Sel skokhozyaistvennaya Biologiya, Год журнала: 2023, Номер 57(6), С. 1178 - 1187
Опубликована: Янв. 1, 2023
ВЛИЯНИЕ ВНЕКЛЕТОЧНЫХ ВЕЗИКУЛ ФОЛЛИКУЛЯРНОГО ПРОИСХОЖДЕНИЯ В СРЕДЕ СОЗРЕВАНИЯ НА СПОСОБНОСТЬ ООЦИТОВ КОРОВ К ЭМБРИОНАЛЬНОМУ РАЗВИТИЮ in vitroПОСЛЕ СТАРЕНИЯ И ЭКСТРАКОРПОРАЛЬНОГО ОПЛОДОТВОРЕНИЯ Е.Н.ШЕДОВА 1 , Г
Язык: Русский
Molecules, Год журнала: 2023, Номер 28(14), С. 5533 - 5533
Опубликована: Июль 20, 2023
The quality of oocytes determines the development potential an embryo and is dependent on their timely fertilization after ovulation. Postovulatory oocyte aging inevitable factor during some assisted reproduction technology procedures, which results in poor rates impairs development. We found that fisetin, a bioactive flavonol contained fruits vegetables, delayed postovulatory mice. Fisetin improved aged inhibited Sirt1 reduction oocytes. increased GSH level Sod2 transcription to inhibit ROS accumulation Meanwhile, fisetin attenuated aging-induced spindle abnormalities, mitochondrial dysfunction, apoptosis. At molecular level, decreased aberrant expression H3K9me3. In addition, levels Tfam genes Co2 Atp8 by upregulating Finally, inhibition reversed anti-aging effects fisetin. Taken together, Sirt1.
Язык: Английский
Процитировано
15
Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)
Опубликована: Окт. 15, 2024
Spermidine (SPD) is an intermediate compound in the polyamine metabolism which takes critical part a variety of cellular processes. In particular, it has been reported to exert anti-aging effects, suppress age-related diseases, and extend lifespan across species. However, whether favorable influence on quality postovulatory aged oocytes remains elusive.
Язык: Английский
Процитировано
6
Antioxidants, Год журнала: 2024, Номер 13(2), С. 248 - 248
Опубликована: Фев. 19, 2024
Postovulatory aging is known to impair the oocyte quality and embryo development due oxidative stress in many different animal models, which reduces success rate or pregnancy human assisted reproductive technology (ART) livestock timed artificial insemination (TAI), respectively. Salidroside (SAL), a phenylpropanoid glycoside, has been shown exert antioxidant antitumor effects. This study aimed investigate whether SAL supplementation could delay postovulatory process by alleviating stress. Here, we show that decreases malformation recovers mitochondrial dysfunction including distribution, membrane potential (ΔΨ) ATP content aged oocytes. In addition, treatment alleviates aging-caused such as higher reactive oxygen species (ROS) level, lower glutathione (GSH) reduced expression of antioxidant-related genes. Moreover, cytoplasmic calcium ([Ca2+]c) ([Ca2+]mt) SAL-treated oocytes return normal levels. Notably, suppresses aging-induced DNA damage, early apoptosis improves spindle assembly oocytes, ultimately elevating developmental rates quality. Finally, RNA-seq confirmatory experience showed promotes protective autophagy activating MAPK pathway. Taken together, our research suggests supplementing an effective feasible method for preventing preserving quality, potentially contributes improving successful ART TAI.
Язык: Английский
Процитировано
5
Antioxidants, Год журнала: 2022, Номер 12(1), С. 87 - 87
Опубликована: Дек. 30, 2022
Decrease in quality of postovulatory aged oocytes occurs due to oxidative stress and leads low fertilization development competence. It is one the main causes that exerting detrimental effect on success rate assisted reproductive technology (ART). Auraptene (AUR), a citrus coumarin, has been reported possess an antioxidant effects other tissues. In this study, we aimed confirm potential AUR delay oocyte aging process by alleviating stress. Superovulated mouse metaphase second meiosis (MII) were exposed 0, 1 or 10 μM for 12 h vitro aging. addition culture medium recovered abnormal spindle chromosome morphology mitigated mitochondrial distribution membrane (ΔΨ) oocytes. AUR-treated also showed suppressed stress, with lower reactive oxygen species (ROS) levels, higher glutathione (GSH) levels increased expression several genes involved antioxidation. Furthermore, significantly elevated embryo developmental rates. Oocytes exhibited morphokinetics very similar those control group. Altogether, these data allowed us conclude improved suggest as effective clinical supplement candidate prevent
Язык: Английский
Процитировано
15
Theriogenology, Год журнала: 2024, Номер 226, С. 387 - 399
Опубликована: Май 24, 2024
Язык: Английский
Процитировано
3
Communications Biology, Год журнала: 2024, Номер 7(1)
Опубликована: Авг. 27, 2024
High quality label-free imaging of oocytes and early embryos is essential for accurate assessment their developmental potential, a key element assisted reproduction procedures. To achieve this goal, we propose full-field optical coherence microscopy (FF-OCM), constructed as compact module fully integrated with commercial wide-field fluorescence microscope. Our system achieves sectioning in wide-field, high in-plane resolution 0.5 µm, sensitivity to backscattered light. demonstrate its capabilities, study live mouse at all important stages meiotic maturation embryogenesis. enables visualization intracellular structures, which are not visible common bright-field microscopy, i.e., internal structure nuclear apparatus, cytoskeletal filaments, cellular cortex, cytoplasmic protrusions, or zona pellucida features. Additionally, visualize quantify dynamics like stirring motion, envelope fluctuations nucleolus mobility. Altogether, that FF-OCM powerful tool research biology also holds great potential non-invasive time-lapse monitoring oocyte embryo reproduction. Full-field high-contrast high-resolution the
Язык: Английский
Процитировано
3
Journal of Animal Science, Год журнала: 2025, Номер 103
Опубликована: Янв. 1, 2025
Abstract Ovulation, fertilization, and embryo development are orchestrated synchronized processes essential for the optimal health of offspring. Postovulatory aging disrupts this synchronization impairs oocyte quality. In addition, causes fertilization loss poor development. This investigation aimed to unravel endpoint in vitro common carp (Cyprinus carpio) understand involvement apoptosis postovulatory death. It was observed that ability significantly declined (P < 0.001) at 8-h poststripping (HPS), subsequently triggering advanced stage aging, i.e., 48 HPS. process included an increase proapoptotic transcripts (fas, bax, cathepsin D, caspase 8, 9, 3a) 0.05), elevated levels 3 protein activation enzyme 0.001), a key player apoptosis, oocytes. Furthermore, effects on embryonic machinery were examined embryos 5-h postfertilization (HPF) 24 HPF derived from fresh aged Expression apoptotic genes activity remained basal level 5 (early blastula embryos) both contrast, zymogenic active forms increased 8-h-aged oocytes 0.01) compared with those Thus, intensified without affecting early embryos. Our findings demonstrate initiated by Fas/FasL system is important physiological accompanying carp.
Язык: Английский
Процитировано
0
Animals, Год журнала: 2025, Номер 15(3), С. 291 - 291
Опубликована: Янв. 21, 2025
Oocyte quality is crucial for successful fertilization and subsequent embryonic development. Post-ovulatory aging leads to reduced oocyte impaired embryogenesis, representing an unavoidable challenge in terms of certain assisted reproductive techniques. Diosmetin (DIOS), a natural flavonoid found lemons, spearmint, spider moss, exhibits antioxidant, anti-inflammatory, anti-apoptotic properties. However, its effects on the mature porcine oocytes vitro remain unexplored. This study investigated impact DIOS aging. In IVM medium, fresh were cultured 44 h, while 68 h. Following addition varying concentrations (0.01, 0.1, 1 μM) DIOS-treated group was The results demonstrated that 0.1 μM significantly improved blastocyst rates cell counts, reactive oxygen species (ROS), elevated glutathione (GSH) levels, enhanced mitochondrial function, decreased markers autophagy (LC3B), apoptosis (annexin V), endoplasmic reticulum stress (CHOP), senescence (SA-β-Gal). Furthermore, treatment upregulated expression relevant genes compared aged group. These findings suggest effectively delays
Язык: Английский
Процитировано
0
Current Biology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
American Journal of Obstetrics and Gynecology, Год журнала: 2025, Номер 232(4), С. S95.e1 - S95.e16
Опубликована: Апрель 1, 2025
The ovaries play a crucial role in both the endocrine health and fertility of adult women. fundamental functional units ovaries, primordial follicles, form during fetal development constitute ovarian reserve. Ovaries age prematurely comparison to other organs, with quality oocytes declining steeply prior entire reserve becoming depleted, usually around 50. Despite pivotal women's overall health, surprisingly little is known about mechanisms controlling follicle dormancy, growth activation, atresia, maturation, oocyte quality. Understanding function on cellular molecular level increasingly important for several reasons. First, global trend women delaying childbirth creates growing population patients wishing conceive when quantity their are already critically low. Second, conditions affecting such as polycystic ovary syndrome endometriosis, widespread, yet diagnosis treatment still present challenges. Lastly, advancements cancer therapies have increased number survivors who contend late complications hormonal balance. Clearly, better understanding diseases, aging, toxicity needed novel treatments, preventive therapies, safer pharmaceuticals. Human notoriously difficult obtain research due highly heterogeneous distribution follicles within tissue combined monthly cyclical changes further Single-cell profiling techniques creating new opportunities, enabling characterization small amounts unprecedented resolution. Here, we review literature single-cell adult, reproductive-age ovaries. majority 46 identified studies focused discarded assisted reproduction, only handful focusing tissue. overwhelming focus oocytes, although somatic cell niche undoubtedly plays an biology. Altogether, reveal unexpected diversity heterogeneity among germ cells, highlighting prevailing knowledge gaps basic As most common outcome it possible that part relates biology destined degenerate. absence spatial coordinates complicates interpretation roles significance various reported clusters. Accomplishing representative atlas will require merging these studies. However, direct comparisons challenging nonuniform nomenclature, differing sources, varying meta-data reporting, lack gold standards technical approaches. Although reports establish draft adult-fertile human more detailed metadata reporting be essential robust disease.
Язык: Английский
Процитировано
0