Experimental Eye Research, Год журнала: 2024, Номер 251, С. 110204 - 110204
Опубликована: Дек. 9, 2024
Язык: Английский
Biochemical Pharmacology, Год журнала: 2023, Номер 218, С. 115909 - 115909
Опубликована: Ноя. 4, 2023
Язык: Английский
Процитировано
21
International Immunopharmacology, Год журнала: 2024, Номер 134, С. 112231 - 112231
Опубликована: Май 12, 2024
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 307 - 307
Опубликована: Янв. 1, 2025
Cataracts remain the leading cause of visual impairment worldwide, yet underlying molecular mechanisms, particularly in age-related cataracts (ARCs), are not fully understood. The Notch signaling pathway, known for its critical role various degenerative diseases, may also contribute to ARC pathogenesis, although specific involvement is unclear. This study investigates regulating ferroptosis lens epithelial cells (LECs) and impact on progression. RNA sequencing anterior capsule samples from patients revealed a significant downregulation signaling, coupled with an upregulation ferroptosis-related genes. Notch1 expression decreased, while markers increased age-dependent manner. In vitro, alleviated by decreasing ferritin heavy chain 1 (FTH1) p53 levels enhancing nuclear factor erythroid 2-related 2 (Nrf2), glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11). Conversely, inhibition exacerbated ferroptosis, as evidenced reduced Nrf2, GPX4, SLC7A11 expression. These findings suggest that promotes LECs impairing Nrf2/GPX4 antioxidant thereby contributing development. offers new insights into pathogenesis highlights pathway potential therapeutic target preventing or mitigating
Язык: Английский
Процитировано
0
Experimental Eye Research, Год журнала: 2025, Номер 254, С. 110306 - 110306
Опубликована: Фев. 20, 2025
Язык: Английский
Процитировано
0
Journal of Medicinal Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 10, 2025
Ferroptosis is a nonapoptotic form of cell death discovered in 2012. Noninvasive treatments regulating ferroptosis are important for wide range diseases. Among the noninvasive treatments, sonodynamic therapy (SDT) has become promising due to its strong tissue penetration and few side effects. In recent years, targeted drug delivery platforms constructed on basis SDT have provided an efficient mode regulation ferroptosis. Based latest research reports, this Perspective introduces basic mechanism influencing factors therapeutic effects, elucidates significance ferroptosis-targeted SDT, summarizes studies through different pathways. We also present innovative composite ultrasound-responsive platforms. Finally, brief summary outlook based current presented.
Язык: Английский
Процитировано
0
Frontiers in Bioscience-Landmark, Год журнала: 2025, Номер 30(3)
Опубликована: Март 18, 2025
Background: Diabetes retinopathy (DR) represents a microvascular disease in diabetes. Growth arrest-specific 1 (GAS1) is differentially expressed rat retinal Müller cells under high glucose (HG) conditions, and its promotion of ferroptosis contributes to cell death. However, the influence GAS1 DR elusive. Herein, we aimed investigate effect potential mechanism based on GAS1-mediated DR. Methods: After HG treatment, genes were analyzed by transcriptome sequencing followed Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) analyses; finally, was selected. The effects knockdown/overexpression nuclear factor erythroid 2-related (Nrf2) silencing viability, apoptosis, lipid peroxidation, Fe2+, oxidative stress levels HG-induced/transfected measured Cell Counting Kit-8 (CCK-8) assay, flow cytometry, commercial reagent kits. Nrf2, especially GAS1, Kelch-like ECH-associated protein (Keap1) expressions cells, determined quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot. Results: treatment decreased viability glutathione (GSH) increased reactive oxygen species (ROS), disulfide (GSSG), malondialdehyde (MDA), stress, Fe2+ (p < 0.01). also upregulated Keap1, total Nrf2 while downregulating 0.001). downregulation enhanced GSH levels, expression reducing ROS, GSSG, MDA, HG-treated 0.001), whereas overexpression had opposite effects. Additionally, reversed impact 0.05). Conclusion: inhibits Keap1/Nrf2 signaling transduction activating cells; thus, this study can aid setting stage for novel methods against
Язык: Английский
Процитировано
0
International Ophthalmology, Год журнала: 2025, Номер 45(1)
Опубликована: Апрель 2, 2025
Язык: Английский
Процитировано
0
Cell Biology and Toxicology, Год журнала: 2025, Номер 41(1)
Опубликована: Апрель 2, 2025
Sodium fluoride-induced ocular damage constitutes a significant public health concern globally; however, the precise molecular mechanisms underlying this issue remain obscure. This study aims to investigate effects of sodium fluoride on myopia and offer novel theoretical foundations for future strategies in prevention control. The experimental data showed that could promote progression, through bioinformatics analysis, we found affect ferroptosis pathway. Western blotting redox kit assays further confirmed activates We also demonstrated PIEZO1 plays crucial role myopia, inhibitor (GsMTx4) can inhibit Subsequently, identified as potential target baicalin, which inhibited expression vivo vitro, by docking modeling CETSA assays. Finally, baicalin via PIEZO1. Taken together, our findings indicate progression activating pathway PIEZO1, targeting delay may provide new drug treatment future.
Язык: Английский
Процитировано
0
Survey of Ophthalmology, Год журнала: 2023, Номер 69(3), С. 303 - 310
Опубликована: Ноя. 23, 2023
Язык: Английский
Процитировано
10
Free Radical Biology and Medicine, Год журнала: 2024, Номер unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
3