Functional and Structural Changes in Diaphragm Neuromuscular Junctions in Early Aging
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(16), С. 8959 - 8959
Опубликована: Авг. 17, 2024
Age-related
impairment
of
the
diaphragm
causes
respiratory
complications.
Neuromuscular
junction
(NMJ)
dysfunction
can
be
one
triggering
events
in
weaknesses
old
age.
Prominent
structural
and
functional
alterations
NMJs
were
described
elderly
rodents,
but
NMJ
changes
middle
age
remain
unclear.
Here,
we
compared
muscles
from
young
adult
(3
months)
middle-aged
(12
BALB/c
mice.
Microelectrode
recordings,
immunofluorescent
staining,
electron
microscopy,
myography,
whole-body
plethysmography
used.
We
revealed
presynaptic
(i)
postsynaptic
(ii)
changes.
The
former
included
an
increase
both
action
potential
propagation
velocity
neurotransmitter
release
evoked
by
low-,
moderate-,
high-frequency
activity
a
decrease
immunoexpression
synapsin
1
synaptic
vesicle
clustering.
latter
consisted
currents
via
nicotinic
acetylcholine
receptors
area
their
distribution.
These
correlated
with
increased
contractile
responses
to
moderate-
nerve
activation.
Additionally,
found
pattern
respiration
(an
peak
inspiratory
flow
tendency
elevation
tidal
volume),
which
imply
conclude
that
enhancement
neuromuscular
communication
(due
mechanism)
accompanied
improved
occurs
early
aging.
Язык: Английский
25-Hydroxycholesterol as a negative regulator of diaphragm muscle contractions via estrogen receptor and Ca2+ -dependent pathway
Histochemistry and Cell Biology,
Год журнала:
2025,
Номер
163(1)
Опубликована: Апрель 3, 2025
Язык: Английский
Short-Term Hypobaric Hypoxia Isoform-Specifically Protects Rat Skeletal Muscle Na,K-ATPase from Disuse-Induced Dysfunction
Journal of Evolutionary Biochemistry and Physiology,
Год журнала:
2024,
Номер
60(5), С. 1712 - 1724
Опубликована: Сен. 1, 2024
Язык: Английский
Impairment of Skeletal Muscle Contraction by Inhibitors of GABA Transporters
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12510 - 12510
Опубликована: Ноя. 21, 2024
γ-Aminobutyric
acid
(GABA)
has
a
significant
impact
on
the
functioning
of
not
only
central
but
also
peripheral
part
nervous
system.
Recently,
various
elements
GABAergic
signaling
system
have
been
discovered
in
area
neuromuscular
junction
mammals.
At
same
time,
functional
activity
membrane-bound
GABA
transporters
(GATs)
and
their
role
transmission
identified.
In
present
study,
performed
preparation
mouse
diaphragm,
effect
transporter
inhibitors
(nipecotic
β-alanine)
force
muscle
contraction
was
assessed.
It
found
that
presence
both
compounds
bathing
solution,
contractions
caused
by
stimulation
motor
nerve
dropped
30-50%.
However,
when
stimulated
directly,
no
contractile
observed.
The
depressant
β-alanine
induced
completely
abolished
Язык: Английский