Journal of Materials Chemistry B, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Pharmacotherapy is a treatment for rheumatoid arthritis with limitations, such as poor drug targeting, low effective dosage at the inflammatory site, and high systemic toxicity. The combined application of drug-loaded nanobubbles ultrasound technology provides new technique treatment.
Язык: Английский
Nature Communications, Год журнала: 2023, Номер 14(1)
Опубликована: Окт. 9, 2023
Abstract Skeletal disorders are commonly diagnosed by X-ray imaging, but the radiation limits its use. Optical imaging through near-infrared-II window (NIR-II, 1000–1700 nm) can penetrate deep tissues without risk, targeting of contrast agent is non-specific. Here, we report that lanthanide-doped nanocrystals passively target bone marrow, which be effective for over two months. We therefore develop high-resolution NIR-II method disease diagnosis, including 3D instrumentation to show intravital morphology. demonstrate monitoring 1 mm defects with spatial resolution comparable result. Moreover, reveal early onset inflammation as synovitis in stage rheumatoid arthritis, micro computed tomography (μCT) diagnosis osteoarthritis, symptoms osteophyte and hyperostosis knee joint.
Язык: Английский
Процитировано
27
Molecules, Год журнала: 2023, Номер 28(16), С. 6125 - 6125
Опубликована: Авг. 18, 2023
Rheumatoid arthritis (RA) is an autoimmune disease characterized by bone and joint degeneration. Existing anti-inflammatory chemotherapy drugs offer temporary relief but come with undesirable side effects. Herbal medications have shown positive effects on RA symptoms minimal adverse reactions. In this study, we investigated the potential of Nyctanthes arbor-tristis (NAT) through in vitro silico research. Hydroethanolic extracts harsingar were prepared using reflux method, containing alkaloids, phenol, saponin, steroids, proteins, tannins, terpenoids, carbohydrates, glycosides, flavonoids, which exhibited TPC (98.56 ± 0.46 mg GAE/g) TFC (34.51 0.45 CE/g). LC–MS/MS analyzes active compounds extract. NAT best scavenging capabilities at 1 mg/mL anti-oxidant anti-arthritic activity. Maximum splenocyte proliferation occurred 250 µg/mL. cell studies revealed downregulation TNF-α upregulation IL-10. Additionally, study demonstrated that bioactive constituents targets bind favorable binding affinity. These findings demonstrate exerting effects, as supported studies. Further mechanistic research necessary to validate therapeutic all phytoconstituents treatment.
Язык: Английский
Процитировано
16
Frontiers in Pharmacology, Год журнала: 2024, Номер 15
Опубликована: Фев. 13, 2024
Background: Rheumatoid arthritis (RA) is an erosive-destructive inflammation of the joints, and chronic, long-term stiffness deformation induced by RA are some symptoms that difficult to treat. Dexamethasone (DEX) melittin (MLT) two interesting anti-inflammatory substances, both which possess effects exerted through suppression immune system. The purpose this study was explore role MLT in treatment DEX as well clarify influence on efficacy side DEX. Method: rats were injected with Complete Freund’s Adjuvant (CFA) induce arthritis, followed different doses and/or MLT. relevant indexes paw determined, appetite, growth status, cytokine levels, organ coefficient evaluated. In addition, paraffin sections joint tissues prepared analyze pathological changes. Result: exhibited effects, notably hindering feed intake growth, inducing lesions rats. significantly reduced promoted its efficacy. combination demonstrated a synergistic treatment, showing advantages detumescence reduction, pro-inflammatory inhibition, internal improvement. Conclusion: Thus, adjuvant rats, offering approach reduce use dosage
Язык: Английский
Процитировано
6
Inflammation Research, Год журнала: 2022, Номер 71(12), С. 1477 - 1488
Опубликована: Окт. 26, 2022
Язык: Английский
Процитировано
27
Molecules, Год журнала: 2023, Номер 28(6), С. 2483 - 2483
Опубликована: Март 8, 2023
Rheumatoid arthritis (RA) is a chronic and autoimmune disease characterized by inflammation, dysfunction, cartilage bone destruction. In this review, we summarized the available reports on protective effects of Ganoderma lucidum polysaccharides (GLP) RA in terms anti-inflammatory, immunomodulatory, anti-angiogenic osteoprotective effects. Firstly, GLP inhibits synovial fibroblast (RASF) proliferation migration, modulates pro- anti-inflammatory cytokines reduces inflammation. Secondly, regulates differentiation antigen-presenting cells such as dendritic cells, phagocytosis mononuclear macrophages nature killer (NK) ratio M1, M2 related inflammatory cytokines. addition, produced activities balancing humoral cellular immunity, regulating immunoglobulin production, modulating T B lymphocyte proliferative responses cytokine release, exhibiting immunomodulatory Thirdly, angiogenesis through direct inhibition vascular endothelial cell induction death indirect growth factor (VEGF) production cells. Finally, can inhibit matrix metalloproteinases promote osteoblast formation, exerting articular cartilage. It suggested that may be promising agent for treatment RA.
Язык: Английский
Процитировано
15
Carbohydrate Polymers, Год журнала: 2023, Номер 327, С. 121683 - 121683
Опубликована: Дек. 11, 2023
Язык: Английский
Процитировано
15
Journal of Chromatography B, Год журнала: 2023, Номер 1230, С. 123917 - 123917
Опубликована: Окт. 29, 2023
Janus kinase inhibitors (JAKi) are oral small molecules used in the treatment of a broad spectrum autoimmune and myeloproliferative diseases. JAKi exhibit significant intra- inter-individual pharmacokinetic variabilities, due to fluctuations compliance with treatments their metabolism essentially driven by cytochrome P450 enzymes. Intrinsically, have dose-response relationship narrow therapeutic index: drug monitoring (TDM) is expected optimize adapt dosage regimen order resolve problems efficacy tolerance linked dose safety. A sensitive analytical method using multiplex high-performance liquid-chromatography coupled tandem mass spectrometry (HPLC-MS/MS) was developed validated for simultaneous quantification plasma 6 major currently JAKi, namely abrocitinib, baricitinib, fedratinib, ruxolitinib, tofacitinib, upadacitinib. Plasma samples subjected protein precipitation MeOH, stable isotopically labelled internal standards. The separation supernatants diluted 1:1 ultrapure H2O performed C18 column Xselect HSS T3 2.5 µm, 2.1x150 mm mobile phase composed formic acid (FA) 0.2% acetonitrile (+FA 0.1%) gradient mode. run time assay 7 min. drugs were monitored electrospray ionization positive mode followed triple-stage quadrupole MS/MS analysis. according SFSTP ICH guidelines over clinically relevant concentration ranges (0.5-200 ng/mL baricitinib upadacitinib; 1-400 tofacitinib; 0.5-400 10-800 fedratinib). This HPLC-MS/MS achieved good performances term trueness (91.1-113.5%), repeatability (3.0-9.9%), intermediate precision (4.5-11.3%). We highly upadacitinib human plasma. will be applied prospective clinical studies determine whether TDM programs based on residual concentrations can recommended disease-specific ranges.
Язык: Английский
Процитировано
13
Frontiers in Molecular Biosciences, Год журнала: 2024, Номер 11
Опубликована: Март 7, 2024
The heterocycle compounds, with their diverse functionalities, are particularly effective in inhibiting Janus kinases (JAKs). Therefore, it is crucial to identify the correlation between complex structures and biological activities for development of new drugs treatment rheumatoid arthritis (RA) cancer. In this study, a set 28 heterocyclic compounds selective JAK1 JAK3 was employed construct quantitative structure-activity relationship (QSAR) models using multiple linear regression (MLR). Artificial neural network (ANN) were QSAR models. robustness stability assessed through internal external methodologies, including domain applicability (DoA). molecular descriptors incorporated into model exhibited satisfactory receptor-ligand JAKs observed X-ray crystallography, making interpretable predictive. Furthermore, pharmacophore ADRRR ADHRR designed each JAK3, proving discriminating active decoys. Both demonstrated good performance identifying an ROC 0.83 0.75 model. Using model, most promising selected based on strong affinity compared studied series JAK3. Notably, pharmacokinetic, physicochemical properties, (As ZINC79189223 ZINC66252348) found be consistent therapeutic effects RA, owing non-toxic, cholinergic nature, absence P-glycoprotein, high gastrointestinal absorption, ability penetrate blood-brain barrier. ADMET properties assessed, dynamics MM/GBSA analysis revealed these molecules.
Язык: Английский
Процитировано
5
ACS Applied Materials & Interfaces, Год журнала: 2024, Номер unknown
Опубликована: Апрель 10, 2024
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by abnormal activation of CD4
Язык: Английский
Процитировано
5
Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 178, С. 117219 - 117219
Опубликована: Июль 30, 2024
A transdermal delivery system offers high bioavailability and favorable patient adherence, constituting an optimal approach for localized administration in rheumatoid arthritis (RA) treatment. However, the stratum corneum (SC) impedes efficiency of conventional drug systems. Microneedles (MNs) can temporarily create micropores within SC, enabling distribution via bypassing this barrier enhancing effectiveness. Notably, MNs provide a painless method through skin may directly modulate inflammation immune cells by delivering drugs lymphatic during administration. MN is not suitable with low water solubility stability. Additionally, major concerns exist regarding safety using highly cytotoxic drugs, given that it could result local concentration at site. While exhibit some degree targeted to inflammatory environment, their targeting remains suboptimal. Nanoformulations have potential significantly address limitations RA treatment improving targeting, solubility, stability, biocompatibility. Therefore, review provides concise overview advantages, disadvantages, mechanisms different types It specifically focuses on application advantages combining nanoformulation summarizes current trends development nanoformulations combined field treatment, offering theoretical support future advancements clinical applications.
Язык: Английский
Процитировано
5