Identification of RSAD2 as a Key Biomarker Linking Iron Metabolism and Dendritic Cell Activation in Systemic Lupus Erythematosus Through Bioinformatics and Experimental Validation

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 3859 - 3878

Опубликована: Март 1, 2025

Systemic lupus erythematosus (SLE) is characterized by aberrant immune activation and disrupted iron metabolism, yet the molecular mediators that govern both processes remain unclear. This study aims to identify pivotal genes modulate responses delineate their contributions SLE pathogenesis. Differentially expressed related metabolism (IM-DEGs) were identified using datasets (GSE72326, GSE110169, GSE126307, GSE50772) from GEO database MSigDB. Functional enrichment analyses performed on (IM-Genes). A weighted gene co-expression network analysis was constructed hub genes, which further refined as potential biomarkers least absolute shrinkage selection operator method. The predictive value of these validated receiver operating characteristic (ROC) curves nomogram. CIBERSORT employed evaluate cell infiltration in SLE. Additionally, expression function RSAD2 confirmed RNA interference, quantitative real-time PCR, Western blotting techniques. Bioinformatics 4 biomarkers: RSAD2, MT2A, LCN2, LTF. exhibited highest clinical validity (AUC = 0.927) closely associated with classic diagnostic indicators. Its through ROC curve nomogram, highlighting its role Elevated observed peripheral blood mononuclear cells patients, positively correlating activated dendritic (DCs). Notably, Rsad2 knockdown markedly impaired DCs, evidenced suppressed inflammatory metabolism-related genes. Our findings suggest a biomarker therapeutic target for SLE, elucidating intricate relationship between dysregulation exacerbates

Язык: Английский

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Analysis of toxicity and mechanisms of busulfan in non-obstructive azoospermia: A genomic and toxicological approach integrating molecular docking, single-cell sequencing, and experimentation in vivo

Ecotoxicology and Environmental Safety, Год журнала: 2025, Номер 291, С. 117878 - 117878

Опубликована: Фев. 1, 2025

Environmental pollutants, including chemical contaminants, heavy metals, and pesticides, have been linked to adverse effects on male reproductive health, particularly sperm quality. Non-obstructive azoospermia (NOA) is a severe form of infertility caused by intrinsic testicular dysfunction, leading complete absence in the ejaculate. Busulfan, an alkylating chemotherapeutic agent widely used treat chronic myelogenous leukemia, known induce NOA through its toxic spermatogonial stem cells (SSCs). This study aimed identify key molecular targets pathways disrupted busulfan environment. By integrating docking, single-cell RNA sequencing, vivo experimentation, identified POLE LRAT as critical proteins. These proteins were shown interact strongly with busulfan, genomic instability increased germ cell apoptosis during spermatogenesis. Additionally, immune landscape NOA-affected testes revealed significant changes infiltration, potentially worsening condition. findings offer new insights into mechanisms busulfan-induced suggest potential therapeutic for preserving fertility chemotherapy patients. research advances understanding chemotherapy-induced toxicity emphasizes need strategies reduce negative fertility.

Язык: Английский

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Exploring the molecular mechanism of Tripterygium Wilfordii Hook F in treating systemic lupus erythematosus via network pharmacology and molecular docking

Clinical Rheumatology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Язык: Английский

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TF and TFRC regulate ferroptosis in swine testicular cells through the JNK signaling pathway

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142369 - 142369

Опубликована: Март 1, 2025

Язык: Английский

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