Assay assess antiviral activity SARS-CoV-2 effect cell viability extracts. Of ten tested, four displayed ≥ 50% at 16.7 µg/ml acceptable (> 75%): Sambucus nigra L. (leaves), Viola odorata Geranium robertianum (arial parts) Artemisia vulgaris (aerial parts). The crude partitioned organic fractions further analyzed. ethyl acetate S. nigra, V. G. expressed significant nearly 100% 5.6 (P < 0.05). most potent inhibitory observed fraction (leaves) 87% 1.9 0.0001). Alongside bioactivity analysis phytochemical fingerprints made, aim understand important substance classes contained. Further investigations are required explore active principles. Our study shows that ethnopharmacological records traditional use potential herbal candidates coupled a rational screening process enables efficient plant-derived natural products SARS-CoV-2.
Язык: Английский