Sustainable keratoplasty models using agri-food waste: a hypothesis for transforming biowaste into biomaterials for tissue engineering research
Frontiers in Sustainable Food Systems,
Год журнала:
2025,
Номер
9
Опубликована: Март 26, 2025
With
a
growing
global
population,
ways
to
counterbalance
the
demand
for
meat
products
with
effective
food
security
and
waste
management
innovative
scalable
solutions.
Concurrently,
alarming
incidence
of
end-stage
organ
failure,
limited
availability
transplantable
organs,
directives
reduce
reliance
on
animal
testing
underscore
need
clinically
viable
sustainable
alternatives.
Our
approach
introduces
hypothesis-driven,
renewable
tissue
engineering
strategy
that
creates
low-cost
keratoplasty
models
derived
entirely
from
agri-food
waste.
Specifically,
we
hypothesize
abundant
by-products,
such
as
eyes
bladders,
provide
practically
unlimited
readily
available
supplies
corneal
tissues
urine-derived
stem
cells
(USCs)
can
be
repurposed
into
cost-effective,
relevant
Traditional
approaches
often
rely
cadaveric
tissues,
invasive
cell
sourcing,
or
expensive
commercial
lines,
which
require
complex
resource-intensive
processes,
including
high-end
bioreactor
systems
manufacturing
environments.
These
requirements
limit
widespread
adoption
technological
progress
needed
increase
supply
keratografts.
proposed
leverages
combination
post-mortem
bladder
harvesting,
in
turn
facilitates
decellularization,
non-invasive
USC
differentiation,
compartment-specific
recellularization
help
overcome
barriers
associated
traditional
seeding
generate
this
type
Overall,
our
perspective
suggests
way
devise
transformative
resource-efficient
engineering,
specifically
geared
toward
improving
outcomes
while
offering
broader
applications
regeneration
other
bodily
tissues/organs
biotechnological
innovation.
Язык: Английский
Advanced bioengineering strategies broaden the therapeutic landscape for corneal failure
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Ноя. 13, 2024
The
cornea
acts
as
the
eye
foremost
protective
layer
and
is
essential
for
its
focusing
power.
Corneal
blindness
may
arise
from
physical
trauma
or
conditions
like
dystrophies,
keratitis,
keratoconus,
ulceration.
While
conventional
treatments
involve
medical
therapies
donor
allografts—sometimes
supplemented
with
keratoprostheses—these
options
are
not
suitable
all
corneal
defects.
Consequently,
development
of
bioartificial
tissue
has
emerged
a
critical
research
area,
aiming
to
address
global
shortage
human
donors.
Bioengineered
corneas
hold
considerable
promise
substitutes,
potential
replace
either
specific
layers
entire
thickness
damaged
corneas.
This
review
first
delves
into
structural
anatomy
cornea,
identifying
key
attributes
necessary
successful
bioengineering.
It
then
examines
various
pathologies,
current
treatments,
their
limitations.
Finally,
outlines
primary
approaches
in
engineering,
exploring
cell-free,
cell-based,
scaffold-based
three
emerging
strategies
failure.
Язык: Английский